Journal
ANALYTICAL SCIENCES
Volume 25, Issue 8, Pages 1013-1017Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.25.1013
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Funding
- National Nature Science Foundation of China [20635020]
- State Key Laboratory of Electroanaytical Chemistry [2006004]
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A uric acid biosensor based on the direct electron transfer of a hemoglobin-encapsulated chitosan-modified glassy carbon electrode was developed for a highly sensitive and selective analysis in urine samples. The modified electrode was prepared by the encapsulation of hemoglobin and unease in a chitosan matrix. The hydrogen peroxide produced from the catalytic oxidation of uric acid by uricase was reduced electrocatalytically by immobilized hemoglobin and used to obtain a sensitive amperometric response to uric acid. The linear response of the uric acid concentrations ranged from 2.00 to 30.0 mu M with a correlation of 0.9982, the detection limit of uric acid was estimated to be 0.85 mu M at a signal/noise ratio of 3. The uric acid biosensor can efficiently exclude the interference of commonly coexisted ascorbic acid, dopamine, epinephrine, etc. The relative standard deviation was under 2.56% (n = 5) for the determination of real samples. This biosensor is satisfactory for the determination of human urine samples compared with the HPLC-UV method.
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