Article
Chemistry, Analytical
Shuo Meng, Dongmiao Qin, Yusheng Wu, Guichun Mo, Xiaohua Jiang, Biyang Deng
Summary: Researchers prepared a composite material MnCO3/poly(diallyl dimethyl ammonium chloride) (PDDA)/Ag with excellent electrochemiluminescence (ECL) performance and high biocompatibility by adding MnCO3 and PDDA to silver nanoparticles (AgNPs). MnCO3 and Au@SiO(2)NPs were used as ECL donors and acceptors, respectively, establishing an effective ECL-resonance energy transfer (RET) sensing platform. The system showed good response to the concentration of A beta(42) in serum samples.
Article
Chemistry, Multidisciplinary
Ewelina Stefaniak, Elena Atrian-Blasco, Wojciech Goch, Laurent Sabater, Christelle Hureau, Wojciech Bal
Summary: This study investigated the effect of Cu-II on the aggregation of A beta(1-40) and A beta(4-40). It was found that substoichiometric Cu-II concentrations accelerated aggregation, while superstoichiometric Cu-II inhibited fibril formation. The interaction between Cu-II, A beta(4-40), and A beta(1-40) resulted in significant changes in the aggregation process.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Peter R. Christenson, Manci Li, Gage Rowden, Peter A. Larsen, Sang-Hyun Oh
Summary: Misfolded proteins associated with neurodegenerative diseases can accumulate in tissues or circulate in biological fluids before the onset of clinical symptoms, making them ideal diagnostic targets. Adding nanoparticles to the RT-QuIC assay improves its speed and sensitivity, which is crucial for diagnostic application.
Article
Nanoscience & Nanotechnology
Devi Wahyuningtyas, Wen-Hao Chen, Ruei-Yu He, Yung-An Huang, Chia-Kang Tsao, Yu-Jung He, Chu-Yi Yu, Po-Chao Lu, Yu-Cai Chen, Sheng-Hann Wang, Ka Chon Ng, Bryan Po-Wen Chen, Pei-Kuen Wei, Jiun-Jie Shie, Chun-Hong Kuo, Y. Henry Sun, Joseph Jen-Tse Huang
Summary: The researchers designed and synthesized a polyglutamine-specific gold nanoparticle complex that targeted mHtt and alleviated its toxicity. The complex was able to disassociate fibrillary aggregates from polyQ peptides and reduce beta-sheet content in a concentration-dependent manner. Additionally, the complex was shown to penetrate cells, bind to cytosolic mHtt proteins, dissociate mHtt inclusions, reduce mHtt oligomers, and ameliorate mHtt-induced toxicity, suggesting potential therapeutic strategies against Huntington's disease.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Guiyue Zheng, Shenglan Hu, Dongmiao Qin, Chunlian Nong, Lijuan Yang, Biyang Deng
Summary: This study presents an immunosensor for measuring A beta(42) levels using aggregation-induced enhanced electrochemiluminescence. The immunosensor utilizes metal-organic gels as substrates and demonstrates high sensitivity and stability.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Rong Tan, Yuhong Liu, Yueju Wang, Huiling Li, Yifeng Tu
Summary: A new label-free sensor based on luminol electrochemiluminescence (ECL) was developed using a close-packed monolayered-SiO2 array with gold (Au) nanoparticles (NPs) as the basal electrode. The ECL signal was amplified by the quasiphotonic crystal structure of SiO2 NPs and by the catalytic activity of Au NPs. The sensor showed a 64-fold-intensified ECL signal and served as a substrate for an aptasensor with high sensitivity and a low limit of detection of 5 fM for Afi.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Medicine, Research & Experimental
Hao Yu, Jie Wu
Summary: Amyloid-beta accumulation is a key feature of Alzheimer's disease, with both neurotoxic and potentially protective effects. Understanding the role of Aβ in depth will provide a broader perspective on Alzheimer's disease treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Nir Salinas, Einav Tayeb-Fligelman, Massimo D. Sammito, Daniel Bloch, Raz Jelinek, Dror Noy, Isabel Uson, Meytal Landau
Summary: The study found that two subtypes of antimicrobial peptide uperin 3.5 can form functional amyloid fibrils, which has important physiological implications. Uperin 3.5 mainly forms cross-beta fibrils in the absence of lipids, but helical fibril formation is induced by bacterial cells or membrane mimetics, leading to membrane damage and cell death.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Chyi Wei Chung, Gabriele S. Kaminski Schierle
Summary: Temperature is a crucial factor influencing biological processes in living cells, and intracellular temperature mapping can provide valuable insights into thermodynamic properties and cellular behavior. This review focuses on various thermometry systems, particularly those utilizing fluorescence-based techniques, discussing findings from intracellular measurements and highlighting discrepancies in current thermodynamic understanding related to mitochondrial thermogenesis and nuclear-cytoplasmic temperature differences. Additionally, intracellular thermometry is proposed as a tool for quantifying the energy flow and costs associated with A beta 42 aggregation in Alzheimer's disease.
Article
Biochemistry & Molecular Biology
Mohammad Khavani, Aliyeh Mehranfar, Mohammad R. K. Mofrad
Summary: The interactions between 10 short peptides and gold nanoparticles of different diameters have been studied using molecular dynamics simulations. The results show that gold nanoparticles significantly affect the stability and conformational properties of the peptides. The size of the gold nanoparticles and the amino acid sequences of the peptides play important roles in the stability of the peptide-gold nanoparticle complexes. The study provides new insight into peptide interaction with gold nanoparticles from a molecular viewpoint, which can be valuable for designing biomaterials based on peptides and gold nanoparticles.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Francesco Tavanti, Alfonso Pedone, Maria Cristina Menziani
Summary: This study demonstrated through simulations how gold nanoparticles adsorb amyloid-beta monomers, reducing their tendency to form mature amyloid fibrils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Jingjun Lyu, Xiaobing Long, Tuxiu Xie, Guijun Jiang, Jie Jiang, Lu Ye, Qiongzi Li
Summary: This study examined the impact of CuO nanoparticles on the neurotoxicity of alpha-synuclein oligomeric structures, finding that CuO nanoparticles can promote the fibrillation process of alpha-synuclein and increase cellular toxicity.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Multidisciplinary
Yu Zhang, Mengke Su, Xingru Fang, Wenwen Huang, Hao Jiang, Qi Li, Nisar Hussain, Mao Ye, Honglin Liu, Weihong Tan
Summary: In this study, a novel nanometal surface energy transfer (NSET) nanoruler with a single-nucleobase resolution was proposed for in situ identification of aptamer-binding targets on living cell membrane surfaces. The nanoruler consisted of FAM-labeled aptamers and single-sized gold nanoparticle (GNP) antibody conjugates as a donor and an acceptor, respectively. Experimental evidence demonstrated the single nucleobase resolution of the nanoruler by molecular size, orientation, quenching nature, and other dye-GNP pairs. The nanoruler showed high reproducibility and precision for measuring molecule distance on living cell membranes at the nanometer level due to the use of only single-sized antibody-capped GNPs. The proposed strategy represents a solid step towards the wider application of optical-based rulers for observing molecular structural configuration and dynamic transitions on the membrane surface of living cells.
Article
Chemistry, Multidisciplinary
Ye Zhao, Shan Sun, Yan Lyu, Mengyao Gao, Hengwei Lin, Cheng Yang
Summary: A near-infrared fluorescent nanoprobe based on semiconducting polymer nanoparticles (SPNs) is developed for detecting senescence-associated beta-gal (SA-beta-gal). This probe can visualize SA-beta-gal in living cells due to its inherent lysosome-locating feature.
CHEMICAL COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Hariharan Moorthy, Lakshmi Priya Datta, Sourav Samanta, Thimmaiah Govindaraju
Summary: Alzheimer's disease is a major neurodegenerative disorder characterized by the misfolding and aggregation of β-amyloid protein, leading to neuronal death and cognitive impairment. A cyclic dipeptide-based copolymer has been developed and shown to protect neuronal cells by inhibiting Aβ42 aggregation and reducing amyloid toxicity. The copolymer also interacts with gold nanoparticles and polyoxometalate, forming novel nanocomposites that further enhance its protective effects.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Chengke Wang, Kun Wang, Zhenxin Wang
ANALYTICA CHIMICA ACTA
(2015)
Article
Chemistry, Analytical
Chengke Wang, Xiaoya Dong, Qian Liu, Kun Wang
ANALYTICA CHIMICA ACTA
(2015)
Article
Biochemical Research Methods
Chengke Wang, Zhenxin Wang
ANALYTICAL BIOCHEMISTRY
(2015)
Article
Chemistry, Analytical
Chengke Wang, Qingqing Wang, Rong Tan
Article
Biochemical Research Methods
Chengke Wang, Rong Tan, Jiangyu Li, Zexiang Zhang
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Wang Chengke, Tan Rong, Li Jiangyu, Zhang Zexiang
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2019)
Article
Chemistry, Multidisciplinary
Wang Chengke, Tan Rong, Li Libo, Liu Dong
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2019)
Article
Chemistry, Multidisciplinary
Chengke Wang, Xueli Wang, Hei-Nga Chan, Guifeng Liu, Zhenxin Wang, Hung-Wing Li, Man Shing Wong
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Chemistry, Analytical
Xueli Wang, Chengke Wang, Hei-Nga Chan, Iyaswamy Ashok, Senthil Kumar Krishnamoorthi, Min Li, Hung-Wing Li, Man Shing Wong
Summary: Novel near infrared emissive fluorophores exhibit strong selectivity towards Aβ oligomers and monomers, good biocompatibility and stability, and significant inhibitory effects on Aβ aggregation.
Article
Chemistry, Analytical
Chengke Wang, Jiangyu Li, Ping Wang
Summary: In this study, it was discovered that adjacent double-strand DNA can enhance the fluorescence intensity of the G-triplex-thioflavin T complex. By connecting the newly formed dsDNA with specific sequences, target genes and metal ions can be detected. A versatile 'turn-on' fluorescence platform was also developed for discriminating transgenic and non-transgenic crops, as well as detecting metal ions in water.
Article
Spectroscopy
Wang Cheng-ke, Zhang Ze-xiang, Huang Xiao-we, Zou Xiao-bo, Li Zhi-huang, Shi Ji-yong
SPECTROSCOPY AND SPECTRAL ANALYSIS
(2020)
Article
Chemistry, Analytical
Chengke Wang, Jiangyu Li, Rong Tan, Qingqing Wang, Zexiang Zhang
Article
Biochemical Research Methods
Chengke Wang, Chengen Wang, Qingqing Wang, Dan Chen
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Chengke Wang, Dan Chen, Qingqing Wang, Rong Tan
BIOSENSORS & BIOELECTRONICS
(2017)
Article
Chemistry, Analytical
Chengke Wang, Dan Chen, Qingqing Wang, Qiuxia Wang
ANALYTICAL SCIENCES
(2016)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)
