Article
Pharmacology & Pharmacy
Olga Lavinda, Prashiela Manga, Seth J. Orlow, Timothy Cardozo
Summary: The study demonstrates the importance of the TYR-TYRP1-TYRP2 complex in melanogenesis, suggesting that the formation of this complex may help stabilize the TYR protein and influence melanin production.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Vilas B. Dongre, Sudhakar P. Awandkar, Vishwas M. Salunke, Mahesh B. Kulkarni, Laxmikant S. Kokate, Shyam B. Kale, Ravji R. Mugale
Summary: This study investigated the allelic and genotypic frequencies in the coding region of the TYR gene, as well as gene expression profile, in Deoni animals of western India. The results showed that the genotypic frequency of the TYR gene was not significant across different strains, genders, age groups, and levels of partial albinism. The gene expression of tyrosinase was significantly higher in young animals compared to adult animals, and it was higher in animals with low intensity of partial albinism.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Monika B. Dolinska, David E. Anderson, Yuri V. Sergeev
Summary: Tyrosinase related protein 1 (TYRP1) is crucial for melanin synthesis in melanocytes, and mutations to the TYRP1 gene can cause a rare disease called oculocutaneous albinism type 3 (OCA3). We investigated the impact of genetic mutations on TYRP1 structure and function by engineering mutant variants mimicking OCA3-related changes. Our results showed that some mutants maintained native conformation and enzymatic activity, while others misfolded or disrupted essential interactions, explaining the phenotype changes associated with OCA3 mutations.
Article
Microbiology
Daniella van der Walt, Emma T. Steenkamp, Brenda D. Wingfield, P. Markus Wilken
Summary: Hybridization is an important driver of evolution and adaptation, and closely related species of Ceratocystis have been shown to hybridize. However, previous studies using self-sterile strains and a laboratory-generated sterile isolate type may have impacted conclusions. This study successfully produced hybrids between self-fertile isolates of Ceratocystis species and provided evidence of biparental mitochondrial inheritance. Further research is needed to investigate the role of hybridization in the speciation of Ceratocystis species and the effect of mitochondrial conflict.
Article
Biology
Takumi Wagatsuma, Eisuke Suzuki, Miku Shiotsu, Akiko Sogo, Yukina Nishito, Hideya Ando, Hisashi Hashimoto, Michael J. Petris, Masato Kinoshita, Taiho Kambe
Summary: Zinc transporters ZNT5-6 and ZNT7 are necessary for pigmentation and the expression and function of tyrosinase-related protein TYRP1 in human melanoma cells and medaka fish. Loss of ZNT5-6 and ZNT7 function leads to hypopigmentation, immature melanosomes, and reduced melanin content, similar to TYRP1 dysfunction. The requirement of ZNT5-6 and ZNT7 for TYRP1 expression is conserved in human, mouse, and chicken orthologs.
COMMUNICATIONS BIOLOGY
(2023)
Article
Veterinary Sciences
Zhichao Yu, Zhiguo Zhao, Linjun Chen, Han Yan, Qiang Cui, Xianghong Ju, Yanhong Yong, Xiaoxi Liu, Xingbin Ma, Guanhua Zhang
Summary: The study developed a novel ddPCR assay for detecting BoHV-1 DNA in semen samples. The assay showed high sensitivity, specificity, and accuracy for mixed samples, providing a new diagnostic tool for BoHV-1 detection.
BMC VETERINARY RESEARCH
(2022)
Article
Microbiology
Asmaa Magouz, Ismail El-Kon, Enrique Raya-alvarez, Enas Khaled, Noura Alkhalefa, Alaa S. Alhegaili, Manal F. El-khadragy, Ahmad Agil, Ehab Kotb Elmahallawy
Summary: This study reports the prevalence and molecular analysis of CPV-2 variants collected from El-Gharbia and Kafrelsheikh governorates in the Delta of Egypt. The results showed that CPV-2a and CPV-2c are circulating in the study area.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Yoya Vashi, Ankit Magotra, Dipjyoti Kalita, Santanu Banik, Nihar R. Sahoo, Sanjay K. Gupta, Soumen Naskar
Summary: Improvement in litter traits is crucial for increasing economic income of pig farmers in developing countries. This study investigated the relationship between various genes and litter traits in indigenous pigs in India, revealing that the FSH beta gene may be a potential genetic marker for litter traits in these breeds.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Multidisciplinary Sciences
Autumn R. Brown, Kaylee Comai, Dominic Mannino, Haily McCullough, Yamini Donekal, Hunter C. Meyers, Chiron W. Graves, Hannah S. Seidel
Summary: This study investigates three color morphs in ball pythons and identifies genetic variants associated with each morph, offering insights into the genetics of coloration in reptiles.
Article
Microbiology
Francesca Oberto, Emanuele Carella, Claudio Caruso, Pier Luigi Acutis, Davide Lelli, Luigi Bertolotti, Loretta Masoero, Simone Peletto
Summary: The aim of this study was to develop a qualitative PCR assay that allows the discrimination of BuHV-1, BoHV-1 and BoHV-5. Specific nucleotide sequences of BuHV-1, BoHV-1 and BoHV-5 were identified through the alignment of homologous genes. The design of the primers and the optimization of the PCR assay were focused on the target sequences located on the portions of gD, gE and gG genes. This qualitative PCR assay allowed the differentiation of BoHV-1 and BoHV-5 infections in both cattle and water buffaloes, as well as heterologous BuHV-1 infections in bovine.
Article
Plant Sciences
Meng Ni, Xiaofang Yi, Qin Wang, Juan Wang, Shuang Wang, Liwang Liu, Liang Xu, Yan Wang
Summary: The study identified S haplotypes among 79 cultivated radish genotypes by analyzing the gene sequence and using PCR-RFLP method. The results showed that S haplotypes could be divided into Class I, Class II, and Class I/II, and three new haplotypes were identified in radish. By digestion of DNA fragments, homozygous S haplotypes could be easily distinguished. The molecular identification was consistent with field pollination and pollen tube germination results.
Article
Genetics & Heredity
Jakaria Jakaria, Wenny Ladhunka Nur Aliyya, Riyadi Ismail, Sri Yuni Siswanti, Mokhamad Fakhrul Ulum, Rudy Priyanto
Summary: This study investigated single-nucleotide polymorphisms (SNPs) and 11-bp deletions in the coding region of the MSTN gene in Belgian Blue, Peranakan Ongole, and their crossbred cattle. Although four SNPs and an 11-bp deletion were found, they were not able to differentiate between normal and double-muscled phenotypes accurately.
Review
Infectious Diseases
Lili Hao, Chaoxi Chen, Keith Bailey, Leyi Wang
Summary: Bovine kobuvirus (BKV), a non-enveloped RNA virus, has been detected in 13 countries across four continents, potentially causing neonatal calf diarrhoea. Further research, including animal challenge studies, is urgently needed to understand the pathogenicity of BKV.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Agriculture, Dairy & Animal Science
Junyan Bai, Xinle Wang, Jingyun Li, Mengke Chen, Fanlin Zeng, Xiaoning Lu, Yuhan He
Summary: This study analyzed the VIPR-1 gene polymorphism and its association with growth traits in quail. Two SNPs were detected in the VIPR-1 gene. The association analysis showed that SNPs were significantly associated with growth traits in both SV and FG strains. Haplotype combinations based on these SNPs also had significant associations with various growth traits. In conclusion, the VIPR-1 gene can serve as a molecular genetic marker for improving growth traits in quail.
Article
Biochemistry & Molecular Biology
Miral M. Refeat, Taghreed Shalabi, Hala T. El-Bassyouni, Mai Shaker
Summary: This study investigated the association of SNPs of ESR1 and PR genes with RPL in an Egyptian population, showing higher significance of ESR1 gene SNPs (rs2234693) and (rs9340799) in RPL cases compared to controls, as well as significantly increased frequencies of PR gene variants S344T (rs3740753) and H770H (rs1042839) in women. A new P352Q polymorphism was also observed. There is a significant association of ESR1 and PR gene SNPs with RPL, warranting further research to verify these findings.
MOLECULAR BIOLOGY REPORTS
(2021)
