Journal
ANALYTICAL BIOCHEMISTRY
Volume 462, Issue -, Pages 10-12Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2014.05.015
Keywords
Aptamer; Thrombin; Signal amplification; ELISA
Funding
- Basic Science Program through the National Research Foundation of Korea (KRF) - Ministry of Science, ICT and Future Planning (MSIP) [2011-0021956]
- Ministry of Education (MOE) [2012-001680]
- National Research Foundation of Korea [2011-0021956] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A colorimetric sandwich-type assay based on enzyme-linked aptamer assay has been developed for the fast and sensitive detection of as low as 25 fM of thrombin with high linearity. Aptamer-immobilized glass was used to capture the target analyte, whereas a second aptamer, functionalized with horseradish peroxidase (HRP), was employed for the conventional 3,5,3',5'-tetramethylbenzidine (TMB)-based colorimetric detection. Without the troublesome antibody requirement of the conventional enzyme-linked immunosorbent assay (ELISA), as low as 25 fM of thrombin could be rapidly and reproducibly detected. This assay has superior, or at least equal, recovery and accuracy to that of conventional antibody-based ELISA. (C) 2014 Elsevier Inc. All rights reserved.
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