Article
Chemistry, Analytical
Inseon Lee, Seok-Joon Kwon, Mirco Sorci, Peter S. Heeger, Jonathan S. Dordick
Summary: This study utilized CRISPR technology and immunoassay methods to detect the protein CXCL9 in the urine of kidney transplant recipients, demonstrating improved sensitivity and accuracy of detection through enhanced DNA barcode system and Cas12a enzyme trans-cleavage activity, while showing high selectivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Meng-Yuan Yang, Fan Wu, Feng Fang, Hao Yang, Jing-Fan Zhang, Guo-Dong Chen, Lian-Yue Yang
Summary: EGFL7 is significantly elevated in patients with early HCC and shows higher sensitivity than AFP in diagnosing early HCC. It also has higher accuracy and sensitivity than AFP in distinguishing early HCC from patients with chronic liver disease.
Review
Urology & Nephrology
Charlotte Cortvrindt, Reinhart Speeckaert, Joris R. Delanghe, Marijn M. Speeckaert
Summary: Urinary EGF levels are associated with kidney diseases and can be used in conjunction with other biomarkers for risk assessment and diagnosis of CKD.
AMERICAN JOURNAL OF NEPHROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Christine Legay, Sophie Doublier, Sylvie Babajko, Jean-Marc Ricort
Summary: This study found that protein kinase D1 (PKD1) plays a crucial role in regulating the tumor growth-promoting epidermal growth factor (EGF) signaling pathway in breast cancer. The expression level of PKD1 is closely associated with the extent of Akt and ERK phosphorylation induced by EGF.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Junya Ning, Yingnan Ye, Dechao Bu, Gang Zhao, Tianqiang Song, Pengpeng Liu, Wenwen Yu, Hailong Wang, Hui Li, Xiubao Ren, Guoguang Ying, Yi Zhao, Jinpu Yu
Summary: The imbalance of TGF-β1/BMP-7 pathways is associated with aggressive features and poor outcomes in HCC. Modulating this imbalance through miR-17-92 cluster and ACVR1 inhibitors could be a potential therapeutic strategy for HCC.
Article
Biotechnology & Applied Microbiology
Li-Hao Deng, Hui Zhao, Li-Ping Bai, Jun Xie, Kai Liu, Feng Yan
Summary: The study reveals that linc00467 is overexpressed in gastric cancer and promotes the proliferation, migration, and invasion of GC cells by directly regulating the miR-7-5p and EGFR signaling pathway.
Article
Biology
Gian Paolo Caviglia, Maria Lorena Abate, Emanuela Rolle, Patrizia Carucci, Angelo Armandi, Chiara Rosso, Antonella Olivero, Davide Giuseppe Ribaldone, Francesco Tandoi, Giorgio Maria Saracco, Alessia Ciancio, Elisabetta Bugianesi, Silvia Gaia
Summary: The study found that serum ERBB3 levels may serve as a potential diagnostic and prognostic biomarker in patients with early hepatocellular carcinoma infected with hepatitis C virus, assisting in tailoring more personalized treatment strategies.
Article
Gastroenterology & Hepatology
David M. Hughes, Sarah Berhane, C. A. Emily de Groot, Hidenori Toyoda, Toshifumi Tada, Takashi Kumada, Shinji Satomura, Naoshi Nishida, Masatoshi Kudo, Toru Kimura, Yukio Osaki, Ruwanthi Kolamunage-Dona, Ruben Amoros, Tom Bird, Marta Garcia-Finana, Philip Johnson
Summary: This study developed and validated a model to assess the risk of hepatocellular carcinoma (HCC) in patients with chronic liver disease based on longitudinal analysis of serum alpha-fetoprotein (AFP) measurements. The model showed good performance in identifying patients who developed HCC, allowing for classification into high-risk and low-risk groups. This model could help in selecting patients for surveillance and improving detection of HCC.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Gastroenterology & Hepatology
Chunxiao Liu, Zhengyu Zha, Chenhao Zhou, Yeh Chen, Weiya Xia, Ying-Nai Wang, Heng-Huan Lee, Yirui Yin, Meisi Yan, Chiung-Wen Chang, Li-Chuan Chan, Yufan Qiu, Hui Li, Chia-Wei Li, Jung-Mao Hsu, Jennifer L. Hsu, Shao-Chun Wang, Ning Ren, Mien-Chie Hung
Summary: This study demonstrates that RNase7 acts as a high-affinity ligand for ROS1 in hepatocellular carcinoma, with important pathological and therapeutic implications. High levels of ROS1 and RNase7 expression are strongly associated with poor prognosis in HCC patients, suggesting potential benefits of anti-ROS1 treatment for these individuals.
JOURNAL OF HEPATOLOGY
(2021)
Article
Pediatrics
Niels Lodeweyckx, Kristien Wouters, Kristien J. Ledeganck, Dominique Trouet
Summary: Children with nephrotic or proteinuric glomerular diseases show a lower uEGF/uCreat, indicating early tubulo-interstitial damage, which is reversible under ACE inhibition except in minimal change disease. The response to ACE inhibition differs among different glomerular diseases. Additionally, patients with genetic predisposition display lower uEGF/uCreat levels.
FRONTIERS IN PEDIATRICS
(2021)
Article
Oncology
Junjie Ao, Tetsuhiro Chiba, Hiroaki Kanzaki, Kengo Kanayama, Shuhei Shibata, Akane Kurosugi, Terunao Iwanaga, Motoyasu Kan, Takafumi Sakuma, Na Qiang, Yaojia Ma, Ryuta Kojima, Yuko Kusakabe, Masato Nakamura, Kazufumi Kobayashi, Soichiro Kiyono, Naoya Kanogawa, Tomoko Saito, Ryo Nakagawa, Takayuki Kondo, Sadahisa Ogasawara, Eiichiro Suzuki, Shingo Nakamoto, Ryosuke Muroyama, Akinobu Tawada, Jun Kato, Tatsuo Kanda, Hitoshi Maruyama, Naoya Kato
Summary: The study aimed to investigate the potential of Ang2 as a diagnostic and prognostic biomarker in HCC, finding its serum levels weakly correlated with AFP and DCP, with high specificity and comparability, and potential diagnostic value in patients with normal AFP and DCP levels. Additionally, high serum Ang2 levels were associated with poor prognosis and shorter recurrence-free survival in HCC patients.
Article
Oncology
Karel Pacak, Mark Kidd, Leah Meuter, Irvin M. Modlin
Summary: The study demonstrated the high accuracy of NET-specific gene blood analysis in diagnosing pheochromocytomas and paragangliomas. The method also showed the ability to distinguish different tumor types and had good correlation with imaging tests. The results in pediatric patients were similar to adults, suggesting potential clinical utility of circulating NET transcript analysis in managing these patients.
ENDOCRINE-RELATED CANCER
(2021)
Article
Dermatology
Mirjana Ziemer, Viviane Fries, Maren Paulmann, Maja Mockenhaupt
Summary: This study analyzes data from the German Center for the Documentation of Severe Skin Reactions and identifies an increasing number of severe skin reactions associated with new immuno-oncologic drugs, kinase inhibitors, and biologics. The study confirms cases of Stevens-Johnson syndrome and toxic epidermal necrolysis caused by these drugs and also highlights other causative agents. Additionally, the study identifies skin reactions similar to epidermal necrolysis. Correct diagnosis is crucial for prognosis and treatment decisions.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2022)
Article
Multidisciplinary Sciences
Can -Tong Liu, Fang-Cai Wu, Yi-Xuan Zhuang, Xin-Yi Huang, Xin-Hao Li, Qi-Qi Qu, Yu-Hui Peng, Yi-Wei Xu, Shu-Lin Chen, Xu-Chun Huang
Summary: This study suggests that serum IGFBP7 may serve as a potential early diagnostic marker for gastric cancers.
Review
Dermatology
Sun Hye Shin, Young Gue Koh, Woo Geon Lee, Joon Seok, Kui Young Park
Summary: Epidermal growth factor (EGF) is essential for wound healing and tissue homeostasis. Recombinant human EGF (rhEGF) has been proven to promote skin wound healing and has potential clinical applications in dermatological conditions beyond wound healing. However, there is a scarcity of studies on its use in these conditions. In this article, we searched PubMed/Medline and Google Scholar databases to summarize the effects of rhEGF in various skin problems.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Oncology
Anil Bagri, Leanne Berry, Bert Gunter, Mallika Singh, Ian Kasman, Lisa A. Damico, Hong Xiang, Maike Schmidt, Germaine Fuh, Beth Hollister, Oliver Rosen, Greg D. Plowman
CLINICAL CANCER RESEARCH
(2010)
Article
Oncology
MJ Brauer, GL Zhuang, M Schmidt, J Yao, XM Wu, JS Kaminker, SS Jurinka, G Kolumam, AS Chung, A Jubb, Z Modrusan, T Ozawa, CD James, H Phillips, B Haley, RNW Tam, AC Clermont, JH Cheng, SX Yang, SM Swain, D Chen, SJ Scherer, H Koeppen, RF Yeh, P Yue, JP Stephan, P Hegde, N Ferrara, M Singh, C Bais
CLINICAL CANCER RESEARCH
(2013)
Article
Cell Biology
Adrian M. Jubb, Susan M. Sa, Navneet Ratti, Laura A. Strickland, Maike Schmidt, Christopher A. Callahan, Hartmut Koeppen
Article
Medicine, Research & Experimental
Leisa Johnson, Mahrukh Huseni, Tanya Smyczek, Anthony Lima, Stacey Yeung, Jason H. Cheng, Rafael Molina, David Kan, Ann De Maziere, Judith Klumperman, Ian Kasman, Yin Zhang, Mark S. Dennis, Jeffrey Eastham-Anderson, Adrian M. Jubb, Olivia Hwang, Rupal Desai, Maike Schmidt, Michelle A. Nannini, Kai H. Barck, Richard A. D. Carano, William F. Forrest, Qinghua Song, Daniel S. Chen, Louie Naumovski, Mallika Singh, Weilan Ye, Priti S. Hegde
JOURNAL OF CLINICAL INVESTIGATION
(2013)
Article
Oncology
Adrian M. Jubb, Laura A. Strickland, Scot D. Liu, Judy Mak, Maike Schmidt, Hartmut Koeppen
JOURNAL OF PATHOLOGY
(2012)
Article
Oncology
Christy C. Ong, Sarah Gierke, Cameron Pitt, Meredith Sagolla, Christine K. Cheng, Wei Zhou, Adrian M. Jubb, Laura Strickland, Maike Schmidt, Sergio G. Duron, David A. Campbell, Wei Zheng, Seameen Dehdashti, Min Shen, Nora Yang, Mark L. Behnke, Wenwei Huang, John C. Mckew, Jonathan Chernoff, William F. Forrest, Peter M. Haverty, Suet-Feung Chin, Emad A. Rakha, Andrew R. Green, Ian O. Ellis, Carlos Caldas, Thomas O'Brien, Lori S. Friedman, Hartmut Koeppen, Joachim Rudolph, Klaus P. Hoeflich
BREAST CANCER RESEARCH
(2015)
Article
Developmental Biology
Maike Schmidt, Kim Paes, Ann De Maziere, Tanya Smyczek, Stacey Yang, Alane Gray, Dorothy French, Ian Kasman, Judith Klumperman, Dennis S. Rice, Weilan Ye
Article
Cell Biology
Megan G. Davey, I. Robert Paton, Yili Yin, Maike Schmidt, Fiona K. Bangs, David R. Morrice, Terence Gordon Smith, Paul Buxton, Despina Stamataki, Mikiko Tanaka, Andrea E. Muensterberg, James Briscoe, Cheryll Tickle, Dave W. Burt
GENES & DEVELOPMENT
(2006)
Article
Multidisciplinary Sciences
LH Parker, M Schmidt, SW Jin, AM Gray, D Beis, T Pham, G Frantz, S Palmieri, K Hillan, DYR Stainier, FJ de Sauvage, WL Ye
Article
Anatomy & Morphology
M Schmidt, M Patterson, E Farrell, A Münsterberg
DEVELOPMENTAL DYNAMICS
(2004)
Article
Developmental Biology
M Schmidt, M Tanaka, A Münsterberg
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)