Article
Chemistry, Organic
Artur Kasprzak, Aleksandra Gajda-Walczak, Agata Kowalczyk, Barbara Wagner, Anna M. Nowicka, Mikey Nishimoto, Mariola Koszytkowska-Stawinska, Hidehiro Sakurai
Summary: This paper describes the synthesis and characterization of novel monoferrocenylsumanenes using the Sonogashira cross-coupling or click chemistry reaction and their application in cesium cation electrochemical sensors. Efficient synthetic methods for modifying sumanene at the aromatic position were developed, and the synthesized sumanene derivatives were characterized using spectroscopic analyses. The supramolecular interactions between cesium cations and the synthesized monoferrocenylsumanenes were investigated spectroscopically and electrochemically, and these derivatives were found to be highly selective and sensitive for cesium cation detection.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Sedat Karabulut, Dananjana V. Wijerathne, James W. Gauld
Summary: Cyclic peptides play important roles in biology and have potential applications in the pharmaceutical and other industries. Although thiols and amines can react to form S-N bonds, only a few cyclic peptides containing such bonds have been identified in biological systems. Density functional theory-based calculations were used to study the formation and structure of S-N containing cyclic peptides from linear peptides. The nature of the vicinal residue of cysteine can affect ring formation through stabilizing or destabilizing intramolecular interactions.
Article
Biophysics
Philip Gillespie, Robert B. Channon, Xiaotong Meng, Md Nazmul Islam, Sylvain Ladame, Danny O'Hare
Summary: Short single-stranded nucleic acids found in bodily fluids, particularly miR-141, have emerged as minimally invasive biomarkers for diseases such as cancer. This study introduces a novel sensing strategy using electrochemically active molecular probes based on PNA scaffolds for detecting short nucleic acids, demonstrating promising potential for fast, low-cost, quantitative, and sequence-specific detection of nucleic acids from liquid biopsies through EOTRs.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Shuichi Takeda, Ryotaro Koike, Ikuko Fujiwara, Akihiro Narita, Makoto Miyata, Motonori Ota, Yuichiro Maeda
Summary: Twinfilin interacts with actin capping protein (CP) in a unique way, regulating CP structure distinct from CARMIL. TWtail and CARMIL CPI restrict CP in different conformations, indicating their unique binding modes with CP.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Lucia G. Sousa, Joana Castro, Angela Franca, Carina Almeida, Christina A. Muzny, Nuno Cerca
Summary: Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age, especially posing serious health complications in pregnant women. Research has identified Fannyhessea vaginae and Gardnerella spp. as key microorganisms associated with BV, and a new PNA probe shows promising sensitivity and specificity for the detection of BV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Spectroscopy
Mei Zhao, Yu-Shuang Guo, Guo-Dong Fu, An-Qi Xue, Qing-Hao Shao, Qiong Wang, Dian-Shun Guo
Summary: The novel near-infrared optical and redox-active receptor can detect Hg2+ in water at ppb level with low detection limit and multi-feature analysis functions, showing high sensitivity to Hg2+. A possible sensing model was established through theoretical studies.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Organic
Anna-Lena Johanna Segler, Snorri Th Sigurdsson
Summary: The synthesis and characterization of rigid spin labels for DNA and RNA were reported, with single-crystal X-ray crystallography and continuous-wave EPR spectra analysis. The results showed that these labels are nonperturbing of duplex structure.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Review
Chemistry, Analytical
Pandiyaraj Kanagavalli, Chrysanthus Andrew, Murugan Veerapandian, Mani Jayakumar
Summary: This review provides a comprehensive overview of scalable fabrication methods and applications of hybridized graphene nanostructures as label-free electrochemical biosensor platforms, with a focus on the use of electrodeposition and electroless deposition techniques, as well as surface functionalization strategies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biology
Nadine T. T. Werner, Philipp Hoegel, Gokhan Guener, Walter Stelzer, Manfred Wozny, Marlene Assfalg, Stefan F. Lichtenthaler, Harald Steiner, Dieter Langosch
Summary: Intramembrane proteases are crucial in biology and medicine, but how they determine the cleavability of a substrate transmembrane (TM) domain remains unclear. This study shows that the conformational flexibility of the TM domain plays a role in substrate cleavability by gamma-secretase. Specifically, the N-terminal half of the TM helix and the C-terminal TM sequence are important for robust cleavability. The results further enhance our understanding of the mechanisms underlying intramembrane proteolysis.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kyle S. Gregory, Anna R. Newell, Otsile O. Mojanaga, Sai Man Liu, K. Ravi Acharya
Summary: This study determined the crystal structure of botulinum neurotoxin A6 cell binding domain (H-C/A6) in complex with GD1a and presented a new crystal form of wild type H-C/A6 (crystal form II), revealing the degree of conformational flexibility exhibited by H-C/A6.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Phuritat Kaewarsa, Tirayut Vilaivan, Wanida Laiwattanapaisal
Summary: A new PCA3 assay on an origami paper-based device was successfully developed in this study, allowing for molecular reactions and liquid control on the paper surface for quantitative detection of PCA3. The method was demonstrated to be effective for measuring PCA3 in urine matrix and human cancer cell lines, showing great potential for facile, rapid, and low-cost detection of PCA3 in real samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Organic
Raymond G. Emehiser, Karishma Dhuri, Caroline Shepard, Saswata Karmakar, Raman Bahal, Patrick J. Hrdlicka
Summary: Three probe chemistries were evaluated for their performance in DNA targeting experiments. Among them, Invader probes showed the most favorable and consistent recognition characteristics, while S gamma PNA probes exhibited higher recognition efficiency but lower specificity.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Chemistry, Analytical
Haixia Wang, Xuejiao Wang, Mingyuan Zhao, Chao Shi, Cuiping Ma
Summary: A novel biosensor for nucleic acid detection based on ratiometric signal detection using the oxidation peak current ratio between ferrocene and methylene blue has been developed, showing high stability, good selectivity, and improved specificity. This biosensor demonstrated a linear relationship between the ratio of I-Fc/I-MB and DNA concentration, with a high sensitivity and low detection limit. Additionally, the biosensor exhibited good selectivity by identifying single-base mismatch targets, indicating great potential for applications in nucleic acid detection and medical clinical diagnosis.
ANALYTICAL LETTERS
(2021)
Article
Biophysics
Agustin Robles-Remacho, M. Angelica Luque-Gonzalez, F. Javier Lopez-Delgado, Juan J. Guardia-Monteagudo, Mario Antonio Fara, Salvatore Pernagallo, Rosario M. Sanchez-Martin, Juan Jose Diaz-Mochon
Summary: The detection of genomic variations in repetitive sequences is crucial for understanding their biological implications. However, there is a lack of techniques to validate the sequencing data obtained from repetitive sequences, especially in the case of single-nucleotide variations (SNVs). This study presents chemFISH, an innovative method for detecting SNVs in repetitive sequences, using dynamic chemistry labelling and abasic peptide nucleic acid probes. ChemFISH allows for rapid and accurate detection of alpha-satellite DNA with single-base resolution. The method demonstrates high sensitivity and specificity, making it a potential tool for validating and discovering SNVs in repetitive sequences.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Mingyuan Zhao, Xiaoli Zhao, Ritong Sun, Jian Zhang, Haixia Wang, Chao Shi, Cuiping Ma, Yong Li
Summary: A simple electrochemical DNA biosensor was developed for rapid and specific determination of DNA using electrical potential-assisted hybridization. The biosensor showed rapid detection capability with controlled potential, linear relationship with target DNA concentrations, and good specificity for distinguishing mismatched sequences. It is a promising diagnostic tool for DNA detection.
ANALYTICAL LETTERS
(2022)
Article
Multidisciplinary Sciences
Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Hiroaki Sato, Masaki Torimura
Article
Chemistry, Analytical
Kaori Shigeta, Hiroaki Tao, Katsuhiro Nakagawa, Tomoaki Kondo, Tetsuya Nakazato
ANALYTICAL SCIENCES
(2018)
Article
Biochemistry & Molecular Biology
Hidenori Tani, Taro Matsutani, Hiroshi Aoki, Kaoru Nakamura, Yu Hamaguchi, Tetsuya Nakazato, Michiaki Hamada
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biophysics
Hiroshi Aoki, Masaki Torimura, Tetsuya Nakazato
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Biophysics
Hiroshi Aoki, Robert M. Corn, Brandon Matthews
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Instruments & Instrumentation
Hiroshi Aoki, Takeshi Sukegawa, Masaki Torimura, Tetsuya Nakazato
SENSORS AND MATERIALS
(2020)
Article
Instruments & Instrumentation
Hiroshi Aoki, Tetsuya Nakazato
SENSORS AND MATERIALS
(2020)
Article
Instruments & Instrumentation
Yasumasa Kanekiyo, You Mitani, Miho Suda, Hiroshi Aoki, Hironobu Minai
SENSORS AND MATERIALS
(2020)
Article
Pharmacology & Pharmacy
Hiroshi Aoki, Hidenori Tani, Kaoru Nakamura, Hiroaki Sato, Masaki Torimura, Tetsuya Nakazato
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2020)
Correction
Chemistry, Physical
Haoyi Wu, Xiao-Li Wu, Zheng-Ming Wang, Hiroshi Aoki, Shuzo Kutsuna, Keiko Jimura, Shigenobu Hayashi
APPLIED CATALYSIS B-ENVIRONMENTAL
(2020)
Article
Chemistry, Analytical
Hiroshi Aoki, Masaki Torimura, Hiroshi Habe
Summary: This study explores a method to discriminate microplastics by analyzing the change in fluorescent properties after adsorption of fluorescent dyes onto microplastic surfaces. Fluorescein and Methylene Blue showed increased fluorescence intensity, while Rhodamine 6G showed negligible changes. This research demonstrates the potential to identify and estimate individual plastic materials by investigating appropriate fluorescent dyes.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Hiroshi Aoki
Summary: A novel technique for microscopic observation of single microplastic particles stained with fluorescent dyes has been developed, enabling fluorescence-based determination of the materials of these particles as a first screening of material determination. Commercially available microplastic particles and fluorescent dyes were used to observe the degree of fluorescent intensity for various combinations, revealing statistically significant differences in fluorescent intensity for different materials.
Article
Chemistry, Multidisciplinary
Xinping Liu, Zhengqing Yan, Yuhuan Sun, Jinsong Ren, Xiaogang Qu
CHEMICAL COMMUNICATIONS
(2017)
Article
Chemistry, Physical
Haoyi Wu, Xiao-Li Wu, Zheng-Ming Wang, Hiroshi Aoki, Shuzo Kutsuna, Keiko Jimura, Shigenobu Hayashi
APPLIED CATALYSIS B-ENVIRONMENTAL
(2017)
Article
Engineering, Biomedical
Hirokazu Nosato, Hidenori Sakanashi, Eiichi Takahashi, Masahiro Murakawa, Hiroshi Aoki, Ken Takeuchi, Yasuo Suzuki
INTERNATIONAL JOURNAL OF BIOMEDICAL IMAGING
(2017)