Journal
ANALYTICAL METHODS
Volume 2, Issue 4, Pages 393-396Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b9ay00301k
Keywords
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Funding
- Indian Veterinary Research Institute
- Agricultural and Processed Food Export Development Authority, Govt. of India
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A simple and sensitive liquid chromatographic (LC) method was developed for determination of carbaryl residue in buffalo meat samples. This method is based on a solid-phase extraction technique followed by high-performance liquid chromatography (HPLC)-photo-diode-array (PDA) detection. Meat samples (0.5 g) were deproteinized by adding acetonitrile followed by centrifugation and filtration. The analyte was separated on a reverse-phase (RP-C(18)) column using isocratic elution. Acetonitrile along with water appears to be an excellent extractant as recovery of the analyte in spiked sample at maximum residue level (MRL) was 98.5%, with coefficient of variation (CV) of 4.97%. The limit of detection (LOD) and limit of quantification (LOQ) of the method was 0.015 and 0.03 mu g g(-1), respectively. The linearity of the carbaryl was 0.9992. Excellent method repeatability and reproducibility were also observed by intra- and inter-day assay precision. For robustness, the method was employed to analyze 122 buffalo meat samples, and intensities for the insecticide were found to be unaffected by the sample matrices interference.
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