Article
Biophysics
Nicoleta Elena Dina, Muhammad Ali Tahir, Sadia Z. Bajwa, Imran Amin, Ventsislav K. Valev, Liwu Zhang
Summary: Emerging antibiotic resistant bacteria pose a significant threat to public health, and surface-enhanced Raman scattering (SERS) shows great potential for detecting these bacteria and conducting antibiotic susceptibility testing. However, there are challenges of reliability, cost, and complexity that need to be addressed in order for SERS to be integrated into efficient point-of-care devices.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Steven Quarin, Pietro Strobbia
Summary: Accurate diagnosis is crucial in many pathologies, prompting efforts to move diagnostic methods from specialized labs to point-of-care settings. Surface-enhanced Raman scattering (SERS) sensing offers advantages like simultaneous detection of multiple biotargets and increased accuracy, making it a promising method for point-of-care testing applications. Many groups are working towards translating SERS sensing methods from lab to the point of need.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jiayu Zeng, Meiyu Dong, Bixue Zhu, Dongmei Chen, Yang Li
Summary: A new method utilizing SERS to explore adjacent base stacking in DNA was developed, allowing for accurate identification of point mutation sites and analysis of DNA frameshift mutations. The method has the potential application value in gene diagnosis field.
Article
Chemistry, Analytical
Aristea Anna Leventi, Kharmen Billimoria, Dorota Bartczak, Stacey Laing, Heidi Goenaga-Infante, Karen Faulds, Duncan Graham
Summary: Researchers developed a new quantitation model to calibrate the SERS response against the absolute concentration of SERS nanotags using spICP-MS, and demonstrated a homogeneous distribution and linear response of the nanotags in gelatin standards using laser ablation inductively coupled plasma time-of-flight mass spectrometry imaging. This novel calibration model shows the potential for quantitative SERS imaging, allowing correlation of nanoparticle concentration to biological responses for better understanding of disease models.
ANALYTICAL CHEMISTRY
(2023)
Review
Medicine, Research & Experimental
Trey W. Pittman, Daniel Balazs Decsi, Chamindie Punyadeera, Charles S. Henry
Summary: There has been a growing interest in using microfluidic devices for saliva analysis in recent years. This approach has the potential to provide more sensitive and selective analysis by collecting large quantities of saliva samples. The aim of this review is to provide an update on the use of microfluidic devices for salivary biomarker analysis.
Article
Nanoscience & Nanotechnology
Jayakumar Perumal, Hann Qian Lim, Amalina Binte Ebrahim Attia, Riazul Raziq, David Ian Leavesley, Zee Upton, U. S. Dinish, Malini Olivo
Summary: A biofunctionalized, cost-effective SERS substrate was developed for wound marker sensing, allowing for monitoring and detection of changes in proteins such as MMP-9 and TNF-alpha during wound healing. The platform demonstrated high sensitivity and selectivity, providing potential for a non-invasive, real-time measure of cutaneous wound healing.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Chemistry, Applied
Zhijian Wang, Lixin Zhang, Yisheng Chen
Summary: In this study, a HPTLC-platformed SERS detection method was developed for the screening of thiram in juice. The sample liquid was separated on HPTLC plates and the specific zone for the analyte was obtained. By infiltrating with atomized water, the band of interest was easily scraped off and eluted. A flexible and SERS-active substrate was fabricated by in-situ synthesis of gold nanoparticles within cotton fabrics. Under optimized conditions, the analyte's fingerprint-like signal at 1376 cm-1 was easily recorded by a hand-held Raman spectrometer, with LOD (0.5 mg/L), LOQ (0.9 mg/L), and reproducibility (<11.7%). The developed screening system was validated with pear, apple, and mango juice using spike-and-recovery rates (75.6 to 112.8%). This study demonstrates the feasibility of this method as a facile point-of-care testing system for pesticide screening.
Article
Biophysics
Bruno Gil Rosa, Oluwatomi E. Akingbade, Xiaotong Guo, Laura Gonzalez-Macia, Michael A. Crone, Loren P. Cameron, Paul Freemont, Kwang-Leong Choy, Firat Guder, Eric Yeatman, David J. Sharp, Bing Li
Summary: Accurate and cost-effective multiplexed immunosensors are important for early diagnosis and monitoring of progressive diseases. This review compares different techniques for the development of multiplexed immunosensors and discusses the barriers and potential solutions for translating this strategy into clinical practice.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Analytical
Chitra Padmakumari Kurup, Minhaz Uddin Ahmed
Summary: This review summarizes the recent advancements in nanozymes and their applications in point-of-care biosensors. Nanozymes, as enzyme-mimicking components in biosensing systems, offer improved performance and miniaturization of sensors. Various nanomaterials have been explored for the development of nanozyme-based biosensors, and different sensing strategies have been implemented using nanozymes as signal-producing components. Challenges, such as stability and specificity, need to be addressed for wider applications. The integration of nanozymes into point-of-care diagnostics promises to revolutionize healthcare by enhancing the accuracy and sensitivity of diagnostic tools.
Review
Chemistry, Analytical
Leyla Karadurmus, S. Irem Kaya, Ahmet Cetinkaya, Sibel A. Ozkan
Summary: Lack of timely detection or proper monitoring of diseases is still a leading cause of death. Developing a portable device similar to a portable laboratory for rapid disease diagnosis is crucial. Electroanalytical methods, particularly using nanomaterials like Mxenes, are cost-effective and highly sensitive for point-of-care diagnostics. This review provides an overview of recent studies on electrochemical POC diagnostics using Mxenes and offers insights into improving selectivity and sensitivity in sensor studies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lei Wu, Xuefeng Liu, Shenfei Zong, Zhuyuan Wang, Yiping Cui
Summary: Rapid analysis of neurotransmitters in body fluids is crucial for healthcare improvement. We developed a surface enhanced Raman spectroscopy (SERS) composite hydrogel device for the fast analysis of neurotransmitters in whole blood samples. The device combines a PEGDA/SA composite hydrogel for fast separation of small molecules and a plasmonic SERS substrate for sensitive detection of target molecules. With 3D printing, the hydrogel membrane and SERS substrate are integrated into a systematic device. The sensor achieves highly sensitive detection of dopamine in whole blood samples with a limit of detection down to 1 nM, and the entire detection process can be completed within 5 minutes. Due to its simplicity and rapid response, the device has great potential in point-of-care diagnosis and monitoring of neurological and cardiovascular diseases and disorders.
Review
Chemistry, Analytical
Md Alamgir Kabir, Hussein Zilouchian, Muhammad Awais Younas, Waseem Asghar
Summary: The dengue virus infects millions of people annually, and having access to testing is crucial in preventing future infections. There are various traditional and emerging methods for DENV testing, which can be effective in rural and low-income areas. Future guidelines are needed to enhance the efficiency of diagnostic tools during DENV recurrence or future outbreaks.
Review
Chemistry, Multidisciplinary
Jayakumar Perumal, Yusong Wang, Amalina Binte Ebrahim Attia, U. S. Dinish, Malini Olivo
Summary: The demand for reliable and robust methodology in bio-chemical sensing has led to the continuous advancement of sensor technologies, with SERS emerging as a promising analytical technique in biomedical and agri-food applications. Recent advancements in SERS have laid the groundwork for the development of point-of-care sensors with improved sensitivity and reproducibility, highlighting both opportunities and challenges in realizing commercially viable applications.
Article
Immunology
Ilesh Jani, Trevor F. Peter
Summary: Testing programs for SARS-CoV-2 have relied on PCR laboratory tests and rapid antigen assays, but their limitations in cost and accessibility have contributed to inequity in COVID-19 testing. Low-cost point-of-care nucleic acid tests have the potential to address these issues and improve pandemic preparedness, as well as strengthen primary healthcare services.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Biophysics
Nanjing Hao, Zeyu Wang, Pengzhan Liu, Ryan Becker, Shujie Yang, Kaichun Yang, Zhichao Pei, Peiran Zhang, Jianping Xia, Liang Shen, Lin Wang, Kathleen A. Welsh-Bohmer, Laurie Sanders, Luke P. Lee, Tony Jun Huang
Summary: Alzheimer's disease is a severe neurodegenerative disorder with early diagnosis being a challenge. Researchers have developed an integrated diagnostic system combining acoustics, microfluidics, and biosensors for accurate and rapid detection of AD biomarkers, showing promising results for early diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Maher M. Aljohani, Raja Chinnappan, Omar A. Alsager, Razan AlZabn, Ali Alhoshani, Karina Weber, Dana Cialla-May, Juergen Popp, Mohammed Zourob
Article
Biochemical Research Methods
Izabella J. Jahn, Anna Muehlig, Dana Cialla-May
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Spectroscopy
Dana Cialla-May, Jennifer Gadkari, Andreea Winterfeld, Uwe Huebner, Karina Weber, Gabriele Diekert, Torsten Schubert, Tobias Goris, Juergen Popp
Summary: The study focused on using surface enhanced Raman spectroscopy (SERS) to quickly identify the cofactors involved in the respiration process of S. multivorans. Through SERS analysis of membrane proteins from the bacterium under different cultivation conditions, it was found that S. multivorans cultivated with tetrachloroethene mainly contained Vitamin B-12, while those cultivated with other electron acceptors did not.
JOURNAL OF RAMAN SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Muhabat Adeola Raji, Yumna Aloraij, Fatimah Alhamlan, Ghadeer Suaifan, Karina Weber, Dana Cialla-May, Juergen Popp, Mohammed Zourob
Summary: This study developed a novel, low-cost and easy-to-use colorimetric flu virus biosensor assay. The assay utilizes a sandwich format with antibodies immobilized on cotton swabs for rapid detection of flu A and B viruses.
Review
Chemistry, Analytical
Sandeep Surendra Panikar, Dana Cialla-May, Elder De la Rosa, Pedro Salas, Juergen Popp
Summary: Efforts have been made to develop stealth SERS substrates to maintain sensing capabilities in clinical samples by reducing background noise and enhancing sensing capability. Two important methods proposed are the use of zwitterionic molecules and nanoshearing force to achieve antifouling features for point-of-care-testing (POCT). Recent developments in SERS show reliable stealth and sensitivity for diagnosis or personalized treatment in complex clinical samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Muhabat Adeola Raji, Ghadeer Suaifan, Atef Shibl, Karina Weber, Dana Cialla-May, Juergen Popp, Khaled Al-Kattan, Mohammed Zourob
Summary: There is growing evidence that contaminated hospital environments are crucial in transmitting nosocomial pathogens like MRSA. Infection control protocols rely on early laboratory detection of pathogens, but processing of environmental samples is less standardized than clinical samples. A novel pathogen aptasensor swab was developed to detect MRSA on non-absorbable surfaces, with a visual detection limit below 100 CFU/ml and a short 5-minute turnaround time. This selective tool shows promise in quick and cost-effective pathogen detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Muhabat Adeola Raji, Raja Chinnappan, Atef Shibl, Ghadeer Suaifan, Karina Weber, Dana Cialla-May, Juergen Popp, Enas El Shorbagy, Khaled Al-Kattan, Mohammed Zourob
Summary: The timely diagnosis of MRSA is crucial for reducing morbidity/mortality, but current identification methods have shortcomings. A new optical immunosensor was developed to detect MRSA on contaminated surfaces, with high specificity and sensitivity, making it a powerful point-of-care diagnostic platform.
Review
Biochemical Research Methods
Natalia E. Markina, Dana Cialla-May, Alexey V. Markin
Summary: The use of cyclodextrins (CDs) in surface-enhanced Raman spectroscopy (SERS) is still developing with less than 100 papers published since 1993. The review reveals controversial information about the efficiency of CDs in SERS analysis, and further experimental investigations are needed to estimate their real potential in SERS-based analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Dana Cialla-May, Christoph Krafft, Petra Roesch, Tanja Deckert-Gaudig, Torsten Frosch, Izabella J. Jahn, Susanne Pahlow, Clara Stiebing, Tobias Meyer-Zedler, Thomas Bocklitz, Iwan Schie, Volker Deckert, Juergen Popp
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Maher M. Aljohani, Dana Cialla-May, Juergen Popp, Raja Chinnappan, Khaled Al-Kattan, Mohammed Zourob
Summary: Aptamers are RNA/DNA oligonucleotide molecules that specifically bind to targeted molecules. They have potential diagnostic and therapeutic applications for blood diseases and offer advantages over antibodies in terms of selection, production, modification, stability, and immunogenicity. Several aptamers have been developed and investigated in various biomedical areas. The pegaptanib sodium injection, an aptamer-based therapeutic, has been approved by the FDA for therapeutic use. Other aptamers are also in clinical trials. This review highlights the current status and promising aptamers in pre-clinical development for blood diseases.
Review
Chemistry, Analytical
Stefano Fornasaro, Dana Cialla-May, Valter Sergo, Alois Bonifacio
Summary: This article presents a comprehensive review of various novel approaches based on surface-enhanced Raman scattering (SERS) for the direct and indirect detection and quantification of antibiotic, antifungal, and antituberculosis drugs in different matrices. The challenges for successful exploitation of this technique in the development of assays for point-of-care tests are particularly emphasized.
Review
Chemistry, Analytical
A. Silge, Karina Weber, D. Cialla-May, L. Mueller-Boetticher, D. Fischer, J. Popp
Summary: The application of Raman spectroscopic methods in life science has made significant progress, driven by advances in instrumentation, miniaturization, and interdisciplinary dialogue. These advancements have brought new possibilities for optical sensing and chemical imaging, contributing to advanced pharmaceutical development and manufacturing. Raman sensing, with its non-destructive and label-free quality testing capabilities, is particularly valuable for process-related aspects in bioprocessing. Additionally, the trend towards personalized medicine has increased the demand for individually tailored medicines, manufactured in public pharmacies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chen Liu, Lisa Mueller-Botticher, Chang Liu, Jurgen Popp, Dagmar Fischer, Dana Cialla-May
Summary: A Raman-based label-free analytical method was developed to detect ciprofloxacin in different matrices. The method allows for semiquantification and can overcome interference from excipients and carrier substances. Surface-enhanced Raman spectroscopy is applied for sensitive detection, and the method can also monitor the degradation of ciprofloxacin within pharmaceutical formulations.
Article
Chemistry, Physical
Isidro Badillo-Ramirez, Jose M. Saniger, Jurgen Popp, Dana Cialla-May
Summary: Dopamine (DA) detection using SERS technique showed reproducible spectra in c-AgNP medium, while a completely different SERS profile was observed in h-AgNP solution. DA oxidation and conversion to PDA were found under different pH conditions, with in situ PDA formation triggered by laser irradiation. Long laser exposure time led to the formation of stable AgNP-PDA composites.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Chemistry, Analytical
Sahar Alhogail, Raja Chinnappan, Majeda Alrifai, Ghadeer A. R. Y. Suaifan, Floris J. Bikker, Wendy E. Kaman, Karina Weber, Dana Cialla-May, Jurgen Popp, Mohamed B. Alfageeh, K. Al-Kattan, Mohammed M. Zourob
Summary: This study demonstrates the development of a sensitive, specific, and quantitative peptide-based nanoprobe prototype assay for the detection of Legionellaceae in a simple way and in a short time. The method can detect Legionella strains as low as 60 CFU mL(-1) without significant cross-reactivity to other closely associated bacterial species.
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)
