Journal
ANALYTICAL SCIENCES
Volume 25, Issue 2, Pages 149-151Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.25.149
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Funding
- Microgenics Corporation
- Institute of Microchemical Technology, Co., Ltd., (IMT)
- Science and Technology
- JSPS [17310087]
- Grants-in-Aid for Scientific Research [17310087] Funding Source: KAKEN
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We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme beta-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 mu g mL(-1). Our CEDIA using a microfluidic device was very simple and rapid, unlike microchip-based heterogeneous immunoassays and CEDIA on a well-type microchip.
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