Journal
ANALYTICAL BIOCHEMISTRY
Volume 451, Issue -, Pages 45-47Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2014.02.002
Keywords
MALDI-TOF MS; Thin-layer chromatography; Phospholipids; Matrix background
Funding
- German Research Council [DFG Schi 476/12-1, SFB 1052/B6, FU 771/1-2]
- Virtual Liver Network [BMBF 0315735]
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Normal phase thin-layer chromatography (NP TLC) is an established method of (phospho)lipid analysis. The determination of the fatty acyl composition is, however, a more challenging task by NP TLC. The direct coupling of TLC separation with mass spectrometric detection (e.g., matrix-assisted laser desorption/ionization mass spectrometry, MALDI MS), however, enables a detailed characterization of complex lipid mixtures. Here we show that the thickness of the silica gel layer has a considerable effect on the quality of the mass spectra recorded directly from the TLC plate. In particular, the intensity of the matrix background signals can be reduced if thinner TLC layers are used. (C) 2014 Elsevier Inc. All rights reserved.
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