Article
Biotechnology & Applied Microbiology
Jinwoong Lee, Yasushi Iwata, Yuji Suzuki, Iwane Suzuki
Summary: This study investigated the phosphate uptake in cyanobacteria cells under high Pi conditions, revealing a unique stimulation of Pi uptake after chlorosis and the mechanism of Pi uptake induced by sulfate deficiency. It also identified an ABC transporter involved in rapid Pi uptake under sulfate deficiency. These findings offer a new perspective on Pi uptake under high Pi conditions in cyanobacteria and bacteria.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Plant Sciences
Wen Liu, Shahbaz Khan, Mengying Tong, Haiyan Hu, Liyan Yin, Jiaquan Huang
Summary: In this study, we identified seven MGT genes (SlMRS2) in tomato and demonstrated their high-affinity transport of Mg2+ through functional analysis in a bacterial strain. The expression of these Mg2+ transporters was down-regulated under Mg2+ limitation and up-regulated with a circadian rhythm under Mg2+ toxicity in leaves. Our findings lay the groundwork for further analysis of the distinct functions of SlMRS2 family members in tomato.
Article
Plant Sciences
Ahmad Hassan Chaudhry, Syed Bilal Hussain, Wei Du, Yongzhong Liu, Shu-Ang Peng, Xiuxin Deng, Zhiyong Pan
Summary: Interveinal chlorosis, a common problem in citrus orchards in southern China, was investigated in this study. The 'Langfeng' navel orange (LF) grafted onto a Trifoliate orange (TO) rootstock had healthy green leaves, while the 'Newhall' navel orange (NHE) grafted onto TO showed typical magnesium (Mg) deficiency-induced chlorosis. This suggests that Mg deficiency may be the primary cause of chlorosis in NHE/TO.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyu Yang, Natsuko Kobayashi, Yoshiki Hayashi, Koichi Ito, Yoshitaka Moriwaki, Tohru Terada, Kentaro Shimizu, Motoyuki Hattori, Ren Iwata, Hisashi Suzuki, Tomoko M. Nakanishi, Keitaro Tanoi
Summary: This study investigates the significance of the conserved GMN motif in the Arabidopsis thaliana AtMRS2-1 transporter for magnesium transport, using structural prediction and mutagenesis experiments.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Ming Li, Yang Li, Yue Lu, Jianhui Li, Xuhang Lu, Yue Ren, Tianlei Wen, Yaojie Wang, Shenghai Chang, Xing Zhang, Xue Yang, Yuequan Shen
Summary: This study presents the cryo-EM structures of human Mrs2, revealing its symmetrical pentameric assembly and the mechanism of Mg2+ permeation. The findings provide important insights into the channel assembly and Mg2+ permeation of Mrs2.
NATURE COMMUNICATIONS
(2023)
Article
Nutrition & Dietetics
Marlene Fabiola Escobedo-Monge, Enrique Barrado, Joaquin Parodi-Roman, Maria Antonieta Escobedo-Monge, Maria Carmen Torres-Hinojal, Jose Manuel Marugan-Miguelsanz
Summary: This study evaluated the magnesium status and nutritional indicators in 78 children and adolescents with chronic diseases. The results showed that 79% of individuals had abnormal magnesium status and were at risk of developing other chronic illnesses.
Article
Biochemistry & Molecular Biology
Qiuxiang Ma, Yancai Feng, Shu Luo, Lu Cheng, Weijing Tong, Xinlu Lu, Youzhi Li, Peng Zhang
Summary: Aquaporins, particularly MePIP2;7, play a crucial role in the synergistic transport of Mg-2(+) and water in cassava. MePIP2;7 enhances the transportability of Mg-2(+) by interacting with MeMGT9, a magnesium transporter protein, thereby regulating the absorption and transport of water and Mg(2)(+) in cassava.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Louis Tung Faat Lai, Jayashree Balaraman, Fei Zhou, Doreen Matthies
Summary: Magnesium ions (Mg2+) are crucial for cellular physiology, and MRS2 is a channel responsible for regulating Mg2+ influx and homeostasis in mitochondria. This study provides insights into the gating residues and Mg2+ binding sites of MRS2, shedding light on the translocation and regulatory mechanisms of Mg2+.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Ahmad Humayan Kabir, Sharaban Tahura, Mona M. Elseehy, Ahmed M. El-Shehawi
Summary: This study investigated the responses and molecular characterization of genes in Fe-deficient sunflower plants. The results showed that Fe deficiency led to decreased plant biomass, photosynthetic efficiency, and concentrations of Fe and Zn in roots and shoots. Furthermore, it was observed that the expression of specific genes related to Fe and Zn uptake was affected by Fe shortage in sunflower plants.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Alaumy Joshi, Vishal M. Gohil
Summary: Barth syndrome is a debilitating disorder caused by mutations in the TAFAZZIN gene, which is involved in maintaining normal levels of cardiolipin in mitochondrial membranes. A recent study found that in addition to calcium, the levels of magnesium are significantly reduced in Barth syndrome patients. This study also identified a decrease in the abundance of the mitochondrial magnesium influx channel MRS2 in various models of Barth syndrome. The researchers attributed this reduction to the increased turnover of MRS2 in models with cardiolipin deficiency. These findings suggest that perturbation of mitochondrial magnesium homeostasis may contribute to the pathology of Barth syndrome.
HUMAN MOLECULAR GENETICS
(2023)
Article
Medicine, General & Internal
Chia-Chieh Lee, Po-Kai Yang, Li-Ching Chen, Mei-Leng Cheong, Ya-Lun Tsai, Ming-Song Tsai
Summary: This study aimed to assess the relationship between gene expressions of magnesium transporters and glucose parameters in pregnant women. It was found that increased maternal expression of the magnesium transporter TRPM7 may be associated with decreased glucose tolerance in pregnant women.
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
(2022)
Article
Biochemistry & Molecular Biology
Md Atikur Rahman, Ahmad Humayan Kabir, Yowook Song, Sang-Hoon Lee, Mirza Hasanuzzaman, Ki-Won Lee
Summary: This study demonstrates that nitric oxide (NO) plays a crucial role in restoring iron (Fe) deficiency-induced growth inhibition and oxidative stress in alfalfa. By upregulating specific genes, NO helps alleviate the negative effects of Fe deficiency by inhibiting oxidative stress and enhancing antioxidant defense mechanisms.
Article
Biochemistry & Molecular Biology
Setareh Jamali Jaghdani, Peter Jahns, Merle Traenkner
Summary: This study investigated the impact of varying magnesium concentrations on barley photosynthetic efficiency and photoprotective responses, revealing that CO2 assimilation is more sensitive to reduction of tissue magnesium concentrations, while supply with lower magnesium concentrations induced photo oxidative stress.
Article
Chemistry, Physical
Yi-Ming Ju, Fang Huang, Xin Ding, Li-Bo Mao, Shu-Hong Yu
Summary: This study investigates the mechanism of Mg2+ ion-regulated mineralization of CaCO3 by tracing Mg isotope fractionation. The different mineralization pathways of CaCO3 under different Mg2+ ion concentrations are clarified, and the detailed regulatory role of Mg2+ ions at different stages of mineralization is proposed. These results provide important insights for finely controlling the phase of crystalline products.
Article
Chemistry, Physical
Yi-Ming Ju, Fang Huang, Xin Ding, Li-Bo Mao, Shu-Hong Yu
Summary: This study clarifies the different mineralization pathways of CaCO3 under different Mg2+ ion concentrations by tracing Mg isotope fractionation. The detailed regulatory role of Mg2+ ions at different stages of mineralization is proposed.
Article
Biochemistry & Molecular Biology
Mohammad Alnayef, Celymar Solis, Lana Shabala, Takaaki Ogura, Zhonghua Chen, Jayakumar Bose, Frans J. M. Maathuis, Gayatri Venkataraman, Keitaro Tanoi, Min Yu, Meixue Zhou, Tomoaki Horie, Sergey Shabala
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Yasunori Ichihashi, Yasuhiro Date, Amiu Shino, Tomoko Shimizu, Arisa Shibata, Kie Kumaishi, Fumiaki Funahashi, Kenji Wakayama, Kohei Yamazaki, Akio Umezawa, Takumi Sato, Makoto Kobayashi, Mayu Kamimura, Miyako Kusano, Fang-Sik Che, Martin O'Brien, Keitaro Tanoi, Makoto Hayashi, Ryuhei Nakamura, Ken Shirasu, Jun Kikuchi, Naoto Nihei
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Plant Sciences
Rie Shimizu-Inatsugi, Silvija Milosavljevic, Kentaro K. Shimizu, Gabriela Schaepman-Strub, Keitaro Tanoi, Yasuhiro Sato
Summary: Excessive metal ions in soil can be toxic to plant species, but can also aid in plant survival by providing elemental defense against herbivores and pathogens. This study compared the accumulation levels of zinc and cadmium in the allotetraploid A. kamchatica with its diploid progenitors, finding that A. kamchatica had intermediate levels of zinc accumulation and lower levels of cadmium compared to its progenitors. Field experiments showed that A. kamchatica had a lower level of elemental defense compared to the hyperaccumulator progenitor A. halleri, consistent with its lower metal accumulation levels.
PLANT SPECIES BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mohammad Arif Ashraf, Takashi Akihiro, Keita Ito, Sayaka Kumagai, Ryohei Sugita, Keitaro Tanoi, Abidur Rahman
Summary: The study identified ABC proteins ABCG33 and ABCG37 as facilitators of Cs+ influx, showing that they act redundantly and independently of the potassium uptake pathway. This research used a combinatorial approach to confirm the role of ABCG33 and ABCG37 as new Cs+ influx carriers.
Article
Plant Sciences
Daiki Shinozaki, Keitaro Tanoi, Kohki Yoshimoto
Summary: This study demonstrates the important role of autophagy in adapting to excess zinc stress in plants, with the distribution of Fe3+ from source leaves to sink leaves via autophagy promoting healthy growth under excess zinc.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Physics, Applied
Keisuke Kurita, Takuro Sakai, Nobuo Suzui, Yong-Gen Yin, Ryohei Sugita, Natsuko Kobayashi, Keitaro Tanoi, Naoki Kawachi
Summary: The radioisotope tracer imaging system developed in this study, Live-autoradiography, allows continuous visualization of radioisotope dynamics in living plants, showing element migration and accumulation in different parts of intact plants. The system demonstrated good linearity between image intensity and Cs-137 radioactivity and was successfully used to image Cs-137 dynamics in a soybean plant for four days, revealing accumulation in specific plant regions. This system can be valuable for studying plant physiological phenomena and quantitatively measuring radionuclides.
JAPANESE JOURNAL OF APPLIED PHYSICS
(2021)
Article
Geography, Physical
Tomoki Oda, Norifumi Hotta, Satoru Miura, Izuki Endo, Keitaro Tanoi, Chris S. Renschler, Nobuhito Ohte
Summary: This study observed the spatial distribution of Cs-137 in a deciduous forest hillslope in Fukushima and used a mass balance model to explain its formation. The results showed that Cs-137 activity concentration was significantly higher in the downslope riparian area than in the upslope ridge area, and transport through litter and sediment contributed minimally to the inventory.
EARTH SURFACE PROCESSES AND LANDFORMS
(2022)
Article
Plant Sciences
Shahin Imran, Masumi Oyama, Rie Horie, Natsuko Kobayashi, Alex Costa, Ryosuke Kumano, Chiho Hirata, Sen Thi Huong Tran, Maki Katsuhara, Keitaro Tanoi, Takayuki Kohchi, Kimitsune Ishizaki, Tomoaki Horie
Summary: This study investigates the structure and function of the high-affinity potassium transporter (MpHKT1) in the liverwort Marchantia polymorpha. The C-terminal hydrophilic domain of MpHKT1 is found to play a unique role in regulating transport activity and ion selectivity. Overexpression of MpHKT1 leads to higher Na+ accumulation, while phenotypes with truncated C-terminal domains are weaker.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Ryo Tabata, Takehiro Kamiya, Shunpei Imoto, Hana Tamura, Kumiko Ikuta, Michika Tabata, Tasuku Hirayama, Hironaka Tsukagoshi, Keitaro Tanoi, Takamasa Suzuki, Takushi Hachiya, Hitoshi Sakakibara
Summary: Plants have evolved sophisticated systemic processes to optimize their nutrient acquisition efficiency in heterogeneous soil environments. This study investigates the molecular basis of long-distance signaling in response to iron starvation and reveals the regulatory mechanism of iron homeostasis in plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Yuko Kurita, Satomi Kanno, Ryohei Sugita, Atsushi Hirose, Miwa Ohnishi, Ayumi Tezuka, Ayumi Deguchi, Kimitsune Ishizaki, Hidehiro Fukaki, Kei'ichi Baba, Atsushi J. Nagano, Keitaro Tanoi, Tomoko M. Nakanishi, Tetsuro Mimura
Summary: This study visualized the seasonal phosphorus re-translocation routes in plants using real-time radioisotope imaging and autoradiography. The results provide insight into the molecular mechanisms of phosphorus redistribution and offer a basis for further understanding plant growth strategies.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Yusaku Noda, Atsushi Hirose, Mayumi Wakazaki, Mayuko Sato, Kiminori Toyooka, Naoki Kawachi, Jun Furukawa, Keitaro Tanoi, Ken Naito
Summary: This research uncovers a unique salt tolerance mechanism in Vigna riukiuensis. It was observed that V. riukiuensis accumulates higher sodium levels in the leaves compared to its relative V. nakashimae, which suppresses sodium allocation to the leaves. Interestingly, starch granules were found in the chloroplasts of V. riukiuensis, and degrading leaf starch resulted in no sodium accumulation. SEM-EDX analysis confirmed the presence of sodium around the starch granules in V. riukiuensis chloroplasts.
JOURNAL OF PLANT RESEARCH
(2023)
Letter
Plant Sciences
Kenji Suetsugu, Ryohei Sugita, Akiko Yoshihara, Hidehito Okada, Kae Akita, Noriko Nagata, Keitaro Tanoi, Koichi Kobayashi
Article
Multidisciplinary Sciences
Yukari Nagatoshi, Kenta Ikazaki, Yasufumi Kobayashi, Nobuyuki Mizuno, Ryohei Sugita, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Natsuko I. Kobayashi, Keitaro Tanoi, Kenichiro Fujii, Junya Baba, Eri Ogiso-Tanaka, Masao Ishimoto, Yasuo Yasui, Tetsuji Oya, Yasunari Fujita
Summary: Drought significantly affects crop production even under mild conditions. In this study, the authors develop a ridge-induced mild drought system in the field and find that phosphate starvation response occurs before ABA response in early mild drought. Their observations in both the field and laboratory provide insights into the molecular response to mild drought in field-grown plants and the relationship between nutrition and drought stress response.
NATURE COMMUNICATIONS
(2023)
Article
Agronomy
Yusaku Noda, Ryohei Sugita, Atsushi Hirose, Naoki Kawachi, Keitaro Tanoi, Jun Furukawa, Ken Naito
Summary: By studying different species in the Vigna genus, it was found that different germplasms have different mechanisms in Na+ allocation, which can be accumulated in a single crop through breeding or genetic engineering techniques.
Article
Biochemistry & Molecular Biology
Xiaoyu Yang, Natsuko Kobayashi, Yoshiki Hayashi, Koichi Ito, Yoshitaka Moriwaki, Tohru Terada, Kentaro Shimizu, Motoyuki Hattori, Ren Iwata, Hisashi Suzuki, Tomoko M. Nakanishi, Keitaro Tanoi
Summary: This study investigates the significance of the conserved GMN motif in the Arabidopsis thaliana AtMRS2-1 transporter for magnesium transport, using structural prediction and mutagenesis experiments.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)