Journal
ANIMAL GENETICS
Volume 39, Issue 3, Pages 321-324Publisher
WILEY
DOI: 10.1111/j.1365-2052.2008.01728.x
Keywords
expressed sequence tag; Haliotis midae; single nucleotide polymorphism
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In this study, we describe the first set of SNP markers for the South African abalone, Haliotis midae. A cDNA library was constructed from which ESTs were selected for the screening of SNPs. The observed frequency of SNPs in this species was estimated at one every 185 bp. When characterized in wild-caught abalone, the minor allele frequencies and F-ST estimates for every SNP indicated that these markers may potentially be useful for population analysis, parentage assignment and linkage mapping in Haliotis midae. No linkage disequilibrium was observed between SNPs originating from different EST sequences. These SNPs, together with additional SNPs currently being developed, will provide a useful complementary set of markers to the currently available genetic markers in abalone.
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