Article
Chemistry, Analytical
Xianli Chen, Yong Liang
Summary: A differential colorimetric sensor array was developed for the simultaneous and accurate identification of nine proteins, showing promising potential for early disease diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Eider Berganza, Mirsana P. Ebrahimkutty, Srivatsan K. Vasantham, Chunting Zhong, Alexander Wunsch, Alexander Navarrete, Milos Galic, Michael Hirtz
Summary: The study introduces a simple and low-cost platform for curvature sensitive protein screening, enabling control over lipid composition and membrane curvature, thus providing a new approach for screening curvature-dependent protein-membrane interaction studies.
Article
Energy & Fuels
Merwan Benhabib, Samuel L. Kleinman, Mark C. Peterman
Summary: Amines are used in various processes in the oil and gas industry, but their presence can cause problems such as corrosion and nitrification. Traditional methods of amine analysis are not suitable for field deployment. We propose a new method using surface-enhanced Raman spectroscopy for accurate and quick quantification of problem-causing amines. This method is stable, calibration-free, and can be performed by minimally trained operators within 5 minutes.
Article
Energy & Fuels
Merwan Benhabib, Samuel L. Kleinman, Mark C. Peterman
Summary: Amines are utilized in various areas of the petroleum industry for different purposes such as hydrogen sulfide removal, corrosion prevention, and pH control. However, leftover amines can negatively affect the refining process by causing corrosion or nitrification. Traditional analytical methods like ion chromatography are inconvenient for field use. This study presents a new calibration-free technique for amine quantification using surface-enhanced Raman spectroscopy, specifically targeting problem-causing amines like monoethanolamine and methylamine. This method is accurate, stable, and can be performed quickly by minimally trained operators.
Article
Engineering, Environmental
Haichao Li, Zejun Deng, Zengkai Jiao, Ruitong Zhu, Li Ma, Kechao Zhou, Zhiming Yu, Qiuping Wei
Summary: In this study, a fouling-resistant nanoporous diamond sensing interface called NanoDiaSens was engineered. It allows reliable voltammetric quantification of the target substance dopamine in human serum. NanoDiaSens possesses stable electroactive gold nanoparticles and the capability to eliminate the response of interfering substances, exhibiting excellent electrocatalytic properties.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Elberth M. Schiefer, Andressa F. Santos, Marcia Muller, Andrea E. M. Stinghen, Lucas H. Negri, Jose L. Fabris
Summary: This study presents a colorimetric sensor based on albumin bound to citrate-capped silver nanoparticles for detecting protein-bound uremic toxins, such as indoxyl sulfate and p-cresylsulfate. The sensor method exhibits a linear response and allows for distinguishing between normal and uremic blood concentrations of toxins, reducing analysis costs. The interaction between albumin and citrate-capped silver nanoparticles was also investigated through Raman spectroscopy.
IEEE SENSORS JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Antonio Minopoli, Emanuela Scardapane, Bartolomeo Della Ventura, Julian A. Tanner, Andreas Offenhaeusser, Dirk Mayer, Raffaele Velotta
Summary: A novel double-resonant plasmonic substrate for fluorescence amplification in a chip-based apta-immunoassay was reported. The substrate relies on the plasmon-enhanced fluorescence (PEF) effect to amplify the fluorescence signal. It can potentially be used for multiplexing applications. In a malaria apta-immunoassay, the substrate successfully detected Plasmodium falciparum lactate dehydrogenase (PfLDH) in human whole blood with high sensitivity and without complex pretreatments.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Kenta Hagiya, Akihisa Miyagawa, Shigenori Nagatomo, Kiyoharu Nakatani
Summary: In this study, protein detection at zmol or pM levels on carboxy-functionalized polystyrene microparticles using zeta change was successfully demonstrated. The pH dependence of zeta for the protein-modified particles was well-explained, and an increase in the binding number of proteins per single PS particle led to a decrease in zeta, consistent with the proposed model. The detection limits for different proteins were determined.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Maria Andrea Molina Torres, Alicia Viviana Veglia, Natalia Lorena Pacioni
Summary: The method developed in this study utilizes the fluorescence quenching of rhodamine-6G to quantify gold and silver nanoparticles in aqueous systems. Validation through recovery tests in spiked drinking and surface river water demonstrated the accuracy and reliability of the method, making it a rapid and cost-effective approach for analyzing metal nanoparticles.
MICROCHEMICAL JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Asta Kausaite-Minkstimiene, Anton Popov, Almira Ramanaviciene
Summary: A surface plasmon resonance (SPR) immunosensor signal amplification strategy based on antibody-functionalized gold-coated magnetic nanoparticles (mAuNPs) was developed for ultrasensitive and quantitative detection of the CD5 biomarker. The sandwich immunoassay utilizing mAuNPs-anti-CD5(2B) significantly improved the detection sensitivity and showed satisfactory performance in human blood serum.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemical Research Methods
David Dierks, Miguel Angel Garcia-Campos, Anna Uzonyi, Modi Safra, Sarit Edelheit, Alice Rossi, Theodora Sideri, Radhika A. Varier, Alexander Brandis, Yonatan Stelzer, Folkert van Werven, Ruth Scherz-Shouval, Schraga Schwartz
Summary: m6A-seq2 is a method that uses multiplexed N-6-methyladenosine immunoprecipitation to compare m6A levels at the site, gene, and sample level, increasing throughput and reducing technical variability and cost. It provides sample-level relative quantitation of m6A and gene-level quantitation, showing that m6A is a main driver of RNA stability in mouse embryonic stem cells.
Article
Chemistry, Analytical
Qi Kang, Beibei Chen, Man He, Bin Hu
Summary: A simple and direct ICP-MS-based assay was developed for the simultaneous detection of three miRNAs by combining the lanthanide labeling strategy with entropy-driven catalytic amplification. This method allows for flexible probe design, good sensitivity, and excellent specificity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qinglei Shu, Yafei Zhu, Yipi Xiao, Kaisen Chen, Xi Mai, Xiangjuan Zheng, Xiluan Yan
Summary: The establishment of stable and simple detection methods for cancer markers is crucial for cancer treatment. In this study, dual aptamers for carcinoembryonic antigen (CEA) were used to specifically recognize CEA and form a sandwich structure. Two different strategies, one using magnetic beads and nickel hexacyanoferrate nanoparticles (Ni NPs) and the other using streptavidin-modified gold nanoparticles (Au NPs), were employed to achieve signal cascade amplification. The detection limits of the two methods were very low, allowing for the detection of protein biomarkers at extremely low concentrations in a small amount of serum, which has great significance for the early diagnosis of cancer.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Lu Shi, Qiaorong Tang, Bing Yang, Wei Liu, Baoxin Li, Chaoyong Yang, Yan Jin
Summary: A dual-mode DNA logic gate based on gas pressure biosensing and lateral flow assay was developed for the intelligent detection of multiple microRNAs. The gate utilized platinum-coated gold nanoparticles as a signal reporter for dual-signal readout. The miRNAs initiated the cyclic strand displacement reaction to enrich a large amount of nanoparticles, which could be visually detected on a lateral flow strip. Additionally, the nanoparticles catalyzed the decomposition of H2O2 to generate gas pressure, which could be measured by a handheld gas pressure meter.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Jia-Huan Qu, Bernd Peeters, Filip Delport, Karen Vanhoorelbeke, Jeroen Lammertyn, Dragana Spasic
Summary: This study introduces an innovative multiplexing concept on a fiber optic surface plasmon resonance platform, demonstrating the simultaneous detection of two targets using the same sensor probe. By utilizing Co (III)-NTA chemistry and gold nanoparticles in a unique way, the study achieved discrimination of two targets in the same sample. Through optimizing the bioreceptor surface density and immobilization order, the study achieved optimal detection of both targets and established calibration curves in buffer and diluted human blood plasma. The results indicate the potential for a sensitive and versatile biosensing platform with applications in diagnostics and life sciences.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)