Protective effect of beta-carotene against titanium dioxide nanoparticles induced apoptosis in mouse testicular tissue

In this study, the effects of beta-carotene (BC) on testicular germ cell apoptosis arising from titanium dioxide nanoparticles (NTiO2) have been evaluated. In NTiO2-treated mice, expression of apoptotic related genes including Bid, FasL, caspase-3 and p38MAPK was significantly increased. Measurement apoptosis using TUNEL method showed significant increase in apoptotic index of germ cells in NTiO2-treated mice (P<0.05). TUNEL assessments showed that the increase of apoptotic index of testicular germ cells in NTiO2-treated mice was reversed by BC. Beta-carotene pre-treatment could also effectively attenuate the expression of apoptotic related genes. The application of BC may serve as a beneficial medication to protect germ cells against apoptosis induced by nanoparticles and be helpful for male fertility.