Article
Biochemistry & Molecular Biology
Maria Jose Gomez-Torres, Javier Luna-Romero, Pedro Jose Fernandez-Colom, Jon Aizpurua, Manuel Aviles, Alejandro Romero
Summary: Nuclear vacuoles are specific structures present on the head of the human sperm. This study aimed to define the incidence, position, morphology, and molecular content of the human sperm vacuoles. Results showed that approximately 50% of analyzed human sperm cells contained vacuoles, mainly located in the anterior head region. Furthermore, it was discovered that these vacuoles were invaginations of the nuclear envelope containing cytoskeletal proteins and a cytoplasmic enzyme.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Paula Saez-Espinosa, Cristina Franco-Esclapez, Laura Robles-Gomez, Willian T. A. F. Silva, Alejandro Romero, Simone Immler, Maria Jose Gomez-Torres
Summary: The study revealed significant morphological and ultrastructural changes in zebrafish spermatozoa after motility activation, including distortion of the head and damage to the midpieces.
Review
Medicine, General & Internal
Caroline Kang, Nahid Punjani, Peter N. Schlegel
Summary: Non-obstructive azoospermia (NOA) is the most severe form of infertility, caused by spermatogenic dysfunction leading to lack of sperm in the ejaculate. Men with this condition should be evaluated before treatment to determine underlying causes. Despite the various etiologies, most NOA patients are suitable candidates for surgical sperm retrieval and ICSI treatment.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Lea Simone Becker, Mohammad A. A. Al Smadi, Markus Raeschle, Shusruto Rishik, Hashim Abdul-Khaliq, Eckart Meese, Masood Abu-Halima
Summary: Using liquid chromatography-mass spectrometry and bioinformatics analyses, we investigated the proteomic landscape of sperm from subfertile men. We identified 4412 proteins in human sperm and found differential abundance of 1336 proteins. These abundance changes are likely to impact sperm function and can be used for future diagnosis of male subfertility.
Article
Entomology
Glenda Dias, David Mercati, Paulo Henrique Rezende, Jose Lino-Neto, Pietro Paolo Fanciulli, Pietro Lupetti, Romano Dallai
Summary: Tenebrionoidea, with over 30,000 described species and 30 recognized families, is a superfamily with challenging taxonomy. By studying the sperm ultrastructure, a better understanding of the phylogenetic relationships of basal families within the group can be achieved.
Article
Cell Biology
Lin Chen, Junli Song, Jinglei Zhang, Zicong Luo, Xuren Chen, Canquan Zhou, Xiaoting Shen
Summary: This study found that SPACA4 is a protein expressed during sperm development, and its localization changes intracellularly, suggesting its potential role in human fertilization. However, there is no significant association between SPACA4 protein levels and fertilization or cleavage rates. Larger clinical trials are needed to evaluate the potential use of sperm SPACA4 protein levels in predicting fertilization potential.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Obstetrics & Gynecology
Haruka Matsukuma, Yuka Kobayashi, Shintaro Oka, Fumiaki Higashijima, Kazuhiro Kimura, Erika Yoshihara, Noriaki Sasai, Koji Shiraishi
Summary: This study reveals the crucial role of Prom1 gene in spermatogenesis. It maintains normal spermatogenesis by promoting cell proliferation and survival, and it is also involved in sperm motility and fertilization potential. Furthermore, the loss of Prom1 gene leads to abnormal sperm morphology and decreased motility, but the underlying mechanism remains to be elucidated.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2023)
Article
Anatomy & Morphology
Jonathan R. Cowart, Danielle M. Collins, Daniel L. Stanton, Gerhard van Der Horst, Iskande V. Larkin
Summary: This study provides new morphometric and ultrastructural data for Florida manatee sperm, revealing unique features and potential effects of sexual selective pressures. The findings offer insights for further research on reproductive physiology and sperm competition in this species.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2022)
Article
Entomology
Camila Folly, Italo Salvatore de Castro Pecci-Maddalena, Cristiano Lopes-Andrade, E. Jose Lino-Neto
Summary: The study provides a detailed anatomical and histological investigation of the reproductive system and sperm structure of Ceracis cornifer Mellie (Ciidae). It reveals unique characteristics such as the growth of a single oocyte at a time in females and antiparallel organization of sperm within cysts in males, as well as sperm dimorphism in C. cornifer, which is described for the first time in a Tenebrionoidea taxon.
ARTHROPOD STRUCTURE & DEVELOPMENT
(2021)
Article
Fisheries
N. Sukkhee, T. Mitparian, T. Kanjanarakha, S. Senarat, W. Jiraungkoorskul, N. Kangwanrangsan, G. Kaneko, J. Kettratad
Summary: The study described the spermatogenesis of benthic toadfish from Estuarine Pranburi River, Thailand at the ultrastructural level. The spermatogenic cells of A. grunniens were classified into five stages based on nuclear and cytoplasmic characteristics, showing the development from spermatogonium to spermatozoon. The observation of Batrachoididae spermatozoa ultrastructure provides insights into their reproductive biology.
JOURNAL OF ICHTHYOLOGY
(2021)
Article
Microscopy
Romano Dallai, David Mercati, Pietro Paolo Fanciulli, Pietro Lupetti
Summary: The sperm cells of the diving beetle Deronectes moestus incospectus form bundles of 64 units through sperm conjugation. These bundles are formed in the testes and can be found in the anterior region of the deferent ducts. The sperm bundles are surrounded by extracellular material secreted by the epithelial cells of the ducts, which covers the nuclei and initial region of the flagella.
Article
Fisheries
Victor Mauricio Medina-Robles, Leydy Yasmin Sandoval-Vargas, Roger Oswaldo Suarez-Martinez, Edwin Gomez-Ramirez, Diana Nataly Guaje-Ramirez, Pablo Emilio Cruz-Casallas
Summary: Little attention has been given to the effects of long-term cryopreservation on sperm quality in Piaractus orinoquensis. This study examined the effects of cryopreservation for different periods of time on various sperm parameters in this species. The results showed a decrease in sperm motility, viability, DNA integrity, and ATP content after 6 months of cryopreservation. The total antioxidant capacity did not significantly differ between fresh and cryopreserved sperm. Cryopreserved samples also exhibited an increase in sperm abnormalities compared to fresh sperm, with short tail being the most common defect. Ultrastructural analysis revealed damage to the plasma membrane, mitochondria, nucleus, and mid-region of post-thaw spermatozoa.
AQUACULTURE REPORTS
(2023)
Article
Multidisciplinary Sciences
Rudiger Bieler, Timothy M. Collins, Rosemary Golding, Camila Granados-Cifuentes, John M. Healy, Timothy A. Rawlings, Petra Sierwald
Summary: Vermetid worm-snails are a common marine mollusk species found in warm nearshore and coral reef environments. This study focuses on two special groups of Vermetid worm-snails, Thylacodes and Cayo, and examines their morphology, behavior, and genetic characteristics. The research reveals unique differences in these species, including the loss of opercula, polychromatic head-foot coloration, and the evolution of predator avoidance mechanisms.
Article
Toxicology
Hidenobu Miyaso, Kenta Nagahori, Kaiya Takano, Takuya Omotehara, Shinichi Kawata, Zhong-Lian Li, Miyuki Kuramasu, Xi Wu, Yuki Ogawa, Masahiro Itoh
Summary: Neonatal maternal separation in male mice leads to reduction in testicular weight, epididymal sperm number, and Sertoli cell numbers, as well as various morphological changes in the testes. Decreased serum testosterone levels were observed after separation, indicating a negative impact on spermatogenesis activity in adulthood.
TOXICOLOGY MECHANISMS AND METHODS
(2021)
Article
Obstetrics & Gynecology
Lars Bjorndahl, Jackson Kirkman Brown
Summary: The principles of basic semen investigation are crucial for obtaining reliable and internationally comparable results. However, many studies and practices in reproductive medicine fail to adhere to these principles, leading to a lack of comparable data and accuracy. To address this issue, the sixth edition of the WHO manual introduces revised methods and a complementary formal standard from the International Standards Organization. The new edition also reintroduces the four-category distinction of sperm motility and emphasizes the importance of semen examination for assessing male reproductive functions and overall male health.
FERTILITY AND STERILITY
(2022)