Journal
ANALYTICAL LETTERS
Volume 42, Issue 5, Pages 790-806Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710802677175
Keywords
Alginate; enzyme immobilization; urea sensing
Categories
Funding
- Indian Council for Medical Research, New Delhi, India
- Department of Biotechnology, New Delhi, India
Ask authors/readers for more resources
A new absorbance-based enzymatic biosensor for determination of urea (in the range 0.01 to 6.7 mM) is described. Quantification using cresol red dye, immobilized in the nanofilm coatings assembled on alginate microspheres to immobilize the urease enzyme, has been accomplished using ratiometric absorbance measurements. The effect of salt concentration in polyelectrolyte nanofilms (on the stability of dye molecules) and buffer pH (on the enzyme stability) are reported. The results demonstrate excellent stability of sensing assay within alginate microspheres. Urea-sensing experiments demonstrate the potential to develop an optical urea sensor that is stable over a month.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available