Article
Biotechnology & Applied Microbiology
Jia Zhang, Xiaolu Su, Yefei Wang, Xiaohang Wang, Shiqi Zhou, Hui Jia, Xiaoyan Jing, Yanhai Gong, Jichao Wang, Jian Xu
Summary: Improved Single-cell Genome Amplification (iSGA) overcomes the low amplification efficiency, incomplete and uneven genome amplification issues in single-cell sequencing workflows through phi29 DNA polymerase engineering and process engineering. The developed HotJa Phi29 DNA Polymerase shows significantly better coverage (99.75%) at higher temperatures and achieves high single-cell genome amplification ability and coverage (93.59%) for commercial probiotic samples. iSGA is more efficient and cost-effective than the commercial Thermo Scientific EquiPhi29 DNA Polymerase, promising its broad applications in large-scale single-cell sequencing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
M. S. Goryunova, V. K. Arzhanik, S. K. Zavriev, D. Y. Ryazantsev
Summary: RCA-based detection methods offer exceptional sensitivity for a wide range of targets in molecular biology. The utilization of fluorescently labeled nucleotide analogs in RCA detection shows promise, with sulfo-cyanine3-dUTP demonstrating high incorporation effectiveness.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Mengni Sun, Na Ma, Hanxing Shi, Ling-Zhi Cheong, Wenge Yang, Zhaohui Qiao
Summary: A multivalent aptamer based on hybridization chain reaction (HCR) was designed as an effective signal amplifier for the sensitive detection of Salmonella. The multi-Apt exhibited a 33-fold enhancement in binding affinity and faster reaction kinetics compared to the monovalent aptamer (mono-Apt). By utilizing the HCR scaffold as a signal carrier, the proposed HCR-based multi-Apt amplifier could detect Salmonella as low as 7 cfu mL-1 with a broad detection range of 10 to 107 cfu mL-1. It also demonstrated excellent specificity against target bacteria and performed well on the detection of spiked food samples, showing great potential in practical Salmonella detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Zi-Tao Zhong, Ghazala Ashraf, Wei Chen, Lai-Bo Song, Shu-Jie Zhang, Bo Liu, Yuan-Di Zhao
Summary: Duplex-specific nuclease (DSN) is a promising tool for bioanalysis due to its unique capability to cleave DNA within duplexes. This study proposes a new sensing strategy by coupling DSN with DNA aptamer sequence for diverse analytes detection with signal amplification and cycling. The method shows satisfactory performance in detecting adenosine triphosphate, thrombin, and alpha-fetoprotein.
MICROCHEMICAL JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Minhyuk Lee, Seonhye Shin, Sungjee Kim, Nokyoung Park
Summary: Aptamers, as promising bio-recognition elements, have advantages such as specificity, high affinity, and easy synthesis. Fluorescent aptasensors are widely used in biological applications due to their high sensitivity, simple detection process, and low cost.
Article
Biochemistry & Molecular Biology
Li Li, Zhengbo Chen
Summary: In this study, a high specificity, flexibility, and simplicity aptasensor strategy was designed using a triblock DNA probe. It was found that the polyA fragment could assemble on the gold electrode surface via polyA instead of traditional Au-S bonds, leading to improved hybridization stability. The electrochemical aptasensor showed good repeatability, stability, and specificity.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Yusi Li, Xia Li, Fang Yang, Ruo Yuan, Yun Xiang
Summary: This study presents an aptamer-based method for highly sensitive detection of PDGF-BB in human serums, utilizing target-induced activation of DNA polymerase activity for recycling amplification cascades. By triggering the release of an active polymerase, the method achieved substantial fluorescence magnification and a low detection limit of 5.1 pM. The potential applicability for real samples was verified with diluted human serums, demonstrating the specificity and selectivity of the aptamer.
Article
Chemistry, Analytical
Yu Muto, Gen Hirao, Tamotsu Zako
Summary: The study presented a AuNP-based signal-amplifying sandwich biosensor for detecting target molecules without the need for conformational changes in ligands. Thrombin was used as a model target, triggering an extension reaction via hybridization of two probes and subsequently RNA synthesis initiation. The amplified RNA drives aggregation or dispersion of the AuNPs, leading to observable color differences in the solution. This detection system can be universally applied to a wide range of ligands due to its ligand-independent design.
Article
Chemistry, Analytical
Yong-Qiang He, Ying Chen, Xian-Zhu Meng, Hong -Chao Yi, Hui-Wen Gu, Xiao-Li Yin
Summary: A visual and smartphone-integrated aptasensor for rapid Staphylococcus aureus determination was developed using Exo III-assisted recycling and hemin/G-quadruplex DNAzymes for signal amplification. The assay quantified the released DNAzymes via Exo III-assisted recycling reaction and analyzed them with a smartphone and UV-Vis spectrophotometer. This method exhibited good analytical performance, low cost, and portability, making it a rapid point-of-care test method with great potential in food safety control.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biotechnology & Applied Microbiology
Yunsu Ma, Yuan Wang, Yongjie Liu, Lujia Shi, Dongzhi Yang
Summary: A multi-carbon dots and aptamer-based signal amplification ratiometric fluorescence probe was developed for the detection of protein tyrosine kinase 7. The probe was successfully applied to PTK 7 detection in MCF-7 cells and human serum with promising results, indicating its potential for clinical use.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Chengyi Hong, Xiaoxia Zhang, Sishi Ye, Hongfen Yang, Zhiyong Huang, Dan Yang, Ren Cai, Weihong Tan
Summary: The study developed a highly selective and sensitive method for detecting TET, applicable for TET determination in animal-derived foods, with results consistent with commercial ELISA kits.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Shizhen He, Shanshan Yu, Ruomeng Li, Yingying Chen, Qing Wang, Yuqiu He, Xiaoqing Liu, Fuan Wang
Summary: This study develops an efficient non-enzymatic circuitry activation strategy to achieve high-fidelity microRNA imaging inside live cells. The catalytic DNA circuit can detect targets sensitively through multiply guaranteed molecular recognition and progressively accelerated signal amplification.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Ghazala Ashraf, Zi-Tao Zhong, Muhammad Asif, Ayesha Aziz, Laibo Song, Shujie Zhang, Bo Liu, Wei Chen, Yuan-Di Zhao
Summary: In this study, a novel approach has been proposed to extend the sensing application of duplex specific nuclease (DSN) by coupling RNA aptamer. The method achieves target detection through fluorescent signal enhancement by cleaving hybridized probes with DSN. The assay has a low detection limit and can be applied for real-time detection of thrombin release from live cells.
Review
Chemistry, Multidisciplinary
Yongxi Zhao, Xiaolei Zuo, Qian Li, Feng Chen, Yan-Ru Chen, Jinqi Deng, Da Han, Changlong Hao, Fujian Huang, Yanyi Huang, Guoliang Ke, Hua Kuang, Fan Li, Jiang Li, Min Li, Na Li, Zhenyu Lin, Dingbin Liu, Juewen Liu, Libing Liu, Xiaoguo Liu, Chunhua Lu, Fang Luo, Xiuhai Mao, Jiashu Sun, Bo Tang, Fei Wang, Jianbin Wang, Lihua Wang, Shu Wang, Lingling Wu, Zai-Sheng Wu, Fan Xia, Chuanlai Xu, Yang Yang, Bi-Feng Yuan, Quan Yuan, Chao Zhang, Zhi Zhu, Chaoyong Yang, Xiao-Bing Zhang, Huanghao Yang, Weihong Tan, Chunhai Fan
Summary: Nucleic acids are natural biopolymers that play central roles in storing, encoding, transmitting and expressing genetic information. The field of nucleic acids analysis has rapidly advanced with technological breakthroughs, leading to the development of new analysis methods. However, there are also challenges and further research needed in this area.
SCIENCE CHINA-CHEMISTRY
(2021)
Article
Chemistry, Analytical
Junyang Wang, Hongxia Li, Caiyi Du, Ying Li, Xinyue Ma, Chuanyu Yang, Wentao Xu, Chunyan Sun
Summary: A novel aptasensor for tobramycin was successfully constructed using hybridization chain reaction (HCR) and fluorescence synergism. The aptasensor showed low detection limit and could monitor tobramycin in a wide concentration range. The amplification of fluorescence signal was achieved through HCR and the released cDNA-FAM acted as an initiator to trigger the amplification. Graphene oxide effectively quenched the background signal, enabling sensitive and specific detection of tobramycin.