Article
Multidisciplinary Sciences
Xiaobo Yan, Junjiang Yang, Xiaohui Dong, Beiping Tan, Shuang Zhang, Shuyan Chi, Qihui Yang, Hongyu Liu, Yuanzhi Yang
Summary: The study aimed to explore the optimum protein requirement of juvenile grouper. Results showed that as the protein level increased, there were changes in growth, feed utilization, and nutrient metabolism. The optimum protein level for juvenile grouper was found to be 521.84 g/kg.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yue-Xuan Wang, Sui-Feng Xu, Ye-Wen Wang, Yun-Xiang Jiang, Qi-Wei Qin, Shi-Na Wei
Summary: This study aimed to investigate the protective effect of curcumin against Singapore grouper iridovirus-induced intestinal damage in orange-spotted groupers. The results showed that curcumin significantly alleviated intestine damage, repaired intestinal barrier dysfunction, suppressed intestinal cells apoptosis and inflammatory response, protected intestinal cells from oxidative injury, and restored the disruption of the intestinal microbiota caused by the virus. These findings provide a theoretical basis for the use of curcumin in aquaculture to prevent SGIV-induced intestinal injury.
Article
Environmental Sciences
Huiling Zeng, Peifeng Zhang, Hengzhen Ye, Yuxiang Ji, Christer Hogstrand, Iain Green, Juan Xiao, Qiongyao Fu, Zhiqiang Guo
Summary: This study investigated the impact of waterborne Zn exposure on the energy metabolism in orange-spotted grouper embryos. The findings showed that Zn exposure affected the expression of genes involved in glucose metabolism pathways, suggesting a developmental stage-dependent Zn uptake and genotoxicity in fish embryos. Furthermore, the study highlights the potential of fish embryos as a biosystem for evaluating cytotoxicity/genotoxicity of waterborne metals in aquatic ecosystems.
ENVIRONMENTAL POLLUTION
(2021)
Article
Fisheries
Chun Hui Ai, Zong Xian Zhu, Dan Dan Huang, Gan Yang, Tong De Liu, Ying Bai, Xue Ying Liang, Ying Ying Xiong, Yi Long Lin, Hao Ran Lin, Shui Sheng Li, Jun Hong Xia
Summary: In this study, the genetic architecture of early growth-related traits in the orange-spotted grouper was dissected using a genomewide association study. A total of 11 significantly associated SNPs located on three chromosomes were identified, explaining a proportion of phenotypic variance ranging from 5.15% to 6.89% for body weight, body length, and body height.
Article
Fisheries
Yinghui Qin, Haixiang Liu, Peipei Zhang, Si Deng, Reng Qiu, Lunguang Yao
Summary: In this study, a STAT2 gene from orange-spotted grouper was cloned and characterized. The findings suggest that EcSTAT2 exerts antiviral activity against red-spotted grouper nervous necrosis virus through up-regulation of host interferon response.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Xinxin Shan, Tengfei Xu, Zhiyuan Ma, Xinhui Zhang, Zhiqiang Ruan, Jieming Chen, Qiong Shi, Xinxin You
Summary: This study aimed to improve the ammonia tolerance of groupers through genomic selection, predicting the genomic estimated breeding values using genome-wide loci and single nucleotide polymorphisms. Results showed that the method based on GWAS-informative SNPs performed better in cost-efficiency for genomic selection.
Article
Fisheries
Yongzhi Chen, Yun Liu, Qing Wang, Xilan Ma
Summary: This study discovered that long double-strand RNA can effectively inhibit red-spotted grouper nervous necrosis virus infection, providing a potential novel approach for RNA-guided immunity against RNA viruses in fish.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Honglin Han, Liqun Wang, Suifeng Xu, Shaowen Wang, Min Yang, Chengzong Han, Qiwei Qin, Shina Wei
Summary: In this study, Ec-MARCO was identified and characterized in the orange-spotted grouper, and its important role in regulating the immune response against SGIV infection was demonstrated.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Mengshi Sun, Siting Wu, Shaozhu Kang, Jiaming Liao, Luhao Zhang, Zhuqing Xu, Hong Chen, Linting Xu, Xin Zhang, Qiwei Qin, Jingguang Wei
Summary: This study found that the infection of red-spotted grouper nervous necrosis virus (RGNNV) induced the formation of stress granules (SGs) in grouper spleen cells. The SGs particles co-located with the classic SG marker protein eIF3 eta, and some SGs depolymerized under treatment with a translation inhibitor. Inhibition of the PERK kinase significantly inhibited the formation of SGs and decreased the phosphorylation of eIF2 alpha. Overexpression of G3BP1 in Epinephelus coioides inhibited the replication of RGNNV by up-regulating the interferon and inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Jinpeng Chen, Liqun Wang, Jianling Huang, Xinshuai Li, Lingfeng Guan, Qing Wang, Min Yang, Qiwei Qin
Summary: This study cloned and characterized a novel type of MHC-I genotype, named EcMHC-IA*01, which plays an important role in antiviral immune response and apoptosis in fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yuexuan Wang, Suifeng Xu, Yewen Wang, Xuchao Zhang, Jiatao Chen, Qiwei Qin, Shina Wei
Summary: This study found that curcumin, extracted from the traditional Chinese medicine turmeric, has various biological activities, including immunoenhancement, antioxidant, and antibacterial properties. The results showed that curcumin can enhance fish immunity, reduce mortality caused by viral infection, and repair liver injury caused by the virus. This provides a theoretical foundation for the functional development of curcumin in aquaculture.
Article
Fisheries
Xiong Li, Hongping Chen, Yihao Cen, Jiule Wang, Na Ni, Xueming Dan, Zequan Mo, Yanwei Li
Summary: This study identified three immunogenic immobilization antigen genes (CI-IA1, CI-IA2, and CI-IA8) from Cryptocaryon irritans. Vaccination with these recombinant antigens elicited a high immobilization titer and protected grouper against C. irritans infection. The findings suggest that these antigens have potential for vaccine development.
Article
Fisheries
Dapeng Yu, Guanjian Yang, Hongli Xia, Zhen Gan, Zhiwen Wang, Liqun Xia, Kevin W. H. Kwok, Jia Cai, Yishan Lu
Summary: The present study aimed to investigate the pathogenesis of DSS-induced enteritis in Orange-spotted grouper (Epinephelus coioides). Fish were challenged with 200 μl 3% DSS via oral irrigation and feeding, using an appropriate dosage based on the disease activity index. The results showed that DSS-induced inflammatory responses were closely associated with the expression of pro-inflammatory cytokines, NF-kappa B, and myeloperoxidase (MPO) activity. Histological examination and SEM analysis revealed severe intestinal lesions, inflammatory cell infiltration, and microvillus effacement. The injured intestinal villi gradually recovered during the experimental period of 18 days. These findings provide valuable insights into the pathogenesis of enteritis in farmed fish, contributing to the control of enteritis in aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Zhenyu Liang, Shuping Zhang, Zihong Zou, Jinze Li, Rimin Wu, Liqun Xia, Gang Shi, Jia Cai, Jufen Tang, Jichang Jian
Summary: In this study, the researchers characterized a BAG3 homolog from orange-spotted grouper and investigated its roles during viral infection. They found that BAG3 plays an important role in modulating virus-induced cell death, regulating the expression of interferon and inflammation-related factors, and influences the immune response of fish during viral infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Suifeng Xu, Yuexuan Wang, Chengzong Han, Yunxiang Jiang, Qiwei Qin, Shina Wei
Summary: The study revealed that cystatin F (CyF) plays a crucial role in fish virus infection by participating in innate immune responses. It inhibits the expression of viral genes and regulates apoptosis, as well as the production of inflammatory factors. CyF also modulates the activity of promoters related to interferon and NF-kappa B. These findings highlight the importance of CyF in DNA virus infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
