Article
Agriculture, Dairy & Animal Science
Dawei Yao, Xin Zhao, Shuying Zhao, Hengbo Shi, Yi Ma, Jun Li
Summary: This study elucidated the transcriptional regulation of FABP3 in the goat mammary gland, showing that CREB1 is involved in the direct mechanism regulating FABP3 expression. The CRE2 site was found to be essential for CREB1-induced FABP3 transcriptional activity.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Qiuya He, Jun Luo, Jiao Wu, Zhuang Li, Weiwei Yao, Saige Zang, Huimin Niu
Summary: The role of ELOVL6 gene in the synthesis of long-chain saturated and monounsaturated fatty acids is important. Studies have shown that SREBP1 regulates the transcription of ELOVL6 through the SRE elements in the ELOVL6 promoter.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Ming Lyu, Fang Li, Xu Wang, Kun Xu, Shuang Sun
Summary: MicroRNA-mediated gene regulation is crucial for fatty acid metabolism and synthesis. The study revealed that miR-145 expression is higher in lactating mammary gland of dairy cows compared to the dry period. Knockout of miR-145 resulted in downregulation of fatty acid metabolism associated genes. In addition, miR-145 was found to target FOXO1 gene, which when silenced, promoted fatty acid metabolism and TAG synthesis in bovine mammary epithelial cells. These findings provide valuable insights into the molecular mechanisms for improving milk yield and quality.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Zhifei Zhang, Huibin Tian, Xiaoying Chen, Haiying Zhao, Wei Du, Huijie Gao, Jun Luo, Huiling Zheng
Summary: This study demonstrates the importance of the TPH1 gene in regulating calcium transportation and maintaining calcium homeostasis in GMEC. Knocking out the TPH1 gene leads to decreased 5-HT synthesis, intracellular calcium levels, and alters the expression of genes related to calcium transportation.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Peixin Jiao, Meimei Zhang, Ziwei Wang, Gege Liang, Xiaolai Xie, Yonggen Zhang, Zhi Chen, Qianming Jiang, Juan J. Loor
Summary: The fatty acid composition is crucial for the flavor and quality of goat milk. This study reveals that circ003429 plays a significant role in regulating lipid synthesis by inhibiting the action of miR-199a-3p on YAP1 mRNA abundance, thus affecting the synthesis of triglycerides and unsaturated fatty acids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
D. W. Yao, J. Ma, C. L. Yang, L. L. Chen, Q. Y. He, D. N. Coleman, T. Z. Wang, X. L. Jiang, J. Luo, Y. Ma, J. J. Loor
Summary: PTEN is a well-known tumor suppressor that plays a role in regulating cellular processes, including lipid metabolism. This study found that PTEN abundance decreased during peak lactation in goat mammary tissue. Overexpressing PTEN in isolated goat mammary epithelial cells led to downregulation of lipid metabolism-related genes and a shift towards lipolysis and fatty acid oxidation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Lu Zhu, Hongyun Jiao, Wenchang Gao, Lian Huang, Chenbo Shi, Fuhong Zhang, Jiao Wu, Jun Luo
Summary: It was found that miR-26a and miR-26b play a crucial role in lipid metabolism in goat mammary epithelial cells (GMECs), but their endogenous regulatory mechanism in fatty acid metabolism is uncertain. Using the CRISPR/Cas9 system with four sgRNAs, GMECs with simultaneous knockout of miR-26a and miR-26b were obtained. The knockout GMECs showed reduced levels of triglyceride, cholesterol, lipid droplets, and unsaturated fatty acid (UFA), as well as decreased expression of genes related to fatty acid metabolism, while the expression of the miR-26 target gene INSIG1 was significantly increased. Interestingly, the UFA content in the simultaneous knockout GMECs was significantly lower compared to wild-type GMECs and cells with miR-26a or miR-26b knockout alone. Restoring the expression of INSIG1 in the knockout cells led to the restoration of triglyceride, cholesterol, lipid droplets, and UFA levels. This study demonstrates that the knockout of miR-26a/b suppresses fatty acid desaturation by upregulating the target gene INSIG1, providing valuable methods and data for studying miRNA functions and regulating mammary fatty acid synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Tao Xie, Yinuo Liu, Huixian Lu, Ambreen Iqbal, Mengru Ruan, Ping Jiang, Haibin Yu, Jilun Meng, Zhihui Zhao
Summary: This study investigated the effect of the ASIP gene on lipid metabolism in bovine mammary epithelial cells. Knockout of the ASIP gene using CRISPR/Cas9 technology resulted in altered expression of lipid metabolism-related genes and increased synthesis of medium and long-chain fatty acids.
Article
Genetics & Heredity
Yue Zhang, Jie Zhou, Shuang Liu, Zhibin Ji
Summary: In this study, a lncRNA database for Laoshan dairy goats was established using Illumina/Solexa sequencing. It was found that the lncRNA LOC102188416 acts as a sponge for miR-143-3p and co-regulates the expression of MAPK1 in GMECs. The existence of the lncRNA LOC102188416/miR-143-3p/MAPK1 axis was confirmed.
Article
Agriculture, Dairy & Animal Science
Shujuan Liu, Heran Cao, Dan Guo, Yue Jiang, Hao Yin, Junru Zhu, Quyu Duan, Emeline Diane Mariam Seleh-Zo, Guang Li, Xiaopeng An, Binyun Cao
Summary: This study found that Pou2F3 can inhibit cell proliferation and induce apoptosis in MECs. In addition, silencing Pou2F3 alters the phosphorylation levels of proteins in the PI3K/AKT/mTOR signaling pathway, suggesting that Pou2F3 may serve as an upstream regulator of this pathway in MECs.
ANIMAL BIOTECHNOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
Jun Zhang, Lu Deng, Xia Zhang, Yangchun Cao, Mengmeng Li, Junhu Yao
Summary: Improving nitrogen utilization efficiency in dairy production systems is important for reducing feed costs and addressing environmental concerns. Supplementing a low protein content diet with essential amino acids (EAAs) has been widely used to enhance nitrogen efficiency. However, single EAA supplementation does not consider the complex interactions and responses among EAAs, leading researchers to explore the benefits of supplementing multiple EAAs. This review summarizes the literature supporting the advantages of complete EAA mixtures in improving milk protein yield (MPY) and highlights the mechanisms involved in mammary protein metabolism and milk protein synthesis (MPS).
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Shuang-feng Chu, Tian-qi Zhao, Abdelaziz Adam Idriss Arbab, Yi Yang, Zhi Chen, Zhang-ping Yang
Summary: Fat is an essential nutrient and basic metabolite for sustaining life, and milk is rich in fatty acids. MicroRNA (miRNA) and mRNA play a crucial role in regulating milk fat metabolism in mammary gland tissue. This study identified a key gene, TGFA, that regulates milk fat metabolism through transcriptome sequencing. The miR-140/TGFA pathway was found to inhibit milk fat metabolism and improve milk quality by genetic means.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Agriculture, Dairy & Animal Science
K. Nichols, J. Dijkstra, M. J. H. Breuer, S. Lemosquet, W. J. J. Gerrits, A. Bannink
Summary: This study investigated the response of mammary gland metabolism and whole-body glucose rate of appearance to different profiles of metabolizable protein composed of essential amino acids (EAA). The results showed that when the EAA profile of metabolizable protein was incomplete, the intramammary catabolism of group 2 amino acids increased to support milk component synthesis. Additionally, the glucose metabolism in both whole body and mammary gland was flexible in support of milk component synthesis, regardless of the absorptive EAA profile.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Zhi Chen, Qinyue Lu, Xinlong Zhang, Zhiyue Zhang, Xiang Cao, Kun Wang, Xiaotan Lu, Zhangping Yang, Juan J. Loor, Peixin Jiao
Summary: This study identified the expression levels of circRNAs in the mammary tissue of dairy goats and found that circ007071 was significantly up-regulated during peak lactation. Further investigation revealed that circ007071 regulates milk fat synthesis by interacting with miRNA, leading to the promotion of triglyceride and saturated fatty acid synthesis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
L. Bernard, Y. Chilliard, K. Hove, H. Volden, R. A. Inglingstad, M. Eknaes
Summary: In this study, adding lipid to the diet of dairy goats was found to affect milk fat and fatty acid composition. The expression of mammary genes in goats was more regulated by lactation stage than by the dietary treatments applied.
JOURNAL OF DAIRY SCIENCE
(2022)