Review
Biochemistry & Molecular Biology
Kangran Zhang, Yezhong Tang, Qin Chen, Yang Liu
Summary: This review discusses the regular process of using phage display technology to screen therapeutic peptides and the peptides that have been screened for their anti-inflammatory properties in recent years. The article describes how these peptides were screened and their in vitro and in vivo effects. It also explores the current challenges and future outlook of using phage display to obtain anti-inflammatory therapeutic peptides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Golnaz Kamalinia, Brian J. Grindel, Terry T. Takahashi, Steven W. Millward, Richard W. Roberts
Summary: mRNA display is a powerful platform for directed evolution of proteins and peptides, linking phenotype and genotype through puromycin. Iterative selection cycles lead to peptides with desired functional levels. This review discusses the development and broad application of mRNA display technology in selecting novel peptides and proteins.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Nanoscience & Nanotechnology
Ji Hong Kim, Hye-Seon Jeong, Jaehyeon Hwang, Dae-Hyuk Kweon, Chang-Hyung Choi, Jong Pil Park
Summary: A sensor based on affinity peptide-immobilized hydrogel microspheres has been developed for the selective detection of influenza A virus. The sensor shows rapid and accurate detection capability, which is crucial for the effective control of pandemic flu outbreaks.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
David M. Boragine, Wanzhi Huang, Lynn H. Su, Timothy Palzkill
Summary: Peptides that bind and disrupt protein-protein interaction interfaces can be identified through systematic peptide library construction, affinity selection, and deep sequencing.
Article
Chemistry, Analytical
Yanbo Wang, Mingyang Wang, Haipeng Yu, Ge Wang, Pengxin Ma, Shuang Pang, Yiming Jiao, Aihua Liu
Summary: PSA is an important index for prostate cancer diagnosis and treatment. In this study, a biopanning method for PSA was established using a pIII phage display library. Two phage monoclonal antibodies specific to PSA were obtained, and an ELISA system was constructed for detecting tPSA with high accuracy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
David N. Philpott, Surath Gomis, Hansen Wang, Randy Atwal, Abdellali Kelil, Tanja Sack, Brandon Morningstar, Chris Burnie, Edward H. Sargent, Stephane Angers, Sachdev Sidhu, Shana O. Kelley
Summary: The rapid on-cell phage display platform presented in this study accelerates the development of high-performance human antibodies by mimicking the complex in vivo binding environment, improving screening efficiency and accuracy.
ACS CENTRAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hawk Leong Yuen, Szn Yi Chan, Yi En Ding, Suxing Lim, Gim Cheong Tan, Chiew Ling Kho
Summary: This study aimed to develop a potent antibacterial peptide (ABP) with minimal toxicity through phage display selection and computer-assisted modification. A peptide called PAM-5 with enhanced antibacterial features was obtained by modifying the peptide selected for its high affinity to Pseudomonas aeruginosa cell surface using an online peptide database. PAM-5 showed activity against various Gram-negative and selected Gram-positive bacteria, and was stable in human plasma without toxic effects on human cells and erythrocytes.
Review
Microbiology
Weronika Jaroszewicz, Joanna Morcinek-Orlowska, Karolina Pierzynowska, Lidia Gaffke, Grzegorz Wegrzyn
Summary: Phage display technology is a widely applicable method in biotechnology, recognized by the Nobel Prize. This article provides an overview of the technology, including comparisons of different display systems, their advantages and disadvantages, and examples of applications in various fields. It also discusses other peptide display technologies and highlights the ongoing development and improvements in these methods, offering new solutions in the future.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Sebastian Dengler, Ryan T. Howard, Vasily Morozov, Christos Tsiamantas, Wei-En Huang, Zhiwei Liu, Christopher Dobrzanski, Vojislava Pophristic, Sophie Brameyer, Celine Douat, Hiroaki Suga, Ivan Huc
Summary: Expanding the chemical diversity of peptide macrocycle libraries through the inclusion of a large aromatic helical foldamer has been implemented. A hybrid macrocyclic nanomolar binder to the C-lobe of the E6AP HECT domain was selected, and its crystal structure and molecular dynamics simulations revealed an intriguing reciprocal helical folding of the peptide and foldamer. The results represent a significant extension in the chemical space amenable to display selection and highlight the potential benefits of incorporating an aromatic foldamer into a peptide macrocycle for protein recognition.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Sarah Barakat, Melike Berksoz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman
Summary: Camelidae derived single-domain antibodies, known as nanobodies, are small antibody fragments with full antigen-binding capacity. They have gained popularity due to their small size, high specificity, strong affinity, excellent stability, and modularity. Nanobodies are now widely used as molecular probes in the field of molecular imaging, allowing the detection and tracking of various targets. This review provides an overview of their applications and discusses current techniques for their generation and delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Radoslaw W. Piast, Rafal M. Wieczorek, Nicola Marzec, Maciej Garstka, Aleksandra Misicka
Summary: Chondrocalcinosis is a metabolic disease caused by calcium pyrophosphate dihydrate crystals in the synovial fluid. The study aimed to investigate whether short peptides could dissolve these crystals, with one peptide named R25 showing hydrolytic activity towards the crystals, dependent on Mg2+ and working against soluble pyrophosphate species.
Article
Virology
Marwa Arbi, Imen Larbi, Jihen Nsiri, Imen El Behi, Ahmed Rejeb, Khaled Miled, Adeljelil Ghram, Mehdi Houimel
Summary: This study identified new antiviral peptides that can inhibit the infectivity of H9N2 virus by screening a random linear hexapeptide library. Sixteen different phage-peptides were found to specifically bind to H9N2 virus, and two selected peptides (LSRMPK and FAPRWR) showed antiviral activity in both in ovo and in vivo experiments. These peptides prevent infection by inhibiting the attachment of the H9N2 virus to the cellular receptor.
Article
Multidisciplinary Sciences
Ruiqi Huang, Gabrielle Warner Jenkins, Yunjeong Kim, Robyn L. Stanfield, Amrinder Singh, Maria Martinez-Yamout, Gerard J. Kroon, Jonathan L. Torres, Abigail M. Jackson, Abigail Kelley, Namir Shaabani, Baisen Zeng, Michael Bacica, Wen Chen, Christopher Warner, Jasmina Radoicic, Joongho Joh, Krishani Dinali Perera, Huldah Sang, Tae Kim, Jianxiu Yao, Fangzhu Zhao, Devin Sokd, Dennis R. Burton, Jeff Allen, William Harriman, Waithaka Mwangi, Donghoon Chung, John R. Teijaro, Andrew B. Ward, H. Jane Dyson, Peter E. Wright, Ian A. Wilson, Kyeong-Ok Chang, Duncan Mcgregor, Vaughn V. Smider
Summary: Antibodies derived from cows have the potential to be a new class of therapeutics, capable of neutralizing various SARS-CoV-2 variants and providing protection against disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Pharmacology & Pharmacy
Ali Akbar Alizadeh, Michael B. Morris, W. Bret Church, Shadi Yaqoubi, Siavoush Dastmalchi
Summary: TNFa plays a crucial role in physiological processes, but excessive levels can contribute to inflammatory and autoimmune diseases. Therefore, researchers have focused on developing strategies to counteract aberrant TNFa concentrations. Promising approaches include destabilizing the active form of TNFa and blocking its interaction with TNFa receptor type I. The use of phage-display technology has been successful in identifying peptides and antibodies that inhibit TNFa activity or prevent its binding to its receptor.
DRUG DISCOVERY TODAY
(2022)
Review
Chemistry, Analytical
Ping Xu, Subhadeep Ghosh, Anam Rana Gul, Jigna R. Bhamore, Jong Pil Park, Tae Jung Park
Summary: Biosensors have seen rapid growth in the field of biological detection and biomedical diagnosis, with displayed peptides from phage display libraries being recognized as a promising alternative to antibodies. The advantages of displayed peptides include high affinity, low cost, ease of design and modification, mass production, and increased resistance to complex environments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Samyukta Ravishankar, Seigo Suzuki, Toshiki Sawada, Sierin Lim, Takeshi Serizawa
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2020)
Article
Biochemistry & Molecular Biology
Yuuki Hata, Tomoya Kojima, Tohru Maeda, Toshiki Sawada, Takeshi Serizawa
MACROMOLECULAR BIOSCIENCE
(2020)
Article
Engineering, Chemical
Toshiki Sawada, Hiroki Fukuta, Takeshi Serizawa
Article
Chemistry, Multidisciplinary
Iljae Min, Yusuke Tamaki, Osamu Ishitani, Takeshi Serizawa, Yoshihiro Ito, Takanori Uzawa
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2020)
Article
Polymer Science
Iljae Min, Takanori Uzawa, Takeshi Serizawa, Yoshihiro Ito
Summary: Using PCR-mediated recombination, researchers prepared single RNA chains containing bifunctional RNA sequences for substrate binding and phosphorescent signaling. Structural and functional analyses confirmed that the conjugated RNAs maintained the binding abilities and functions of the original aptamers, making them suitable as phosphorescent RNA probes for protein cognates. This one-step drawing strategy shows promise as a biological method for generating phosphorescent RNA probes without loss of function from the original aptamers.
Article
Virology
Toshiki Sawada, Rina Oyama, Michihiro Tanaka, Takeshi Serizawa
Article
Chemistry, Physical
Yuuki Hata, Shohei Yoneda, Shoki Tanaka, Toshiki Sawada, Takeshi Serizawa
Summary: The study demonstrates that using nonionic crystalline surfactants can form a crystalline monolayer at water-organic solvent interfaces, allowing arrested shape changes of liquid-liquid interfaces. This enables the successful creation of irregular-shaped liquid droplets under various solution conditions.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Misaki Hanamura, Toshiki Sawada, Takeshi Serizawa
Summary: This study demonstrates a new strategy for noncovalently modifying cellulose paper through heterogeneous nucleation based self-assembly of functional cellulose oligomers, achieving efficient and stable modification. The chemically reactive all-cellulose paper prepared in this way showed successful detection of anti-biotin immunoglobulin G with high signal-to-background ratio, showcasing its potential for constructing various sustainable paper-based devices.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Review
Chemistry, Multidisciplinary
Yuuki Hata, Takeshi Serizawa
Summary: Dynamic properties are not essential to molecular assemblies, and statically assembled molecular gels also have unique applications.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2021)
Article
Polymer Science
Kai Sugiura, Toshiki Sawada, Hiroshi Tanaka, Takeshi Serizawa
Summary: The use of bifunctional oligomeric primers has been shown to propagate cellulose oligosaccharide chains from both ends, providing a new method for the synthesis of unique cellulose block co-oligomers. This finding is significant for the CDP-catalyzed synthesis of various cellulose block co-oligomers and has the potential to produce novel cellulose materials at different scales.
Article
Polymer Science
Tomio Iwasaki, Masashi Maruyama, Tatsuya Niwa, Toshiki Sawada, Takeshi Serizawa
Summary: By utilizing materials informatics technology and molecular dynamics simulation, peptides with strong binding affinities for PMMA resin were designed to cover the resin surface, aiming to create eco-friendly and biocompatible biomaterials. By analyzing simulation data, it was found that three peptides containing arginine, tryptophan, and proline exhibited strong binding affinities to PMMA resin components. Experimental verification confirmed the strong binding affinities of these peptides.
Article
Nanoscience & Nanotechnology
Seigo Suzuki, Toshiki Sawada, Takeshi Serizawa
Summary: This study introduces a simple method for identifying synthetic polymers dissolved in water using peptide-based molecular sensors. By utilizing machine learning to process multiple fluorescence signals from the sensor interacting with the polymers, successful classification of different polymer species or different mixture ratios was achieved.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Physical
Takeshi Serizawa, Shoki Tanaka, Toshiki Sawada
Summary: The control of molecular arrangement in synthetic assemblies of biorelated molecules is an attractive research focus, with researchers investigating parameters such as temperature, salt concentration, salt species, and solvent species to influence molecular arrangement. Experimental results showed that factors like temperature, inorganic salt concentration and species, and solvent species strongly affected the molecular arrangement in cellulose-based synthetic assemblies.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Polymer Science
Yuta Sakurai, Toshiki Sawada, Takeshi Serizawa
Summary: The modulation of intermolecular interactions between cellulose oligomers by protein denaturants under suitable synthesis conditions can lead to the production of oligomers with different crystal structures and higher degrees of polymerization, providing important fundamental knowledge for the development of novel cellulose materials.
Review
Materials Science, Biomaterials
Yuuki Hata, Takeshi Serizawa
Summary: Biomolecules such as nucleic acids, peptides, and lipids have been utilized as building blocks for creating advanced nanostructured materials in vitro, but directing cellulose to controllably assemble into ordered nanostructures proves challenging due to its lack of structural diversity. However, cellulose assemblies exhibit outstanding robustness and inertness, making them attractive for constructing versatile materials, especially in biomedical applications. Gaining control over cellulose assembly presents a promising route for producing green materials with tailor-made nanostructures, given that cellulose is the most abundant biopolymer on Earth.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
