Journal
ANALYTICAL SCIENCES
Volume 34, Issue 9, Pages 1055-1059Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.18SCN01
Keywords
Imaging mass spectrometry; Drosophila melanogaster; GABA; on-tissue derivatization
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Drosophila melanogaster is a model organism in neurodegenerative disease research. In neurodegenerative study, the direct spatial information of neurotransmitters such as gamma-aminobutyric acid (GABA) in the brain of Drosophila melanogaster is important to understand the role of GABA. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is an attractive method for direct visualization of neurotransmitters. In this paper, we describe methods to visualize GABA in the brain and head of Drosophila melanogaster using MALDI-IMS.
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