Article
Electrochemistry
Sinem Evli, Deniz Aktas Uygun
Summary: This study developed a biosensor using laccase-immobilized L-cysteine functionalized-silver-coated magnetic Fe3O4 nanoparticles modified screen-printed carbon electrodes to detect catechol in water. The biosensor showed electrocatalytic activity for catechol and its analytical performance was optimized. The biosensor had a linear catalytic current for catechol concentration and demonstrated selective detection without interference from other substrates and matrix effect. This biosensor is a viable strategy for fast and cost-effective analysis of phenolic pollutants in environmental samples.
JOURNAL OF APPLIED ELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Olha Demkiv, Galina Gayda, Nataliya Stasyuk, Olena Brahinetz, Mykhailo Gonchar, Marina Nisnevitch
Summary: This article describes the development of amperometric biosensors based on laccase and redox-active micro/nanoparticles. It was found that laccase/CuCo/GE exhibited the highest sensitivity to catechol, suggesting potential applications of these sensors for the analysis of phenolic derivatives.
Article
Chemistry, Analytical
Sinem Evli, Deniz Aktas Uygun
Summary: One-dimensional Ni/Au/PPy-COOH nanowires with multiple segments were synthesized and their smooth surfaces and magnetic properties were investigated. These nanowires were used to modify the electrode surface and as a micro-environment for Trametes versicolor laccase. The biosensor based on this design demonstrated high sensitivity, excellent selectivity, wide linear range, and low LOD for the detection of dopamine in biological samples.
Article
Electrochemistry
Carina Figueiredo, Andrea Garcia-Ortega, Tanushree Mandal, Anna Lielpetere, Fadia Cervantes, Denise Demurtas, Edmond Magner, Francisco J. Plou, Wolfgang Schuhmann, Donal Leech, Marcos Pita, Antonio L. De Lacey
Summary: A developed amperometric biosensor utilizing osmium-complex modified redox polymers and galactose oxidase for determining galactose levels in dairy products demonstrated rapid and accurate measurement of galactose concentration under aerobic conditions. The optimized sensor provided maximum electrocatalytic response unaffected by the presence of oxygen, indicating efficient wiring of the enzyme. Validation of the biosensor results was conducted with a high-performance anion-exchange chromatography coupled with pulsed amperometric detection method.
ELECTROCHIMICA ACTA
(2023)
Article
Electrochemistry
Sandra Jose, Ditto Abraham Thadathil, Munmun Ghosh, Anitha Varghese
Summary: The uncontrolled release of harmful endocrine disruptors (EDs) is causing serious damage to human and animal health. A biosensor called Lac/PAA/CFP, which immobilizes laccase over electropolymerized poly anthranilic acid on a carbon fiber paper (CFP) electrode, was developed for the detection of p-nonylphenol (PNP), a persistent phenolic endocrine disruptor and a harmful eco-toxic pollutant. The biosensor showed good selectivity and a low limit of detection for PNP in real water samples.
ELECTROCHIMICA ACTA
(2023)
Article
Engineering, Chemical
Shuyan Ma, Chenjie Wei, Hong Jiang, Zhili Chen, Zhikang Xu, Xiaojun Huang
Summary: In this study, a biomimetic catalysis strategy was employed to achieve the directional deposition of dopamine on the target surface, leading to the successful preparation of a laccase-dopamine coating on biomimetic catalytic membranes with high catalytic activity and stability.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2022)
Article
Chemistry, Physical
Olha Demkiv, Nataliya Stasyuk, Galina Gayda, Mykhailo Gonchar
Summary: Nanozymes with excellent activity were synthesized and used for developing amperometric sensors for adrenaline analysis, showing higher sensitivity and lower detection limit compared to natural enzymes.
Article
Environmental Sciences
Mahsa Masjoudi, Mitra Golgoli, Zahra Ghobadi Nejad, Sadegh Sadeghzadeh, Seyed Mehdi Borghei
Summary: In this study, immobilized laccase was used for the removal of pharmaceutical micropollutants in water and wastewater, with remarkable activity and activity recovery on PVDF/MWCNT membranes. The immobilized laccase showed improved operational and thermal stability compared to free laccase. The findings suggest that immobilized laccase is a promising catalyst for large-scale water and wastewater treatment, achieving high removal efficiencies in a mini-membrane reactor.
Article
Environmental Sciences
Jenet George, Abdullah K. Alanazi, P. Senthil Kumar, Swethaa Venkataraman, Devi Sri Rajendran, Jothyswarupha Krishnakumar Athilakshmi, Isita Singh, Ishani Singh, Pramit Sen, Maheswari Purushothaman, Palanisamy Athiyaman Balakumaran, Vinoth Kumar Vaidyanathan, Hala M. Abo-Dief
Summary: A biocatalytic membrane incorporating superparamagnetic iron oxide nanoparticles showed better performance in the removal of toxic pentachlorophenol compared to free enzymes. The immobilized laccase on the membrane exhibited higher stability and activity recovery. The use of mediators, particularly veratryl alcohol, further enhanced the removal efficacy of the immobilized laccase. The study highlights the potential of the PEES/PMVEAMA/lac membrane system for pollutant removal in a membrane bioreactor.
Article
Chemistry, Analytical
Sinem Evli, Deniz Aktas Uygun
Summary: In this study, 2-dimensional graphene oxide-titanium disulfide (GO/TiS2) nanolayers were synthesized and investigated for their surface and size properties using transmission electron microscopy (TEM) technique. These nanolayers were used to modify the screen-printed electrode surface and applied as a micro-medium for Trametes versicolor laccase. The resulting enzyme biosensor demonstrated a wide linear range, low detection limit, high selectivity, and excellent performance in real food samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Biophysics
Van Chinh Hoang, Atefeh Shafaat, Skaidre Jankovskaja, Vincent G. Gomes, Tautgirdas Ruzgas
Summary: By evaluating biosensors in Franz cell setup, opportunities and obstacles can be better understood to avoid issues during testing in animals and humans.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
F. H. Cincotto, J. Oliveira Fernandes, A. C. O. Travassos, D. Y. Tiba, A. M. A. B. Medeiros, R. E. Santelli, A. Sanchez, R. Villalonga, J. M. Pingaron, T. C. Canevari
Summary: An electrochemical biosensor based on a hybrid nanostructure of polypyrrole-3-carboxylic acid/Sb2O5/reduced graphene oxide and laccase enzyme was developed for the determination of bisphenol A. The biosensor showed excellent sensitivity, selectivity, and recovery rate in real samples.
Article
Engineering, Environmental
Xiaoxuan Lou, Fangke Zhi, Xiaoyuan Sun, Fang Wang, Xiaohong Hou, Chongning Lv, Qi Hu
Summary: In this study, a method of co-immobilizing laccase-mediator system (LMS) by metal-organic frameworks (MOFs) was developed, where laccase and 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic Acid (ABTS) were immobilized on polyethylene terephthalate (PET).This method improved the degradation efficiency and recyclability of laccase.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Electrochemistry
K. Lakshmi Narayanan, J. Kavitha, Ramu Usha Rani, Michael E. G. Lyons, Lakshmanan Rajendran
Summary: This paper discusses the theoretical model of an amperometric glucose biosensor, in which the glucose oxidase enzyme is immobilized in conducting polypyrrole. A new approach using the Taylor method is used to solve the coupled nonlinear reaction diffusion equations. A comparison between numerical simulation and analytical approximation shows agreement between the results.
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
(2022)
Article
Polymer Science
Ancuta Dinu, Constantin Apetrei
Summary: This study presents the development of a new enzyme sensor for the quantification of tyrosine in pharmaceuticals. The enzyme sensor showed a low limit of detection and high sensitivity, and was validated using infrared spectrometry on pharmaceuticals from different manufacturers.
Article
Biotechnology & Applied Microbiology
Piyush Chandna, Shanmugam Mayilraj, Ramesh Chander Kuhad
ANNALS OF MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Kailash N. Bhardwaj, Kavish Kumar Jain, Sandeep Kumar, Ramesh Chander Kuhad
INDIAN JOURNAL OF MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Rishi Gupta, Girija Mehta, Ramesh Chander Kuhad
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Kavish Kumar Jain, Sandeep Kumar, Deepa Deswal, Ramesh Chander Kuhad
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2017)
Review
Green & Sustainable Science & Technology
Ramesh Chander Kuhad, Deepa Deswal, Sonia Sharma, Abhishek Bhattacharya, Kavish Kumar Jain, Amandeep Kaur, Brett I. Pletschke, Ajay Singh, Matti Karp
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2016)
Review
Food Science & Technology
Tapati Bhanja Dey, Subhojit Chakraborty, Kavish Kr. Jain, Abha Sharma, Ramesh Chander Kuhad
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2016)
Article
Energy & Fuels
Siddhartha Pal, Shereena Joy, Pramod Kumbhar, Kalpana D. Trimukhe, Rishi Gupta, Ramesh C. Kuhad, Anjani J. Varma, Sasisanker Padmanabhan
BIOMASS CONVERSION AND BIOREFINERY
(2017)
Article
Biotechnology & Applied Microbiology
Hemansi, Rishi Gupta, Ramesh Chander Kuhad, Jitendra Kumar Saini
BIOCHEMICAL ENGINEERING JOURNAL
(2018)
Article
Biochemistry & Molecular Biology
Kavish Kumar Jain, Sandeep Kumar, Kailash N. Bhardwaj, Ramesh Chander Kuhad
MOLECULAR BIOTECHNOLOGY
(2018)
Review
Biotechnology & Applied Microbiology
Abha Sharma, Kavish Kumar Jain, Arti Jain, Mazahir Kidwai, R. C. Kuhad
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Abha Sharma, Kavish Kumar Jain, Anita Srivastava, Bhuvnesh Shrivastava, Vasanta Vadde Thakur, R. K. Jain, R. C. Kuhad
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2019)
Article
Energy & Fuels
Amandeep Kaur, Ramesh Chander Kuhad
BIOENERGY RESEARCH
(2019)
Article
Engineering, Environmental
Rishi Gupta, Hemansi, Sheenu Gautam, Rishikesh Shukla, Ramesh Chander Kuhad
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2017)
Article
Microbiology
Shashwat Malhotra, Seema Singh, Neha Rana, Shilpi Tomar, Priyanka Bhatnagar, Mohit Gupta, Suraj K. Singh, Brajendra K. Singh, Anil K. Chhillar, Ashok K. Prasad, Christophe Len, Pradeep Kumar, Kailash C. Gupta, Anjani J. Varma, Ramesh C. Kuhad, Gainda L. Sharma, Virinder S. Parmar, Nigel G. J. Richards
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2017)
Article
Agriculture, Multidisciplinary
Garima Tiwari, Abhishek Sharma, Manu Dalela, Rishi Gupta, Satyawati Sharma, Ramesh Chander Kuhad
INDIAN JOURNAL OF AGRICULTURAL SCIENCES
(2017)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)
