Article
Veterinary Sciences
Lukas Garrido Albertino, Ana Luisa Holanda Albuquerque, Julia Franco Ferreira, Joao Pedro Marmol Oliveira, Alexandre Secorun Borges, Thais Helena Constantino Patelli, Jose Paes Oliveira-Filho
Summary: This study aimed to verify the allelic frequency of APAF1 mutation in Brazilian Holstein cattle. The results showed that the mutation responsible for APAF1 was not detected in the population assessed in this study. The ARMS-PCR technique can be used as a relatively simple and economical method to determine the animals' genotype.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agronomy
Xubin Lu, Hui Jiang, Abdelaziz Adam Idriss Arbab, Bo Wang, Dingding Liu, Ismail Mohamed Abdalla, Tianle Xu, Yujia Sun, Zongping Liu, Zhangping Yang
Summary: This study analyzed the genetic characteristics of somatic cell score (SCS) in Chinese Holstein cattle and identified candidate genes and biological processes related to cow mastitis. The heritability of SCS varied between 0.07 and 0.24, decreasing with parity. Six significant SNPs were found, including one in the CD44 gene that may alter protein activity. Additionally, 32 genes near significant SNPs were identified and involved in various pathways and processes related to mastitis development. Nine candidate genes (PKD2, KCNAB1, SLC35A4, SPP1, IBSP, CD14, CD44, MAPK10, and ABCG2) were highlighted for potential roles in mastitis. These findings can contribute to molecular breeding efforts and reducing mastitis incidence in Chinese Holstein cattle.
Article
Agriculture, Dairy & Animal Science
Xiaohua Du, Huitong Zhou, Xia Liu, Yunhai Li, Jonathan G. H. Hickford
Summary: Lipins, particularly lipin-1, play a role in regulating lipid metabolism in tissues, with lipin-1 being highly expressed in the mammary gland of dairy cows. Variations in the LPIN1 gene were found to be associated with milk fat and protein synthesis, suggesting a potential as a gene-marker for improving milk production traits.
Article
Agriculture, Dairy & Animal Science
Mudasir Nazar, Ismail Mohamed Abdalla, Zhi Chen, Numan Ullah, Yan Liang, Shuangfeng Chu, Tianle Xu, Yongjiang Mao, Zhangping Yang, Xubin Lu
Summary: Udder conformation traits are economically important for dairy animals, and understanding their genetic architecture is crucial for genetic improvement and long-term selection in Holstein Friesian cattle. By conducting a GWAS on udder traits in Chinese Holstein cattle, several significant SNPs and candidate genes associated with udder structure traits were identified. These findings provide valuable information for improving the genetic information, health, longevity, and production of dairy cattle.
Article
Veterinary Sciences
Md. Yousuf Ali Khan, Abdullah I. Omar, Yuwei He, Shaohu Chen, Shengli Zhang, Wei Xiao, Yi Zhang
Summary: A study in Chinese Holstein herds revealed the presence of multiple genetic defects, with 27.43% of cows carrying at least one defect. Routine molecular testing and effective management are necessary to reduce the spread of genetic defects.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Yuechuan Huang, Hailiang Zhang, Cheng Mei, Minglu Yang, Shanjiang Zhao, Huabin Zhu, Yachun Wang
Summary: This study analyzed the phenotypic and genetic characteristics of in vitro embryo production traits in Chinese Holstein cattle, and investigated the factors influencing the production efficiency of in vitro embryo production. The results showed that the traits were significantly influenced by season and donor age, and exhibited heritability with low to high estimates. The count traits and proportion traits reflected different aspects of embryo production and should be considered in genetic selection to improve efficiency.
Article
Multidisciplinary Sciences
Jun Chai, Qinglu Wang, Bo Qin, Shengkui Wang, Youtao Wang, Muhammad Shahid, Kai Liu, Yifang Zhang, Weijie Qu
Summary: The study found a significant association between NOS2A gene polymorphisms and the risk of bTB infection in Chinese Holstein cattle. Certain genotypes were associated with susceptibility, while others were linked to resistance. Additionally, different single-nucleotide polymorphisms in NOS2A have a significant impact on the susceptibility/resistance pattern to tuberculosis.
Article
Agriculture, Multidisciplinary
Ishaku L. Haruna, Yunhai Li, Huitong Zhou, Jon G. H. Hickford
Summary: This study found that variations in the leptin gene can impact milk traits in cows, particularly affecting protein content. While there was no association between LEP genotypes and milk yield and fat percentage, cows with the AB genotype had lower protein content in their milk. This research suggests that variations in exon 3 of LEP may be useful for selecting cows for improvement in key milk traits.
NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Maj Beldring Henningsen, Matt Denwood, Carsten Thure Kirkeby, Soren Saxmose Nielsen
Summary: This scientific study aimed to compare somatic cell count (SCC) curves throughout lactation in dairy cows according to PCR test results for four major intramammary infection (IMI) pathogens. The findings revealed that cows testing positive for IMI pathogens had higher mean SCC across all parity groups and lactations. This study is important for the selective dry cow therapy treatment of intramammary infection.
Article
Agriculture, Dairy & Animal Science
Shuanghang Liu, Tingxian Deng, Liping Hua, Xinzhe Zhao, Hanxiao Wu, Peihao Sun, Mingxiao Liu, Shujun Zhang, Liguo Yang, Aixin Liang
Summary: This study identified significant genetic effects of PDIA3 on milk composition traits in Chinese Holstein cattle. It also revealed that certain SNPs and a haplotype block of the PDIA3 gene could be used as genetic markers for dairy cow selected breeding. Additionally, the study found that overexpression of PDIA3 gene positively regulated the synthesis and secretion of milk proteins.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yonghui Wang, Junyu Zhai, Chunhong Yang, Jingpeng Wang, Yan Sun, Yuhua Li, Zhihua Ju, Jingming Huang, Changfa Wang
Summary: This study identified novel single-nucleotide polymorphisms (SNPs) in the C3 gene and investigated their associations with serum C3 level, complement hemolytic activity, and milk production traits in Chinese cattle breeds. The results suggest that the C3 gene plays a role in resistance to bacterial infection and can be used as a molecular marker for complement activity and milk production traits.
Article
Agriculture, Dairy & Animal Science
Yujia Sun, Xinyi Wu, Yaoyao Ma, Dingding Liu, Xubin Lu, Tianqi Zhao, Zhangping Yang
Summary: This study performed an association analysis between polymorphisms of three candidate genes (ABCG2, CD44, SPP1) and milk production performance on Chinese Holstein cattle. The results showed that the different genotypes of these genes significantly affected milk production performance. ABCG2, CD44, and SPP1 genes could be selected for marker-assisted selection, which is of great significance for future precise molecular breeding.
Article
Agriculture, Dairy & Animal Science
Ankit Magotra, Y. C. Bangar, C. S. Patil, Pradeep Kamaldeep, Vikas Sindhu, Deepak Malik, Pradeep Chaudhary, Asha Rani Garg, Sandeep Kumar
Summary: The present study investigated the association between CXCR1 gene polymorphisms and clinical mastitis, reproductive disorders, and performance traits in Hardhenu cattle. A targeted SNP rs211042414 (C>T) in the CXCR1 gene was genotyped using PCR amplification and Bsa1 restriction enzyme digestion. Significant associations were found between the SNP and clinical mastitis occurrence. The CC genotype showed higher susceptibility to clinical mastitis and higher milk yields compared to CT and TT genotypes.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
Jale Metin Kiyici, Bilal Akyuz, Mahmut Kaliber, Korhan Arslan, Esma Gamze Aksel, Mehmet Ulas Cinar
Summary: This study found associations between GH-AluI and STAT5A-AvaI gene polymorphisms and milk electrical conductivity, but no significant associations were found between MYF5 gene polymorphism and the investigated traits. The results suggest that GH-AluI and STAT5A-AvaI polymorphisms could be useful for further studies aiming to improve mastitis resistance and milk quality traits of Holstein dairy cows.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
M. Sofia Ortega, Derek M. Bickhart, Kelsey N. Lockhart, Daniel J. Null, Jana L. Hutchison, Jennifer C. McClure, John B. Cole
Summary: The study presents a comprehensive framework for identification and validation of recessive alleles that represent genetic risk. Using Holstein haplotype 2 as an example, it demonstrates how the framework can be used to detect and confirm genetic defects that affect conception rate and stillbirths. The study also identifies specific variants associated with Holstein haplotype 2 and validates their function using knockout embryos.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Huimin Zhang, Ahmed A. Elolimy, Haji Akbar, Lam Phuoc Thanh, Zhangping Yang, Juan J. Loor
Summary: This study investigates the ruminal microbiome, milk fatty acid composition, and plasma concentrations of glucose, nonesterified fatty acids (NEFA), and beta-hydroxybutyrate in high-efficiency and low-efficiency dairy cows during early lactation. The findings suggest that high-efficiency cows have unique profiles of ruminal microbiota that are correlated with concentrations of milk fatty acids.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Genetics & Heredity
Yujia Sun, Yaoyao Ma, Tianqi Zhao, Mingxun Li, Yongjiang Mao, Zhangping Yang
Summary: In this study, we investigated the transcriptional and epigenetic mechanisms of meat traits in Qinchuan cattle and found that the CFL1 gene plays a crucial role in the development and differentiation of bovine primary myoblasts. Our findings have significant implications for improving meat production and quality in Qinchuan cattle.
Article
Agriculture, Dairy & Animal Science
Cheng Tang, Yan Liang, Jiahe Guo, Mengqi Wang, Mingxun Li, Huimin Zhang, Abdelaziz Adam Idriss Arbab, Niel A. Karrow, Zhangping Yang, Yongjiang Mao
Summary: Heat stress during late gestation can negatively affect the growth, metabolism, and immune function of calves, leading to decreased lactation performance after weaning.
Article
Biochemistry & Molecular Biology
Yan Liang, Qisong Gao, Haiyang Wang, Mengling Guo, Abdelaziz Adam Idriss Arbab, Mudasir Nazar, Mingxun Li, Zhangping Yang, Niel A. Karrow, Yongjiang Mao
Summary: In this study, circRNAs from Holstein cow mammary tissues during early lactation and non-lactation were identified and compared. The study revealed a large number of circRNAs and identified significantly differentially expressed circRNAs between peak lactation and non-lactating cows. GO analysis showed the involvement of circRNAs in immune response and triglyceride transport, while pathway analysis indicated their role in cytokine signaling and fatty acid biosynthesis. The findings provide valuable information for studying circRNA functions, mechanisms, and miRNA genes related to lactation in Holstein cows.
Article
Biochemistry & Molecular Biology
Tianle Xu, Run Liu, Hao Zhu, Yu Zhou, Tianxu Pei, Zhangping Yang
Summary: In this study, it was found that EGCG can alleviate hepatic inflammation and oxidative stress induced by LPS. EGCG exerts its hepatoprotective effects by inhibiting the MAPK/NF-kappa B signaling pathway and activating the Nrf2 cascade.
Article
Agronomy
Run Liu, Hao Zhu, Jingwen Zhao, Xinyue Wu, Xubin Lu, Tianle Xu, Zhangping Yang
Summary: In this study, it was found that Lycium barbarum polysaccharide (LBP) can alleviate Escherichia coli-induced mastitis in dairy cows by activating the suppressor of cytokine signaling-3 (SOCS3) pathway and inhibiting MAPK signaling. LBP pretreatment inhibited inflammation and oxidative stress, and decreased the expression of pro-inflammatory factors.
Review
Cell Biology
Yuanyuan Zhang, Zhichen Tian, Haibo Ye, Xiaomei Sun, Huiming Zhang, Yujia Sun, Yongjiang Mao, Zhangping Yang, Mingxun Li
Summary: This review provides an overview of the structure and biosynthetic mechanism of circRNAs and discusses the circRNAs related to adipocyte formation. By studying the genetic regulatory mechanisms of these circRNAs, a better understanding of the gene regulation process in adipocyte metabolism can be achieved.
CELL DEATH DISCOVERY
(2022)
Article
Agriculture, Dairy & Animal Science
Mudasir Nazar, Ismail Mohamed Abdalla, Zhi Chen, Numan Ullah, Yan Liang, Shuangfeng Chu, Tianle Xu, Yongjiang Mao, Zhangping Yang, Xubin Lu
Summary: Udder conformation traits are economically important for dairy animals, and understanding their genetic architecture is crucial for genetic improvement and long-term selection in Holstein Friesian cattle. By conducting a GWAS on udder traits in Chinese Holstein cattle, several significant SNPs and candidate genes associated with udder structure traits were identified. These findings provide valuable information for improving the genetic information, health, longevity, and production of dairy cattle.
Article
Agriculture, Dairy & Animal Science
Yujia Sun, Xinyi Wu, Yaoyao Ma, Dingding Liu, Xubin Lu, Tianqi Zhao, Zhangping Yang
Summary: This study performed an association analysis between polymorphisms of three candidate genes (ABCG2, CD44, SPP1) and milk production performance on Chinese Holstein cattle. The results showed that the different genotypes of these genes significantly affected milk production performance. ABCG2, CD44, and SPP1 genes could be selected for marker-assisted selection, which is of great significance for future precise molecular breeding.
Article
Agronomy
Xubin Lu, Hui Jiang, Abdelaziz Adam Idriss Arbab, Bo Wang, Dingding Liu, Ismail Mohamed Abdalla, Tianle Xu, Yujia Sun, Zongping Liu, Zhangping Yang
Summary: This study analyzed the genetic characteristics of somatic cell score (SCS) in Chinese Holstein cattle and identified candidate genes and biological processes related to cow mastitis. The heritability of SCS varied between 0.07 and 0.24, decreasing with parity. Six significant SNPs were found, including one in the CD44 gene that may alter protein activity. Additionally, 32 genes near significant SNPs were identified and involved in various pathways and processes related to mastitis development. Nine candidate genes (PKD2, KCNAB1, SLC35A4, SPP1, IBSP, CD14, CD44, MAPK10, and ABCG2) were highlighted for potential roles in mastitis. These findings can contribute to molecular breeding efforts and reducing mastitis incidence in Chinese Holstein cattle.
Article
Agronomy
Tianle Xu, Wendi Cao, Yicai Huang, Jingwen Zhao, Xinyue Wu, Zhangping Yang
Summary: Bovine mastitis is a common disease in dairy farms, leading to reduced milk production and quality. Pathogenic bacteria enter the mammary gland through contagious or environmental routes. The study investigated the epidemiological characteristics and drug resistance of bovine mastitis pathogens in northern Jiangsu. Findings showed that E. coli was the most prevalent bacteria, followed by K. pneumoniae and Staphylococcus spp. Susceptibility testing revealed that gentamicin and tetracycline were effective against E. coli, while beta-lactams showed resistance. Several drug resistance genes were detected. This study provides important information on the distribution and antimicrobial resistance of bovine mastitis pathogens.
Article
Biotechnology & Applied Microbiology
Yujia Sun, Tianqi Zhao, Yaoyao Ma, Xinyi Wu, Yongjiang Mao, Zhangping Yang, Hong Chen
Summary: The CFL2 gene plays an important role in meat quality, and its expression and DNA methylation levels are related to cattle age. Cell experiments and adenovirus vector experiments have demonstrated that the CFL2 gene promotes the differentiation of bovine myoblasts by regulating muscle marker genes.
BIOENGINEERING-BASEL
(2022)
Article
Genetics & Heredity
Shuangfeng Chu, Yi Yang, Mudasir Nazar, Zhi Chen, Zhangping Yang
Summary: In this study, miR-497 was found to play an important role in fat metabolism by analyzing miRNA expression profiles in mammary gland tissues of cows at different lactation stages. MiR-497 mimics inhibited fat metabolism, while miR-497 knockdown promoted fat metabolism in bovine mammary epithelial cells. Further research revealed that miR-497 could down-regulate the expression of LATS1, which in turn influenced fatty acid, triacylglycerol, and cholesterol concentrations in cells.
Review
Genetics & Heredity
Haoyue Guo, Jianyuan Li, Yuhao Wang, Xiang Cao, Xiaoyang Lv, Zhangping Yang, Zhi Chen
Summary: Lactation initiation refers to the transition of the mammary organ from a non-lactating state to a lactating state, involving cytological changes in the mammary epithelium. It is regulated by various factors and can be stimulated by specific stimuli before and after parturition. This review provides an overview of recent progress in understanding the key factors involved in lactation initiation and mammary gland development, supporting further research in this area.
Article
Agriculture, Dairy & Animal Science
Ismail Mohamed Abdalla, Jiang Hui, Mudasir Nazar, Abdelaziz Adam Idriss Arbab, Tianle Xu, Shaima Mohamed Nasr Abdu, Yongjiang Mao, Zhangping Yang, Xubin Lu
Summary: Body size is an important economic trait for dairy cows, and understanding the genetic basis of inter-individual variation in body size can accelerate genetic improvement programs. In this genome-wide association study on Chinese Holstein cattle, several genes and functional pathways related to body size traits were identified. These findings can contribute to breeding programs and genomic selection efforts.
