Article
Microbiology
Jaclyn A. Cusumano, Kathryn E. Daffinee, Paul Ugalde-Silva, Wolfgang Peti, Michel Arthur, Charlene Desbonnet, Louis B. Rice, Kerry L. LaPlante, Monica Garcia-Solache
Summary: Ampicillin-ceftriaxone is an effective treatment for Enterococcus faecalis endocarditis. This study characterized the penicillin-binding protein (PBP) profiles of different E. faecalis strains and tested for synergy to optimize beta-lactam treatment options for E. faecalis infections. Increased expression of PBP4 and PBP2B was observed in a strain with elevated MICs, but PBP2B did not significantly contribute to the elevated beta-lactam MICs. The combination of meropenem plus ceftriaxone or ertapenem plus ceftriaxone showed the most consistent synergistic activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Elise T. T. Zeiser, Scott A. A. Becka, John J. J. LiPuma, Krisztina M. M. Papp-Wallace
Summary: In this study, a new compound, ETX0462, was evaluated for its activity against multidrug-resistant Burkholderia cepacia complex and Burkholderia gladioli, with the results showing that ETX0462 demonstrated superior in vitro activity compared to currently available treatment options.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemical Research Methods
Thomas Krause, Behnam Keshavarzi, Jannes Dressel, Sascha Heitkam, Marion B. Ansorge-Schumacher
Summary: Currently, there is a major bottleneck in the availability of methods for downstream processing of enzymes. A study suggests that using surface-active proteins as fusion tags might be the key to establishing foam fractionation as a general method for enzyme downstream processing.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Peng Wang, Chen Shen, Qinqin Cong, Kaili Xu, Jialin Lu
Summary: The study identified and characterized a beta-lactamase from Ochrobactrum tritici involved in the biodegradation of penicillin V. The enzyme exhibited high activity against penicillin V and is expected to be used for future degradation of this antibiotic.
MICROBIAL CELL FACTORIES
(2021)
Review
Infectious Diseases
Davide Carcione, Claudia Siracusa, Adela Sulejmani, Valerio Leoni, Jari Intra
Summary: Beta-lactams are important antibiotics, but increasing resistance, especially among Gram-negative bacteria, poses a major threat to public health. Combining beta-lactam antibiotics with beta-lactamase inhibitors can help overcome resistance, but some beta-lactamases are resistant to existing inhibitors, leading to a need for new and more efficient inhibitors.
Article
Chemistry, Multidisciplinary
Masafumi Minoshima, Taro Umeno, Kohei Kadooka, Margaux Roux, Namiko Yamada, Kazuya Kikuchi
Summary: To understand protein function in live cells, it is important to monitor protein dynamics and sense their environment in real-time. We developed a versatile chemical protein-labeling tool that efficiently forms stable carbamoylated complexes with beta-lactamase, allowing long-term visualization of labeled proteins in live cells. The use of an alpha-fluorinated carboxylate ester-based prodrug enables cell membrane permeability and stable labeling of intracellular proteins. Combining this labeling tool with a pH-activatable fluorescent probe enables visual monitoring of lysosomal protein translocation during autophagy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Microbiology
Alaa R. M. Sayed, Nirav R. Shah, Kari B. Basso, Manasi Kamat, Yuanyuan Jiao, Bartolome Moya, Dhruvitkumar S. Sutaria, Yinzhi Lang, Xun Tao, Weiguo Liu, Eunjeong Shin, Jieqiang Zhou, Carolin Werkman, Arnold Louie, George L. Drusano, Jurgen B. Bulitta
Summary: There is no standard regimen for the treatment of M. abscessus infections, and the multitude of combinations that have been used clinically have had low success rates and high rates of toxicities. However, research has found that combination therapy with beta-lactam antibiotics may improve treatment outcomes and minimize toxicity in M. abscessus infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Biochemistry & Molecular Biology
Juan F. Martin, Ruben Alvarez-Alvarez, Paloma Liras
Summary: The widespread resistance to antibiotics is a serious problem in human society. It is proposed that antibiotic resistance genes in pathogenic bacteria may have originated from antibiotic-producing microorganisms. The model actinobacteria Streptomyces clavuligerus plays an important role in fighting against competing microorganisms through the synergistic effect between its produced cephamycin and secreted clavulanic acid. The high levels of resistance to cephamycin/cephalosporin in actinobacteria may be due to the presence of genes encoding penicillin-binding proteins (PBPs) in their beta-lactam clusters. The study suggests that these gene clusters have been moved between different actinobacteria through horizontal gene transfer. This article focuses on exploring the connection between beta-lactam biosynthesis and beta-lactam resistance mechanisms in producer actinobacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Minghui Zhang, Laixing Zhang, Runyu Guo, Chun Xiao, Jian Yin, Sensen Zhang, Maojun Yang
Summary: In this study, the cryoelectron microscopy structures of the human LACTB (hLACTB) were determined. It was found that the assembly of hLACTB filaments requires three interfaces and the formation of higher order helical structures facilitates its cleavage activity. Furthermore, the study showed that the middle region is necessary for substrate hydrolysis but not for filament formation. The inhibitor-bound structure suggests that hLACTB may cleave peptide bonds adjacent to aspartic acid residues.
Article
Infectious Diseases
Kamonwan Lunha, Wiyada Chumpol, Surasak Jiemsup, Sukuma Samngamnim, Pornchalit Assavacheep, Suganya Yongkiettrakul
Summary: The study aims to investigate the genetic basis for the decrease in susceptibility to penicillin and other beta-lactams among Streptococcus suis. The findings suggest a growing trend of reduced susceptibility to beta-lactams and the presence of substitutions within penicillin-binding proteins (PBPs) associated with this decrease. The study highlights the importance of genetic mutations in mediating antibiotic resistance in S. suis.
Article
Microbiology
Marta Toth, Mijoon Lee, Nichole K. Stewart, Sergei B. Vakulenko
Summary: Resistance to beta-lactams is a major problem in treating bacterial infections. In the case of Acinetobacter baumannii, resistance to beta-lactam antibiotics has resulted in the spread of multidrug-resistant isolates. This study found that PBP3 is essential for the survival of A. baumannii, and inhibiting PBP1a and PBP2, or PBPs in combination with LdtJ, could be potential strategies for designing new drugs against this pathogen.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Shannon G. Murphy, Andrew N. Murtha, Ziyi Zhao, Laura Alvarez, Peter Diebold, Jung-Ho Shin, Michael S. VanNieuwenhze, Felipe Cava, Tobias Doerr
Summary: In Vibrio cholerae, endopeptidases are essential for proper cell elongation and division, while aPBPs become crucial for maintaining structural integrity during endopeptidase insufficiency, and the Rod system remains active but contributes minimally to cell expansion under these conditions.
Review
Immunology
Dingle Yu, Danchun Guo, Yuejie Zheng, Yonghong Yang
Summary: With the widespread use of antibiotics, antimicrobial resistance (AMR) has become a global problem that endangers public health. The gene encoding penicillin binding protein 2X (pbp2x) has been found to be associated with group A Streptococcus (GAS) with reduced beta-lactam susceptibility.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Tsubasa Ikeda, Rihito Suzuki, Wanchun Jin, Jun-Ichi Wachino, Yoshichika Arakawa, Kouji Kimura
Summary: The first isolation of Streptococcus pyogenes with PBP2X substitution causing reduced in vitro beta-lactam susceptibility in Asia was reported. Despite beta-ICs being below the susceptible breakpoint of the CLSI, the strain still falls into the suspicious range.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Carmina Micelli, Yunfei Dai, Nicole Raustad, Ralph R. Isberg, Christopher G. Dowson, Adrian J. Lloyd, Edward Geisinger, Allister Crow, David I. Roper
Summary: Acinetobacter baumannii is a gram-negative bacterial pathogen that causes challenging nosocomial infections. The study reveals the molecular details of cell wall elongation inhibition upon zinc starvation in A. baumannii. The findings offer a perspective for dual inhibition of zinc-dependent peptidoglycan synthases and metallo-beta-lactamases by metal-chelating agents.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Medicinal
Jeffrey W. Johannes, Christopher R. Denz, Nancy Su, Allan Wu, Anna C. Impastato, Scott Mlynarski, Jeffrey G. Varnes, D. Bryan Prince, Justin Cidado, Ning Gao, Malcolm Haddrick, Natalie H. Jones, Shaobin Li, Xiuwei Li, Yang Liu, Toan B. Nguyen, Nichole O'Connell, Emma Rivers, Daniel W. Robbins, Ronald Tomlinson, Tieguang Yao, Xiahui Zhu, Andrew D. Ferguson, Michelle L. Lamb, John I. Manchester, Sylvie Guichard
Article
Chemistry, Medicinal
Adam B. Shapiro, Janelle Comita-Prevoir, Mark Sylvester
ACS INFECTIOUS DISEASES
(2019)
Article
Neurosciences
Deborah Huyghe, Andrew R. Denninger, Caroline M. Voss, Pernille Frank, Ning Gao, Nicholas Brandon, Helle S. Waagepetersen, Andrew D. Ferguson, Menelas Pangalos, Peter Doig, Stephen J. Moss
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Jon A. Read, Jonathan Tart, Philip B. Rawlins, Clare Gregson, Karen Jones, Ning Gao, Xiahui Zhu, Ron Tomlinson, Erin Code, Tony Cheung, Huawei Chen, Sameer P. Kawatkar, Andy Bloecher, Sharan Bagal, Daniel H. O'Donovan, James Robinson
ACS CHEMICAL BIOLOGY
(2019)
Article
Biochemical Research Methods
Jakub Luptak, Michal Bista, David Fisher, Liz Flavell, Ning Gao, Kate Wickson, Steven L. Kazmirski, Tina Howard, Philip B. Rawlins, David Hargreaves
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2019)
Article
Chemistry, Medicinal
Alita A. Miller, Adam B. Shapiro, Sarah M. McLeod, Nicole M. Carter, Samir H. Moussa, Ruben Tommasi, John P. Mueller
ACS INFECTIOUS DISEASES
(2020)
Article
Chemistry, Medicinal
Thomas F. Durand-Reville, Janelle Comita-Prevoir, Jing Zhang, Xiaoyun Wu, Tricia L. May-Dracka, Jan Antoinette C. Romero, Frank Wu, April Chen, Adam B. Shapiro, Nicole M. Carter, Sarah M. McLeod, Robert A. Giacobbe, Jeroen C. Verheijen, Sushmita D. Lahiri, Michael D. Sacco, Yu Chen, John P. O'Donnell, Alita A. Miller, John P. Mueller, Ruben A. Tommasi
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Microbiology
Krisztina M. Papp-Wallace, Adam B. Shapiro, Scott A. Becka, Elise T. Zeiser, John J. LiPuma, Douglas J. Lane, Rekha G. Panchal, John P. Mueller, John P. O'Donnell, Alita A. Miller
Summary: Burkholderia genus, including pathogens causing Melioidosis and Glanders, are hard to treat, but the combination of sulbactam-durlobactam (SUL-DUR) shows potential for treating these infections by inhibiting bacterial growth and reducing beta-lactamase activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Chemistry, Medicinal
Adam B. Shapiro, Ning Gao
Summary: ETX0282, an orally bioavailable prodrug of the diazabicyclooctane serine beta-lactamase inhibitor ETX1317, is currently being tested in combination with cefpodoxime proxetil for complicated urinary tract infections. ETX1317 contains a fluoroacetate moiety esterified with an isopropyl group in ETX0282 to provide high oral bioavailability. Studies on the interaction of ETX1317 with various beta-lactamases show that it has intermediate potency compared to other inhibitors like durlobactam and avibactam.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Medicinal
Adam B. Shapiro, Samir H. Moussa, Nicole M. Carter, Ning Gao, Alita A. Miller
Summary: Mutations in KPC-2 and KPC-3 beta-lactamase can confer resistance to ceftazidime-avibactam, but do not affect the ability of ETX1317 to overcome cefpodoxime resistance induced by KPC-3. This suggests that ETX1317 has potential in treating KPC-3 resistance.
ACS INFECTIOUS DISEASES
(2021)
Article
Microbiology
Elizabeth T. Montano, Jason F. Nideffer, Joseph Sugie, Eray Enustun, Adam B. Shapiro, Hannah Tsunemoto, Alan Derman, Kit Pogliano, Joe Pogliano
Summary: This study utilized bacterial cytological profiling to investigate the activity of compounds that may inhibit multiple cellular pathways, revealing molecules with specific activity against E. coli thymidylate kinase despite structural similarity to related compounds. Genetic analysis of resistant mutants identified thymidylate kinase as an intracellular target of some rhodanine-containing antibiotics. The study highlights the potential of bacterial cytological profiling in directing antibiotic discovery efforts and identifying molecules with specific in vivo activity.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Multidisciplinary Sciences
Thomas F. Durand-Reville, Alita A. Miller, John P. O'Donnell, Xiaoyun Wu, Mark A. Sylvester, Satenig Guler, Ramkumar Iyer, Adam B. Shapiro, Nicole M. Carter, Camilo Velez-Vega, Samir H. Moussa, Sarah M. McLeod, April Chen, Angela M. Tanudra, Jing Zhang, Janelle Comita-Prevoir, Jan A. Romero, Hoan Huynh, Andrew D. Ferguson, Peter S. Horanyi, Stephen J. Mayclin, Henry S. Heine, George L. Drusano, Jason E. Cummings, Richard A. Slayden, Ruben A. Tommasi
Summary: The study presents a rational design strategy for developing new antibiotics to combat infections caused by drug-resistant Gram-negative pathogens. The inhibitors are able to overcome multiple mechanisms of resistance and show potential clinical efficacy against superbugs.
Article
Microbiology
Samir H. Moussa, Adam B. Shapiro, Sarah M. Mcleod, Ramkumar Iyer, Nicole M. Carter, Yu-Kuo Tsai, L. Kristopher Siu, Alita A. Miller
Summary: Acinetobacter baumannii-calcoaceticus complex (ABC) causes severe infections that are difficult to treat, but sulbactam-durlobactam is an effective antibiotic for treating these infections. A global surveillance study showed that a small percentage of ABC isolates have developed resistance to this antibiotic, with metallo-beta-lactamases or penicillin-binding protein 3 mutations being the main driving factors.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Jessie Hao-Ru Hsu, Timothy Rasmusson, James Robinson, Fiona Pachl, Jon Read, Sameer Kawatkar, Daniel H. O'Donovan, Sharan Bagal, Erin Code, Philip Rawlins, Argyrides Argyrou, Ronald Tomlinson, Ning Gao, Xiahui Zhu, Elisabetta Chiarparin, Kelly Jacques, Minhui Shen, Haley Woods, Emma Bednarski, David M. Wilson, Lisa Drew, M. Paola Castaldi, Stephen Fawell, Andrew Bloecher
CELL CHEMICAL BIOLOGY
(2020)
Article
Microbiology
Sarah M. McLeod, Adam B. Shapiro, Samir H. Moussa, Michele Johnstone, Robert E. McLaughlin, Boudewijn L. M. de Jonge, Alita A. Miller
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
