Journal
ANALYTICAL LETTERS
Volume 45, Issue 2-3, Pages 283-295Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2011.633186
Keywords
Molecularly imprinted; Piezoelectric quartz crystal (PQC); Quartz crystal microbalance (QCM); Staphylococcal enterotoxin B (SEB)
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Funding
- National Nature Science Foundation of China [30800915]
- National Science and Technology Supporting Program of China [2009BADB9B03-Z05]
- National High Technology R&D Program of China [2010AA06Z302]
- Science and Technology Program of Tianjin [09ZCKFSH02700]
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We fabricated a two-dimensional (2D) molecularly imprinted sol-gel thin film-coated quartz crystal microbalance (QCM) for the rapid detection of staphylococcal enterotoxin B (SEB) by combining organosilanes and the template protein SEB on the surface of piezoelectric quartz crystal (PQC) Au-electrode by in-situ immobilization. The detection process was monitored by the QCM's frequency shift (Delta f). The working range of this method was 1.0 x 10(-1)-1.0 x 10(3) mu g/mL. The detection limit was 6.1 ng/mL, which was lower than that of the PQC immunosensor, and the detection period was within 0.5 h. The reproducibility of the imprinted film-coated QCM was satisfactory due to no significant statistical difference (P > 0.05) in the rapid detection of SEB between intra-and inter-batch. The selectivity of the imprinted sol-gel film showed that it could discriminate the template molecule from its analogues and other guest molecules. Compared with immunochip, the imprinted film-coated QCM is more advantageous in terms of simplicity, rapidity, low cost, and sensitivity. Moreover, in real sample analysis, the recoveries of this method were 89.4-106.63%, which can be considered a favorable and applicable method for the rapid determination of SEB in real samples.
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