Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 16, Issue 10, Pages 23143-23164Publisher
MDPI
DOI: 10.3390/ijms161023143
Keywords
TAL; TALE; CRISPR/Cas; Cas9; dCas9; sgRNA; transcription; live imaging; locus-specific ChIP; enChIP
Funding
- Takeda Science Foundation
- Asahi Glass Foundation
- Uehara Memorial Foundation
- Kurata Memorial Hitachi Science and Technology Foundation
- Ministry of Education, Culture, Sports, Science and Technology of Japan [15K06895, 25118512, 15H01354, 15H04329, 26650059]
- Grants-in-Aid for Scientific Research [15H04329, 26650059, 25118512, 15H01354, 15K06895] Funding Source: KAKEN
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Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species. The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner. In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
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