Review
Biotechnology & Applied Microbiology
Wen Guo, Xindan Zhang, Jiliang Zhai, Jiajia Xue
Summary: This article introduces the potential of neural stem cells (NSCs) in repairing spinal cord injuries. NSCs, as multipotent stem cells, can differentiate into neurons and neuroglial lineages, making them an ideal choice for regenerating injured spinal cords. The article also discusses the sources and therapeutic potential of NSCs and introduces some relevant pre-clinical studies and clinical trials.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Yiyan Zheng, Chrystine M. Gallegos, Haipeng Xue, Shenglan Li, Dong H. Kim, Hongxia Zhou, Xugang Xia, Ying Liu, Qilin Cao
Summary: Recovering locomotor function after spinal cord injury (SCI) is crucial. Stem cell therapy, particularly using patient-specific induced pluripotent stem cells (iPSCs), holds promise as a reparative strategy. This study found that purified iPSC-derived neural progenitor cells (NPCs) were able to improve locomotor function in a rat model of cervical SCI.
Article
Engineering, Biomedical
Zhifeng You, Xu Gao, Xinyi Kang, Wen Yang, Tiandi Xiong, Yue Li, Feng Wei, Yan Zhuang, Ting Zhang, Yifu Sun, He Shen, Jianwu Dai
Summary: Neural regeneration after spinal cord injury (SCI) is closely related to the formation of microvascular endothelial cells (MECs) mediated neurovascular unit. This study established a method for isolating primary spinal cord-derived MECs (SCMECs) with high cell yield and purity to investigate their therapeutic effects on SCI. Transcriptomics and proteomics identified differentially expressed genes and proteins in SCMECs that were involved in angiogenesis, immunity, metabolism, and cell adhesion molecular signaling. SCMECs and brain-derived MECs (BMECs) showed different levels of angiogenesis and promoted proliferation, migration, and differentiation of spinal cord or brain-derived neural stem cells (SNSC/BNSC). SCMECs in combination with the NeuroRegen scaffold showed higher effectiveness in promoting vascular reconstruction and neuronal regeneration compared to BMECs, possibly through the VEGF/AKT/eNOS-signaling pathway.
BIOACTIVE MATERIALS
(2023)
Article
Cell Biology
Jie Chang, Zhanyang Qian, Binyu Wang, Jiang Cao, Sheng Zhang, Fan Jiang, Renyi Kong, Xiao Yu, Xiaojian Cao, Lei Yang, Hongtao Chen
Summary: This study compared the recovery after spinal cord injury (SCI) following the transplantation of A1 or A2 astrocytes. A1 astrocytes were harmful and upregulated neurotoxic genes, while A2 astrocytes were neuroprotective and upregulated the production of neurotrophic factors. Transplantation of A2 astrocytes significantly improved motor function recovery, reduced glial scar deposition, increased neurofilament formation, and showed potential therapeutic effects for SCI.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Materials Science, Biomaterials
Jun Zhou, Yaqi Wu, Zhijian Tang, Kaipeng Zou, Juan Chen, Zuowei Lei, Xueyan Wan, Yanchao Liu, Huaqiu Zhang, Yu Wang, Armin Blesch, Ting Lei, Shengwen Liu
Summary: Studies have shown that alginate capillary hydrogels can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord. Neural stem/progenitor cells (NSPCs) can survive, proliferate, and differentiate into neurons within the capillaries in vitro. In animal experiments, a portion of the grafted cells can survive and differentiate into neurons, astrocytes, and oligodendrocytes. Furthermore, the grafted cells can promote the growth of host axons, form putative synapses with host neurons, improve electrophysiological conductivity, and partially restore locomotor function.
REGENERATIVE BIOMATERIALS
(2022)
Article
Cell Biology
Shuo Liu, Yuan-Yuan Xie, Liu-Di Wang, Chen-Xu Tai, Dong Chen, Dan Mu, Yan-Yan Cui, Bin Wang
Summary: In this study, a porous collagen scaffold with axially-aligned luminal conduits was developed to enhance neural stem cell activity and cell extension, improving hindlimb motor function and nerve regeneration in a spinal cord injury model. The scaffold showed good biodegradability and elicited no notable inflammatory reaction in vivo. These findings suggest that the collagen scaffold is a promising carrier for neural stem cell transplantation to enhance spinal cord repair.
NEURAL REGENERATION RESEARCH
(2021)
Article
Neurosciences
Takeru Furumiya, Takahide Itokazu, Toru Nakanishi, Toshihide Yamashita
Summary: Schwann cells de-differentiate into repair Schwann cells after peripheral nervous system (PNS) damage and migrate into the injured spinal cord. The mechanism underlying this migration remained unknown until this study, which revealed the involvement of CXCR4 signaling. The findings showed that repair Schwann cells express CXCR4 and its ligand CXCL12 is upregulated in the injured spinal cord. Pharmacological inhibition of CXCR4 signaling decreased repair Schwann cell infiltration, while administering a CXCR4 agonist increased infiltration and improved motor function.
NEUROSCIENCE RESEARCH
(2023)
Article
Neurosciences
Jiang-Hu Huang, Yong-Neng Chen, Hang He, Chun-Hui Fu, Zhao-Yi Xu, Fei-Yue Lin
Summary: This study demonstrates that Schwann cells-derived exosomes (SCs-Exos) can promote proliferation, migration, and tube formation of brain-derived endothelial cells (bEnd.3 cells). It is found that SCs-Exos express high levels of the pro-angiogenesis molecules, Integrin-beta 1. The angiogenic effect of SCs-Exos on bEnd.3 cells is dependent on Integrin-beta 1 expression. In an spinal cord injury (SCI) model, treatment with SCs-Exos attenuates tissue damage and enhances functional recovery by promoting angiogenesis, which requires the involvement of Integrin-beta 1.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Kaiyuan Yang, Jia Yang, Weitao Man, Zhe Meng, Chun-Yi Yang, Zheng Cao, Jun Liu, Kunkoo Kim, Yaosai Liu, Shuhui Yang, Yi Guo, Zhijun He, Chao Ma, Guihuai Wang, Xiumei Wang
Summary: This study developed an aligned fibrin nanofiber hydrogel modified with N-Cadherin-Fc for delivering neural stem cells (NSCs) to the injury site of spinal cord injury (SCI). The hydrogel provided multimodal cues to guide NSC functions and nerve regeneration. The hydrogel carrying exogenous NSCs significantly promoted NSC retention, immunomodulation, neuronal differentiation, and in vivo integration with inherent neurons in a rat SCI model, resulting in neural relay formation and locomotor functional recovery.
ADVANCED FIBER MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Bo Xu, Zezhu Zhou, Jiaqi Fang, Jianguang Wang, Kun Tao, Junjian Liu, Shuhao Liu
Summary: This study investigated the efficiency of exosomes produced from Schwann cells (SCDEs) in protecting against mitochondrial dysfunction and alleviating the deleterious consequences of spinal cord injury (SCI). The results showed that SCDEs effectively mitigated oxidative stress, inflammation, and necroptosis, while enhancing mitophagy. Mechanistically, SCDEs facilitated cellular mitophagy through activation of the AMPK signaling pathway.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Review
Cell Biology
Camila Marques de Freria, Erna Van Niekerk, Armin Blesch, Paul Lu
Summary: Spinal cord injury leads to irreversible functional impairment due to neuronal loss and disruption of connections, but neural stem cell therapy shows promising potential in promoting axonal regeneration and forming new connections. This therapy has implications for improving motor systems, including the corticospinal tract, and restoring sensory feedback in SCI patients.
Review
Biotechnology & Applied Microbiology
Tian-Yang Yuan, Jun Zhang, Tong Yu, Jiu-Ping Wu, Qin-Yi Liu
Summary: Spinal cord injury is a challenging central nervous system injury, and 3D bioprinting technology shows potential in nervous system repair. This review discusses the progress of spinal cord tissue engineering and the applications of 3D bioprinting in spinal cord repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zhenni Chen, Haimin Zhang, Caixia Fan, Yan Zhuang, Wen Yang, Yanyan Chen, He Shen, Zhifeng Xiao, Yannan Zhao, Xiaoran Li, Jianwu Dai
Summary: Aligned collagen-fibrin hydrogels with stretchable property, adhesive behavior, and controlled delivery capability were developed for spinal cord injury repair. The hydrogels demonstrated improved mechanical properties compared to collagen-only hydrogels and promoted endogenous neural stem/progenitor cell migration and differentiation, leading to effective recovery.
Article
Engineering, Biomedical
Xiaoyun Liu, Mingming Hao, Zhongjin Chen, Ting Zhang, Jie Huang, Jianwu Dai, Zhijun Zhang
Summary: 3D bioprinting has been used to fabricate NSC-laden scaffolds for in vivo SCI repair, utilizing a novel bioink with fast gelation and high NSC viability. This method promotes axon regeneration and reduces glial scar deposition, leading to significant locomotor recovery in SCI model rats. It represents a versatile strategy for precise engineering of the central nervous system and other neural tissues for regenerative medicine applications.
Article
Chemistry, Multidisciplinary
Jessica Aijia Liu, Kin Wai Tam, Yong Long Chen, Xianglan Feng, Christy Wing Lam Chan, Amos Lok Hang Lo, Kenneth Lap-Kei Wu, Man-Ning Hui, Ming-Hoi Wu, Ken Kwok-Keung Chan, May Pui Lai Cheung, Chi Wai Cheung, Daisy Kwok-Yan Shum, Ying-Shing Chan, Martin Cheung
Summary: The study found that neural stem cells derived from human pluripotent stem cells have therapeutic potential for spinal cord injury. By reducing the expression of the SOX9 gene, the neurogenic capacity of the transplanted cells is enhanced, leading to improved integration and functional recovery.
Article
Medicine, Research & Experimental
V. V. Yuzhakov, I. V. Malinova, M. V. Kiseleva, N. K. Fomina, L. N. Bandurko, E. V. Komarova, L. E. Sevan'kaeva, I. E. Ingel', N. D. Yakovleva, A. D. Kaprin
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2018)
Article
Medicine, Research & Experimental
A. F. Tsyb, V. V. Yuzhakov, L. M. Roshal, G. T. Sukhikh, A. G. Konoplyannikov, G. N. Sushkevich, N. D. Yakovleva, I. E. Ingel, L. N. Bandurko, L. E. Sevan'kaeva, L. N. Mikhina, N. K. Fomina, M. V. Marei, Zh. B. Semenova, O. A. Konoplyannikova, S. Sh. Kal'sina, L. A. Lepekhina, I. V. Semenkova, E. V. Agaeva, A. S. Shevchuk, L. N. Pavlova, O. Yu. Tokarev, O. V. Karaseva, T. A. Chernyshova
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2009)
Article
Genetics & Heredity
Ivan B. Filippenkov, Vasily V. Stavchansky, Alina E. Denisova, Vadim V. Yuzhakov, Larisa E. Sevan'kaeva, Olga Y. Sudarkina, Veronika G. Dmitrieva, Leonid Gubsky, Nikolai F. Myasoedov, Svetlana A. Limborska, Lyudmila Dergunova
Article
Medicine, Research & Experimental
O. B. Abramova, V. V. Yuzhakov, M. A. Kaplan, V. V. Drozhzhina, E. A. Beregovskaya, T. P. Churikova, L. E. Sevan'kaeva, N. K. Fomina, M. G. Tsyganova, S. A. Ivanov, A. D. Kaprin
Summary: The study demonstrated that Photoran E6 was highly effective in photodynamic therapy for sarcoma M-1, with 100% of cured animals and no live tumor cells expressing mutant p53 protein in areas of photodynamic exposure.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Olga Yu Sudarkina, Ivan B. Filippenkov, Vasily V. Stavchansky, Alina E. Denisova, Vadim V. Yuzhakov, Larisa E. Sevan'Kaeva, Liya V. Valieva, Julia A. Remizova, Veronika G. Dmitrieva, Leonid Gubsky, Nikolai F. Myasoedov, Svetlana A. Limborska, Lyudmila Dergunova
Summary: Research suggests that Semax might exert neuroprotective effects by suppressing inflammation and cell death processes while activating recovery processes at both the transcriptome and protein levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marina Filimonova, Alina Saburova, Victoria Makarchuk, Ljudmila Shevchenko, Valentina Surinova, Vadim Yuzhakov, Nina Yakovleva, Larisa Sevankaeva, Vyacheslav Saburov, Sergey Koryakin, Petr Shegay, Andrey Kaprin, Sergey Ivanov, Alexander Filimonov
Summary: The study demonstrated that the NOS inhibitor compound T1023 can prevent complications of tumor radiation therapy by protecting the skin from acute radiation reactions, while not affecting the anti-tumor effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Meeting Abstract
Obstetrics & Gynecology
I. V. Malinova, V. V. Yuzhakov, M. V. Kiseleva, E. V. Komarova, L. N. Bandurko, L. E. Sevankaeva, A. D. Kaprin
HUMAN REPRODUCTION
(2017)
Article
Medicine, Research & Experimental
VV Popuchiev, LN Mikhina, ND Yakovleva, AG Konoplyannikov, VV Yuzhakov, EA Odintsova, RA Brodskii, LE Sevan'kaeva, K Kita, I Kita
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2003)