Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 16, Issue 3, Pages 6513-6531Publisher
MDPI AG
DOI: 10.3390/ijms16036513
Keywords
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Funding
- Japan Science and Technology Agency (JST)
- Life Science Research Fund of Takeda Science Foundation
- Japan Society for the Promotion of Science (JSPS) [24-8188, 26-9576, 25-03730]
- Core Research for Evolutional Science and Technology (CREST) of Molecular Technology and Platform for Drug Discovery, Informatics, and Structural Life Science
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- Grants-in-Aid for Scientific Research [12J08188, 14J09576] Funding Source: KAKEN
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Genetic code expansion and reprogramming methodologies allow us to incorporate non-canonical amino acids (ncAAs) bearing various functional groups, such as fluorescent groups, bioorthogonal functional groups, and post-translational modifications, into a desired position or multiple positions in polypeptides both in vitro and in vivo. In order to efficiently incorporate a wide range of ncAAs, several methodologies have been developed, such as orthogonal aminoacyl-tRNA-synthetase (AARS)-tRNA pairs, aminoacylation ribozymes, frame-shift suppression of quadruplet codons, and engineered ribosomes. More recently, it has been reported that an engineered translation system specifically utilizes an artificially built genetic code and functions orthogonally to naturally occurring counterpart. In this review we summarize recent advances in the field of ribosomal polypeptide synthesis containing ncAAs.
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