Review
Chemistry, Analytical
Bin Xiao, Ruiming Zhao, Nan Wang, Juan Zhang, Xiaoyun Sun, Ailiang Chen
Summary: Loop-mediated isothermal amplification (LAMP) is a widely used thermostatic nucleic acid amplification technology in clinical diagnosis and food safety detection. Centrifugal microfluidic chips, developed based on traditional microfluidic chips, utilize centrifugal force for liquid flow, mixing, and reaction, eliminating the need for complex valve and pump design. The combination of LAMP and centrifugal microfluidic chips holds great potential for rapid on-site nucleic acid detection.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Rui Mao, Tianzuo Wang, Yue Zhao, Xinyao Wu, Shun Zhang, Ting Cai
Summary: The article presents a novel isothermal amplification method called closed dumbbell mediated isothermal amplification (CDA) for nucleic acids. The method offers the advantages of simple primer design, high amplification efficiency, and successful detection of virus genes and bacterial pathogens. Real-time and colorimetric CDA detection methods have also been developed and proven effective.
Review
Chemistry, Analytical
So-Hee Kim, So-Young Lee, Unji Kim, Se-Wook Oh
Summary: This review outlines several techniques for reducing false-positive LAMP results before and after amplification. Organic additives or pullulan can be used to decrease nonspecific amplification before amplification. Uracil-DNA-glycosylase and the hot-start effect with gold nanoparticles can eliminate carry-over contamination and reduce nonspecific amplification after amplification. The use of guide RNA and colorimetric change in DNAzyme can accurately confirm amplification results.
ANALYTICA CHIMICA ACTA
(2023)
Review
Materials Science, Multidisciplinary
Nazente Atceken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh, Ali K. Yetisen, Savas Tasoglu
Summary: LAMP technology is a fast, efficient, and low-cost diagnostic method for pathogen detection, which can be applied to various bacteria, viruses, and parasitic pathogens. In addition, LAMP technology can also be used for SNP genotyping and mutation detection.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Rui Mao, Xinyao Wu, Qing Miao, Ting Cai
Summary: Nucleic acid-based assays, such as Loop-mediated isothermal amplification (LAMP), have been widely used in clinical diagnostics, food safety, and environmental monitoring. A novel method called asymmetric stem-loop-mediated isothermal amplification (ASLAMP) was developed to overcome the challenges of specific primer design and non-specific amplification associated with LAMP. ASLAMP showed promising results in detecting the H1N1 gene fragment with simple primer design, short target sequence, and high amplification efficiency, offering potential for point-of-care DNA isothermal amplification.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yaoqiang Shi, Lan Kang, Rongrong Mu, Min Xu, Xiaoqiong Duan, Yujia Li, Chunhui Yang, Jia-Wei Ding, Qinghua Wang, Shilin Li
Summary: Researchers have developed a novel diagnostic test using a combination of LAMP and CRISPR/Cas12a to detect S. flexneri infection quickly and reliably, with results visible to the naked eye in 40 minutes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
Alexandra Knox, Gemma Zerna, Travis Beddoe
Summary: The strengths of alkali-activated slag (AAS) initially rise and then fall as the content of municipal solid waste incineration bottom ash (MSWI-BA) increases, with the highest value observed in AAS with 6% MSWI-BA. The flexural and compressive strengths of AAS with 6% MSWI-BA at 400 degrees C are 31.3% and 17.9% higher than those of AAS without MSWI-BA, respectively. The presence of portlandite and hydrotalcite in MSWI-BA increases the alkali concentration and accelerates the hydration reaction when the MSWI-BA content is less than 6%, promoting the formation of C-A-S-H gel with a high degree of polymerization. However, excessive MSWI-BA (> 6%) leads to a decrease in the amount of C-A-S-H and a significant increase in porosity, reducing the strength of AAS.
Article
Chemistry, Analytical
You-Ru Jhou, Chih-Hung Wang, Huey-Pin Tsai, Yan-Shen Shan, Gwo-Bin Lee
Summary: An integrated microfluidic platform utilizing real-time reverse-transcription loop-mediated isothermal amplification was developed for detection and quantification of three genes of SARS-CoV-2, providing a useful tool for COVID-19 diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Chemistry, Physical
Jianping Liang, Jie Zeng, Xiaojuan Huang, Tengteng Zhu, Yonglong Gong, Chen Dong, Xiangrong Wang, Lingzhi Zhao, Lei Xie, Kang Liang, Qiongxiang Tan, Yali Cui, Biao Kong, Wenli Hui
Summary: With the increasing global threat of diseases and infections, the development of a fast, low-cost, and easy-to-use point-of-care testing system is essential. This study proposes a rapid visual detection method that integrates loop-mediated isothermal amplification and lateral flow assay for various analytes. The method does not require DNA extraction and prevents false positive results. The whole detection process can be completed within 25 minutes, and the accuracy is measured using the polymorphisms of methylenetetrahydrofolate reductase.
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)