Journal
ANIMAL
Volume 8, Issue 11, Pages 1867-1872Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1751731114001694
Keywords
miR-27a; porcine myostatin; 3 ' UTR; cloning; porcine myoblast proliferation
Funding
- National Natural Science Foundation of China [31272459]
- National Basic Research Program of China [2012CB124701]
- Sichuan Youth Science and Technology Foundation [2012JQ0049]
- Specific Research Supporting Program for Academic Sustentation Research Team in Sichuan Agricultural University
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MicroRNAs are endogenous similar to 22nt RNAs that negatively regulate gene expression at the posttranscriptional level via binding to the 3'-untranslated region (3'UTR) of target mRNAs. The microRNA miR-27a was reported to depress the expression of myostatin, a critical inhibitor of skeletal myogenesis, by binding to its 3'UTR in mouse. In this study, we cloned the full-length 3'UTR of porcine myostatin by rapid amplification of 3'-cDNA ends (3'-RACE) and demonstrated that the 3'UTR of porcine myostatin is targeted by miR-27a. The phenomenon that the level of myostatin inversely correlated with miR-27a was observed in fat and heart of pigs and also in proliferating porcine myoblasts. Besides, overexpression of miR-27a in porcine myoblasts promoted cell proliferation by reducing the expression of myostatin. Our data suggest that miR-27a positive regulates porcine myoblast proliferation via targeting myostatin.
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