Article
Multidisciplinary Sciences
Zhuoran Ma, Feifei Wang, Weizhi Wang, Yeteng Zhong, Hongjie Dai
Summary: This study utilized artificial neural networks to transform fluorescence images in the shorter-wavelength NIR window to images resembling NIR-IIb window, achieving high signal-to-background ratio in vivo lymph node imaging with human-approved molecular probes. Translation of PD-L1 or EGFR imaging greatly enhanced tumor-to-normal tissue ratio and improved tumor margin localization, showcasing the potential of deep learning in enhancing noninvasive NIR imaging and microscopy. Deep learning equipped NIR imaging could facilitate basic biomedical research and clinical diagnostics and imaging-guided surgery.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Jingke Yao, Jose Lifante, Paloma Rodriguez-Sevilla, Maria de la Fuente-fernandez, Francisco Sanz-Rodriguez, Dirk H. Ortgies, Oscar Gomez Calderon, Sonia Melle, Erving Ximendes, Daniel Jaque, Riccardo Marin
Summary: The study introduces a novel AgInSe2 nanoparticle-based nanocapsule contrast agent for fluorescence imaging, showing great potential for high-resolution, whole-body in vivo imaging with deeper tissue penetration in the second biological window.
Article
Engineering, Biomedical
Gaizhen Kuang, Hongtong Lu, Shasha He, Hejian Xiong, Jie Yu, Qingfei Zhang, Yubin Huang
Summary: Multifunctional nanoparticles combining multiple imaging modes and NIR light-activated drug/gene delivery have great potential in cancer therapy, displaying excellent therapeutic efficacy both in vitro and in vivo.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Multidisciplinary Sciences
Chae Moon Hong, Prakash Gangadaran, Ji Min Oh, Ramya Lakshmi Rajendran, Arunnehru Gopal, Liya Zhu, Byeong-Cheol Ahn
Summary: This study developed a nuclear imaging method for tracking EVs derived from cell lines, showing that radioiodine-labeled EVs had good biodistribution. The radioiodine labeling did not significantly affect the morphology and properties of EVs.
SCIENTIFIC REPORTS
(2021)
Review
Chemistry, Multidisciplinary
Chavee Laomeephol, Sudartip Areecheewakul, Supannikar Tawinwung, Koramit Suppipat, Preedakorn Chunhacha, Nuno M. Neves, Jittima Amie Luckanagul
Summary: Chimeric antigen receptor (CAR) T cell therapy has shown remarkable efficacy in cancer treatment, but its complex manufacturing process limits its application. In vivo generation of CAR T cells provides an alternative immunotherapy. Hyaluronic acid (HA) is a natural biopolymer that can be used for gene delivery due to its inherent properties. This review discusses the potential of HA in delivering CAR constructs and provides insights into rational design of HA-based systems for efficient CAR delivery to circulating T cells.
Review
Chemistry, Multidisciplinary
Jia Yu, Guoyu Jiang, Jianguo Wang
Summary: In vivo fluorescence imaging has gained attention for its excellent biosafety, high sensitivity, real-time monitoring ability, and non-invasiveness. Aggregation-induced emission luminogens (AIEgens) with near-infrared (NIR) absorption and emission wavelengths are ideal for this imaging due to their large Stokes shift, high brightness, and photostability. This review summarizes the strategies for constructing high-performance NIR AIEgens and highlights the encapsulation strategies to increase their water solubility and biocompatibility. The challenges and prospects of fabricating NIR AIEgens for in vivo fluorescence imaging are also discussed.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Engineering, Biomedical
Adi Hendler-Neumark, Verena Wulf, Gili Bisker
Summary: Caenorhabditis elegans (C. elegans) nematodes are commonly used as model organisms for genetic research, and in this study, near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) were effectively utilized for in vivo imaging to overcome autofluorescence interference. This method allowed for spectral separation between SWCNT NIR fluorescence and worm gut granule autofluorescence, enabling accurate tracking of physiological processes within the worms.
MATERIALS TODAY BIO
(2021)
Article
Chemistry, Multidisciplinary
Jonathan O. Martinez, Michael Evangelopoulos, Ava A. Brozovich, Guillermo Bauza, Roberto Molinaro, Claudia Corbo, Xuewu Liu, Francesca Taraballi, Ennio Tasciotti
Summary: The study introduces a versatile multicomponent delivery platform that combines MSC tropism with MSV-mediated payload delivery, demonstrating increased drug release and reduced inflammation, resulting in improved outcomes in experimental models.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Lu-Lu Chen, Liang Zhao, Zhi-Gang Wang, Shu-Lin Liu, Dai-Wen Pang
Summary: NIR-II imaging breakthrough imaging limitations in deep tissues, while NIR-II quantum dots exhibit excellent optical properties and have become promising fluorescent biomarkers in in vivo fluorescence imaging.
Article
Biotechnology & Applied Microbiology
Soon Hee Kim, Jin Seon Kwon, Jae Gu Cho, Kate G. Park, Tae Hyeon Lim, Moon Suk Kim, Hak Soo Choi, Chan Hum Park, Sang Jin Lee
Summary: Cell-based tissue engineering strategies have been widely established, but the contributions of transplanted cells to tissue regeneration are not well understood. A near-infrared fluorescence imaging system was optimized to non-invasively monitor transplanted cell-based tissue constructs. Mesenchymal stem cells labeled with a lipophilic pentamethine indocyanine showed high printability and cell viability in 3D constructs, allowing for successful tissue formation in a rat model without specific staining. This NIR-based imaging system has the potential to validate cell fate in cell-based tissue engineering applications.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Giorgia Zambito, Gunja Mishra, Christopher Schliehe, Laura Mezzanotte
Summary: Melanoma is an aggressive type of skin cancer with a poor prognosis. Early detection is crucial for effective therapy. Recent research has shown that the interaction between melanoma cells and the immune system can aid in the development of efficient treatment strategies. This study used engineered macrophages as cell-based sensors to detect metastatic melanoma, demonstrating the potential of using optical imaging technologies to track circulating macrophages for non-invasive detection of the disease.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jianglong Kong, Rui Zou, Ga-Lai Law, Yi Wang
Summary: In this study, a NIR PL nanoparticle TRZD was synthesized, which exhibited excellent rechargeable NIR PL in vivo and could be used for long-term autofluorescence-free imaging. TRZD could inhibit the tumor growth of glioma models and provide a promising multifunctional nanoparticle for theranostics of brain diseases.
Article
Nanoscience & Nanotechnology
Daihua Fu, Fan Yang, Jiayi Zhang, Zhen Xiang, Yunbing Wang
Summary: This study reports a background interference-free persistent luminescent implant (PLI) with excellent luminescent performance, which can be detected by an optical imaging system for a long time after implantation. The implant has good biocompatibility and is not affected by tissue autofluorescence interference. This research provides new insights for designing biomedical imaging systems.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Qing Liu, Hong-Bo Cheng, Rui Ma, Mingming Yu, Yuanyu Huang, Lele Li, Jian Zhao
Summary: A near-infrared light-guided DNA nanodevice is developed for precise drug delivery and tumor therapy. The nanodevice consists of GC-rich DNA duplexes with a UV light labile moiety and upconversion nanoparticles. Upon NIR light irradiation, the nanoparticles emit UV light to break the pho-tolabile moiety, resulting in controlled drug release and fluorescence signal recovery. This modular strategy enables remotely controlled drug delivery and in situ imaging.
Article
Medicine, Research & Experimental
Ai Li, Tianyuan Zhang, Ting Huang, Ruyi Lin, Jiafu Mu, Yuanqin Su, Hao Sun, Xinchi Jiang, Honghui Wu, Donghang Xu, Hongcui Cao, Xiaoyi Sun, Daishun Ling, Jianqing Gao
Summary: In this study, gene transfection using magnetosome-like ferrimagnetic iron oxide nanochains (MFIONs) achieved high expression of HSV-tk in MSCs and promoted gap junction intercellular communication (GJIC) between MSCs and tumor cells, leading to significantly inhibited growth of glioma through an improved bystander effect. The results suggest that iron oxide nanoparticles have the potential to enhance suicide gene expression levels in MSCs and improve the sensitivity of glioma cells to HSV-tk/GCV suicide gene therapy.