Journal
ANALYTICAL LETTERS
Volume 42, Issue 13, Pages 2096-2105Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710903082531
Keywords
Derivatization; electron ionization; gas chromatography; mass spectrometry; pinitol
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Funding
- Center for Science and Engineering Education at Pacific Northwest National Laboratory, Department of Energy, Office of Science
- U.S. Department of Energy [DE-AC05-76RLO 1830]
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Pinitol (D-3-O-methyl-chiro-inositol), a cyclitol, has a variety of roles in plant biology, and is being used as a nutritional supplement. These applications increase the importance of finding new methods for determining the pinitol content in plant tissues. A reliable method for the identification and quantitation of pinitol using trimethylsilyl imidazole (TMSI) derivatization and gas chromatography/mass spectrometry (GC/MS) was developed. One major ion fragment, m/z 260, was used to quantify pinitol in three plant species. Soybeans contained approximately 1.9mg/g wet weight pinitol while levels in sugar beets and snap beans were below detection limits.
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