Journal
INTERNATIONAL JOURNAL OF NEUROSCIENCE
Volume 126, Issue 4, Pages 326-334Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/00207454.2014.994209
Keywords
epilepsy; hypoxia-inducible factor-1 alpha; miRNA-199; hypoxia; recurrent seizures
Categories
Funding
- National Natural Science Foundation of China [81071039]
Ask authors/readers for more resources
Objectives: During the last decade, experimental evidence has demonstrated an important role of hypoxia, which leads to neuronal cell death and angiogenesis, in the mechanisms of seizure precipitation and recurrence. MicroRNA-199 targets hypoxia-inducible factor-1alpha (HIF-1), which has recently been implicated in the pathophysiology of the hypoxic state and brain injury. However, little is known about the roles of MicroRNA-199 and HIF-1 in the human epileptogenic process.Design and methods: In this study, we investigated the expression of miR-199a-5p, miR-199b-5p and HIF-1 using real-time PCR, immunohistochemistry and western blots in the temporal neocortex of twenty four patients with intractable epilepsy and twelve control subjects.Results: Compared with the control group, the expression of miR-199a-5p and miR-199b-5p was significantly lower in epileptic brain tissues (p < 0.05). The levels of HIF-1 mRNA and protein were highly up-regulated in epileptic brain tissues compared with those of control subjects (p < 0.05).Conclusion: These data suggest that the abnormal expression of miR-199 and HIF-1 in epileptic brain tissue may be involved in the pathophysiology of human epilepsy and that the expression of HIF-1 may be regulated by miR-199. These findings may provide new insights into the treatment of epilepsy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available