Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 53, Issue 44, Pages 11787-11792Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201406029
Keywords
C. elegans; cell metabolism; lipids; stimulated Raman scattering; vibrational spectroscopy
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Funding
- NIH Office of Research Infrastructure Programs [P40 OD010440]
- National Institutes of Health [GM104681]
- William Randolph Hearst Foundation [AG031237]
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Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans.
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