Journal
ANALYTICAL BIOCHEMISTRY
Volume 455, Issue -, Pages 48-54Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2014.03.019
Keywords
Metabolomics; Sample preparation; Orthogonal chromatography; Mass spectrometry; Dried plasma spot
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We describe a simple protocol for the preparation and orthogonal hydrophobic/hydrophilic LC-MS/MS analysis of mouse and human plasma samples, which enables the untargeted (shotgun) or targeted profiling of hydrophilic, amphipathic, and hydrophobic constituents of plasma metabolome. The protocol is rapid, efficient, and reliable, and offers several advantages compared to current procedures. When applied to a training set of human plasma samples, the protocol allowed for the rapid acquisition of full LogP metabolic profiles in plasma samples obtained from cognitively healthy human subjects and age-matched subjects with mild cognitive impairment or Alzheimer's disease (n = 15 each). Targeted analyses confirmed these findings, which are consistent with data previously published by other groups. (c) 2014 The Authors. Published by Elsevier Inc.
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