Site-Specific PEGylation of Therapeutic Proteins

Bridging Disulfides for Stable and Defined Antibody Drug Conjugates

(2014) George Badescu et al.   BIOCONJUGATE CHEMISTRY

Engineering Protein Farnesyltransferase for Enzymatic Protein Labeling Applications

(2014) Jonathan K. Dozier et al.   BIOCONJUGATE CHEMISTRY

Intrinsic bioconjugation for site-specific protein PEGylation at N-terminal serine

(2014) Paul M. Levine et al.   CHEMICAL COMMUNICATIONS

The Top Prescription Drugs of 2012 Globally: Biologics Dominate, But Small Molecule CNS Drugs Hold on to Top Spots

(2013) Craig W. Lindsley   ACS Chemical Neuroscience

Transglutaminase-mediated macromolecular assembly: production of conjugates for food and pharmaceutical applications

(2013) L. Mariniello et al.   AMINO ACIDS

Self-Liganded Suzuki-Miyaura Coupling for Site-Selective Protein PEGylation

(2013) Anaëlle Dumas et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Mix to Validate: A Facile, Reversible PEGylation for Fast Screening of Potential Therapeutic Proteins In Vivo

(2013) Tae Hyung Kim et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Polyethylene Glycol Modified FGF21 Engineered to Maximize Potency and Minimize Vacuole Formation

(2013) Jing Xu et al.   BIOCONJUGATE CHEMISTRY

Site-Specific PEGylation of Human Thyroid Stimulating Hormone to Prolong Duration of Action

(2013) Huawei Qiu et al.   BIOCONJUGATE CHEMISTRY

Impact of Site-Specific PEGylation on the Conformational Stability and Folding Rate of the Pin WW Domain Depends Strongly on PEG Oligomer Length

(2013) Brijesh K. Pandey et al.   BIOCONJUGATE CHEMISTRY

Facile and Stabile Linkages through Tyrosine: Bioconjugation Strategies with the Tyrosine-Click Reaction

(2013) Hitoshi Ban et al.   BIOCONJUGATE CHEMISTRY

A Highly Efficient Catalyst for Oxime Ligation and Hydrazone–Oxime Exchange Suitable for Bioconjugation

(2013) Mohammad Rashidian et al.   BIOCONJUGATE CHEMISTRY

Enzymatic Labeling of Proteins: Techniques and Approaches

(2013) Mohammad Rashidian et al.   BIOCONJUGATE CHEMISTRY

Chemical and Enzymatic Site Specific PEGylation of hGH

(2013) Débora da Silva Freitas et al.   BIOCONJUGATE CHEMISTRY

Site-specific PEGylation of a mutated-cysteine residue and its effect on tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)

(2013) Li-Qiang Pan et al.   BIOMATERIALS

Protein cell-surface display through in situ enzymatic modification of proteins with a poly(Ethylene glycol)-lipid

(2013) Urara Tomita et al.   BIOTECHNOLOGY AND BIOENGINEERING

A novel B-domain O-glycoPEGylated FVIII (N8-GP) demonstrates full efficacy and prolonged effect in hemophilic mice models

(2013) H. R. Stennicke et al.   BLOOD

Protein conjugation with genetically encoded unnatural amino acids

(2013) Chan Hyuk Kim et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Development of a C-Terminal Site-Specific PEGylated Analog of GLP-1 with Improved Anti-Diabetic Effects in Diabetic Mice

(2013) Mingming Gao et al.   DRUG DEVELOPMENT RESEARCH

Carbohydrate-Mediated Polyethylene Glycol Conjugation of TSH Improves Its Pharmacological Properties

(2013) Anna Park et al.   ENDOCRINOLOGY

Conjugation-Induced Fluorescent Labeling of Proteins and Polymers Using Dithiomaleimides

(2013) Mathew P. Robin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemistry for peptide and protein PEGylation

(2012) M.J. Roberts et al.   ADVANCED DRUG DELIVERY REVIEWS

Immobilized metal ion affinity chromatography: a review on its applications

(2012) Randy Chi Fai Cheung et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering of Aerococcus viridansl-Lactate Oxidase for Site-Specific PEGylation: Characterization and Selective Bioorthogonal Modification of a S218C Mutant

(2012) Birgit Unterweger et al.   BIOCONJUGATE CHEMISTRY

Development of Copper-Catalyzed Azide–Alkyne Cycloaddition for Increased in Vivo Efficacy of Interferon β-1b by Site-Specific PEGylation

(2012) Natalie W. Nairn et al.   BIOCONJUGATE CHEMISTRY

Site-Specific PEGylation at Histidine Tags

(2012) Yuehua Cong et al.   BIOCONJUGATE CHEMISTRY

Site-Specific PEGylation of HR2 Peptides: Effects of PEG Conjugation Position and Chain Length on HIV-1 Membrane Fusion Inhibition and Proteolytic Degradation

(2012) Maarten Danial et al.   BIOCONJUGATE CHEMISTRY

Site-Specific PEGylated Exendin-4 Modified with a High Molecular Weight Trimeric PEG Reduces Steric Hindrance and Increases Type 2 Antidiabetic Therapeutic Effects

(2012) Tae Hyung Kim et al.   BIOCONJUGATE CHEMISTRY

Glycoengineering Approach to Half-Life Extension of Recombinant Biotherapeutics

(2012) Chen Chen et al.   BIOCONJUGATE CHEMISTRY

Solid-phase synthesis of C-terminal peptide libraries for studying the specificity of enzymatic protein prenylation

(2012) Yen-Chih Wang et al.   CHEMICAL COMMUNICATIONS

Anti-TNF Monoclonal Antibodies in Inflammatory Bowel Disease: Pharmacokinetics-Based Dosing Paradigms

(2012) I Ordás et al.   CLINICAL PHARMACOLOGY & THERAPEUTICS

Chemoenzymatic Reversible Immobilization and Labeling of Proteins without Prior Purification

(2012) Mohammad Rashidian et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Population pharmacokinetic modeling for dose setting of nonacog beta pegol (N9-GP), a glycoPEGylated recombinant factor IX

(2012) P. W. COLLINS et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Genetically encoded alkenyl–pyrrolysine analogues for thiol–ene reaction mediated site-specific protein labeling

(2012) Yiming Li et al.   Chemical Science

N-PEGylation of a Reverse Turn Is Stabilizing in Multiple Sequence Contexts, unlike N-GlcNAcylation

(2011) Joshua L. Price et al.   ACS Chemical Biology

Recombinant Protein Hydrazides: Application to Site-Specific Protein PEGylation

(2011) Jennifer Thom et al.   BIOCONJUGATE CHEMISTRY

In Situ Maleimide Bridging of Disulfides and a New Approach to Protein PEGylation

(2011) Felix F. Schumacher et al.   BIOCONJUGATE CHEMISTRY

Longer action means better drug: Tuning up protein therapeutics

(2011) Anna Szlachcic et al.   BIOTECHNOLOGY ADVANCES

Prolonged half-life and preserved enzymatic properties of factor IX selectively PEGylated on native N-glycans in the activation peptide

(2011) H. Ostergaard et al.   BLOOD

Strategies for extended serum half-life of protein therapeutics

(2011) Roland E Kontermann   CURRENT OPINION IN BIOTECHNOLOGY

FGF21 Analogs of Sustained Action Enabled by Orthogonal Biosynthesis Demonstrate Enhanced Antidiabetic Pharmacology in Rodents

(2011) J. Mu et al.   DIABETES

Introduction to current and future protein therapeutics: A protein engineering perspective

(2011) Paul J. Carter   EXPERIMENTAL CELL RESEARCH

Biophysical characterisation of GlycoPEGylated recombinant human factor VIIa

(2011) Bitten Plesner et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Plasma Half-life Extension of Small Recombinant Antibodies by Fusion to Immunoglobulin-binding Domains

(2011) Meike Hutt et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A new method to increase selectivity of transglutaminase mediated PEGylation of salmon calcitonin and human growth hormone

(2011) Anna Mero et al.   JOURNAL OF CONTROLLED RELEASE

State of the art in PEGylation: The great versatility achieved after forty years of research

(2011) Gianfranco Pasut et al.   JOURNAL OF CONTROLLED RELEASE

FDA-approved poly(ethylene glycol)–protein conjugate drugs

(2011) Steevens N. S. Alconcel et al.   Polymer Chemistry

Optimized clinical performance of growth hormone with an expanded genetic code

(2011) H. Cho et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sortase-catalyzed transformations that improve the properties of cytokines

(2011) M. W. Popp et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Solid-Phase Synthesis of PEGylated Lipopeptides Using Click Chemistry

(2010) Rasmus I. Jølck et al.   BIOCONJUGATE CHEMISTRY

PEGylation of therapeutic proteins

(2010) Simona Jevševar et al.   Biotechnology Journal

Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

(2010) Mohammad Rashidian et al.   CHEMICAL COMMUNICATIONS

Selectively weakened binding of methotrexate by human dihydrofolate reductase allows rapid ex vivo selection of mammalian cells

(2010) Jordan P. Volpato et al.   JOURNAL OF MOLECULAR RECOGNITION

Tyrosine Bioconjugation through Aqueous Ene-Type Reactions: A Click-Like Reaction for Tyrosine

(2010) Hitoshi Ban et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Certolizumab Pegol

(2010) Niti Goel et al.   mAbs

Pegloticase

(2010) Naomi Schlesinger et al.   NATURE REVIEWS DRUG DISCOVERY

Removal of B cell epitopes as a practical approach for reducing the immunogenicity of foreign protein-based therapeutics☆

(2009) Satoshi Nagata et al.   ADVANCED DRUG DELIVERY REVIEWS

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

(2009) Ellen M. Sletten et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

New Strategy for the Extension of the Serum Half-Life of Antibody Fragments

(2009) Sabrina Trüssel et al.   BIOCONJUGATE CHEMISTRY

Nonnatural Amino Acids for Site-Specific Protein Conjugation

(2009) Albert J. de Graaf et al.   BIOCONJUGATE CHEMISTRY

Transglutaminase-Mediated PEGylation of Proteins: Direct Identification of the Sites of Protein Modification by Mass Spectrometry using a Novel Monodisperse PEG

(2009) Anna Mero et al.   BIOCONJUGATE CHEMISTRY

Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering

(2009) Shinya Tsukiji et al.   CHEMBIOCHEM

Synthesis, Properties, and Applications of Diazotrifluropropanoyl-Containing Photoactive Analogs of Farnesyl Diphosphate Containing Modified Linkages for Enhanced Stability

(2009) Marisa L. Hovlid et al.   Chemical Biology & Drug Design

Site-directed enzymatic PEGylation of the human granulocyte colony-stimulating factor

(2009) Carlo Maullu et al.   FEBS Journal

Peginterferon Alfa-2b or Alfa-2a with Ribavirin for Treatment of Hepatitis C Infection

(2009) John G. McHutchison et al.   NEW ENGLAND JOURNAL OF MEDICINE

Self-Assembly of Antibodies by Chemical Induction

(2008) Qing Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Impact of PEGylation on Biological Therapies

(2008) Francesco M Veronese et al.   BIODRUGS

The Pharmacology of PEGylation: Balancing PD with PK to Generate Novel Therapeutics

(2008) C.Simone Fishburn   JOURNAL OF PHARMACEUTICAL SCIENCES

Certolizumab pegol

(2008) Gil Y. Melmed et al.   NATURE REVIEWS DRUG DISCOVERY

Generation and biochemical characterization of glycoPEGylated factor VIIa derivatives

(2008) Henrik Østergaard et al.   THROMBOSIS AND HAEMOSTASIS

Site-specific modification and PEGylation of pharmaceutical proteins mediated by transglutaminase

(2007) Angelo Fontana et al.   ADVANCED DRUG DELIVERY REVIEWS

PEG-uricase in the management of treatment-resistant gout and hyperuricemia

(2007) Merry R. Sherman et al.   ADVANCED DRUG DELIVERY REVIEWS

Formulation of Neulasta® (pegfilgrastim)

(2007) Deirdre Murphy Piedmonte et al.   ADVANCED DRUG DELIVERY REVIEWS

Protein therapeutics: a summary and pharmacological classification

(2007) Benjamin Leader et al.   NATURE REVIEWS DRUG DISCOVERY