Article
Immunology
Myriam Fernandez-Sendin, Claudia Augusta Di Trani, Angela Bella, Marcos Vasquez, Nuria Ardaiz, Celia Gomar, Leire Arrizabalaga, Sergio Ciordia, Fernando J. Corrales, Fernando Aranda, Pedro Berraondo
Summary: Apolipoprotein A-I mimetic peptide L37pA modulates the gene expression program of interferon alpha, reducing inflammatory pathways activation and inducing an anti-inflammatory effect in the liver. This modulation allows for the silencing of interferon alpha expression mediated by an adeno-associated virus, preventing lethal hematological toxicity in mice. The proteomic analysis confirms the impact of L37pA on several inflammatory pathways and indicates the activation of LXR/RXR and PPPAR alpha/gamma nuclear receptors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Wenmin Yuan, Kelsey Ernst, Rui Kuai, Emily E. Morin, Minzhi Yu, Denis O. Sviridov, Jie Tang, Ling Mei, Dan Li, Rose Ackermann, Alan T. Remaley, Anna Schwendeman
Summary: Synthetic high-density lipoproteins nanomedicine (sHDL) composed of ApoA-I mimetic peptides and lipids have shown promising results for the treatment of cardiovascular diseases. However, the understanding of how ApoA-I mimetic peptides affect the properties of sHDL and their relationship with potency is limited. This study evaluated different ApoA-I mimetic peptides in terms of their physical/chemical properties, cholesterol efflux, cholesterol esterification, remodeling, and pharmacokinetics/pharmacodynamics, highlighting the importance of considering overall performance for designing and optimizing ApoA-I mimetic peptides.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Article
Medicine, Research & Experimental
Laura Giorgi, Akseli Niemela, Esa-Pekka Kumpula, Ossi Natri, Petteri Parkkila, Juha T. Huiskonen, Artturi Koivuniemi
Summary: This study combines coarse-grained molecular dynamics simulations with complementary experiments to gain mechanistic insight into how apoA-I mimetic peptide 22A and its variants tune LCAT activity in peptide-lipid nanodiscs. Results show that peptide 22A forms transient antiparallel dimers at the rim of nanodiscs, and the removal of C-terminal lysine K22 decreases dimerization tendency and LCAT activity. Furthermore, simulations reveal that LCAT localizes preferentially to the rim of nanodiscs in a manner that shields important domains from water phase, and the conformation of LCAT in the rim affects its activity.
MOLECULAR PHARMACEUTICS
(2022)
Review
Cell Biology
Anna Wolska, Mart Reimund, Denis O. Sviridov, Marcelo J. Amar, Alan T. Remaley
Summary: Since the breakthrough of using insulin to treat diabetic patients in the 1920s, there has been a growing interest in developing various proteins and their peptide mimetics for treating medical disorders. Currently, there are over 60 approved peptides for human use and more than 150 peptides in clinical development. This review mainly focuses on peptide mimetics related to cardiovascular diseases, discussing the biochemistry, design, and clinical trials of peptides based on apoA-I, apoE, and apoC-II, while also addressing the limitations and challenges in developing apolipoprotein mimetic peptides into new drugs.
Article
Chemistry, Multidisciplinary
Michelle W. Lee, Elizabeth Wei-Chia Luo, Carlos Silvestre-Roig, Yashes Srinivasan, Kiyotaka Akabori, Patricia Lemnitzer, Nathan W. Schmidt, Ghee Hwee Lai, Christian D. Santangelo, Oliver Soehnlein, Gerard C. L. Wong
Summary: The study demonstrates the ability to create peptides or proteins that inhibit histone H4 membrane permeation, reducing cell death and inflammation. Experiments show that apoMP(1) can dose-dependently alleviate H4 cytotoxicity. Furthermore, administration of apoMP(1) is found to strongly prevent organ tissue damage.
Review
Cell Biology
Malgorzata Wygrecka, Ioannis Alexopoulos, Daniel P. Potaczek, Liliana Schaefer
Summary: ApoA-I plays an important role in reverse cholesterol transport and also possesses anti-inflammatory and antioxidative functions. Dysfunctional apoA-I or its low abundance can lead to the formation of foam cells in alveolar macrophages. Increased numbers of foam cells have been observed in the lungs of mice exposed to certain substances and in patients with lung fibrosis. Understanding the role of apoA-I in lung fibrosis provides potential avenues for therapeutic development.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lipika Mirdha, Tanusree Sengupta, Hirak Chakraborty
Summary: This study reveals that the signal peptide of proteins has a preference for the lipid composition of membranes during protein trafficking, with cholesterol playing a key role. This property can be utilized for the development of organelle-specific cargo delivery.
BIOPHYSICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Michael J. Haas, Shrina Parekh, Poonam Kalidas, Angela Richter, Firas Warda, Norman C. W. Wong, Masaaki Tokuda, Arshag D. Mooradian
Summary: This study demonstrates that rare sugars D-allulose and D-tagatose can increase the production of apo A-I by enhancing the binding of transcription factor SP1 to the promoter. These sugars modulate insulin responsive genes and may have anti-atherogenic properties.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Naoya Kato, Sakura Yamada, Rino Suzuki, Yoshiki Iida, Makoto Matsumoto, Shintaro Fumoto, Hidetoshi Arima, Hidefumi Mukai, Shigeru Kawakami
Summary: This study screened and compared the functionality of eight BBB-penetrating peptides, and found that ApoEdp exhibited the best brain-targeting and BBB permeability. ApoEdp-SG-lipid was newly synthesized and ApoEdp-modified PEGylated liposomes were prepared. The results demonstrated that ApoEdp-modified PEGylated liposomes had the ability to cross the BBB and target the brain.
Article
Pharmacology & Pharmacy
Hongfang Jiang, Xue Bai
Summary: This study suggests that ApoAI MP may have the potential to improve the behavioral performance of PD mice. By enhancing antioxidant and anti-inflammatory capacities, ApoAI MP can alleviate symptoms in PD mice.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Herman E. Popeijus, Willem Zwaan, Jehad Z. Tayyeb, Jogchum Plat
Summary: ApoA-I plays a crucial role in HDL metabolism and RCT. Lower serum ApoA-I concentrations during inflammation may lead to dysfunctional HDL particles. Modulating hepatic lipoprotein metabolism with compounds like SCFAs shows promise in increasing ApoA-I concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Da-Sheng Ning, Jian Ma, Yue-Ming Peng, Yan Li, Ya-Ting Chen, Shang-Xuan Li, Zui Liu, Yu-Quan Li, Yi-Xin Zhang, Yu-Peng Jian, Zhi-Jun Ou, Jing-Song Ou
Summary: The study revealed that hypercholesterolemia increases uncoupled eNOS activity by reducing GCH-1 concentration, leading to lower BH4 levels. D-4F reduces uncoupled eNOS activity by increasing BH4 levels through GCH-1 expression and by decreasing eNOS phosphorylation and eNOS-HSP90 association.
Article
Cell Biology
Sam Vanherle, Winde Jorissen, Tess Dierckx, Melanie Loix, Elien Grajchen, Fleur Mingneau, Jeroen Guns, Pascal Gervois, Ivo Lambrichts, Jonas Dehairs, Johannes Swinnen, Monique T. Mulder, Alan T. Remaley, Mansour Haidar, Jerome J. A. Hendriks, Jeroen J. F. Bogie
Summary: The inability of the central nervous system to induce proper remyelination is a key feature of demyelinating diseases. Dysregulated innate immune response and accumulation of myelin-derived lipids contribute to the failure of remyelination. The molecule ApoA-I mimetic peptide 5A enhances remyelination by promoting clearance and metabolism of myelin debris through the CD36 protein, regulated by the ABCA1-JAK2-STAT3 signaling pathway.
Review
Biochemistry & Molecular Biology
Eric J. J. Niesor, Elie Nader, Anne Perez, Francois Lamour, Renee Benghozi, Alan Remaley, Swee Lay Thein, Philippe Connes
Summary: Cell membrane lipid composition, especially cholesterol, affects the functions of enzymes, transporters, and receptors in red blood cells. High cholesterol content in the membrane can impact the transport and delivery of oxygen and carbon dioxide, and lead to physiological and pathological consequences in peripheral tissues. The potential therapeutic role of high-density lipoproteins (HDL) in removing excess cholesterol from red blood cell membranes has not been well studied. This study examines the potential role of HDL in regulating cholesterol levels in red blood cells, especially in sickle cell disease.
Article
Biochemistry & Molecular Biology
Ifat Alsharif, Loubna Boukhzar, Benjamin Lefranc, David Godefroy, Juliette Aury-Landas, Jean-Luc do Rego, Jean-Claude do Rego, Frederic Naudet, Arnaud Arabo, Abdeslam Chagraoui, David Maltete, Abdelhamid Benazzouz, Catherine Bauge, Jerome Leprince, Abdel G. Elkahloun, Lee E. Eiden, Youssef Anouar
Summary: PSELT, derived from SELENOT, shows promise as a therapeutic candidate for Parkinson's disease by targeting oxidative stress at multiple intracellular levels.
Meeting Abstract
Urology & Nephrology
A. Burnett, F. El Rassi, D. Darbari, J. Paulose, D. Laine, D. Purkayastha, G. Kato
JOURNAL OF SEXUAL MEDICINE
(2020)
Review
Immunology
Oluwabukola T. Gbotosho, Maria G. Kapetanaki, Gregory J. Kato
Summary: Hemolysis is a common feature in various diseases, resulting in the release of harmful metabolites from hemoglobin and exceeding the capacity of protective mechanisms. In Sickle Cell Disease (SCD), circulating heme plays a significant role in disease progression by promoting oxidative stress and proinflammatory responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Letter
Hematology
Annelies J. van Vuren, Caterina P. Minniti, Laurel Mendelsohn, John H. Baird, Gregory J. Kato, Eduard J. van Beers
AMERICAN JOURNAL OF HEMATOLOGY
(2021)
Article
Medicine, General & Internal
James F. Casella, Bruce A. Barton, Julie Kanter, L. Vandy Black, Suvankar Majumdar, Adlette Inati, Yasser Wali, Richard A. Drachtman, Miguel R. Abboud, Yurdanur Kilinc, Beng R. Fuh, Murtadha K. Al-Khabori, Clifford M. Takemoto, Emad Salman, Sharada A. Sarnaik, Nirmish Shah, Claudia R. Morris, Jennifer Keates-Baleeiro, Ashok Raj, Ofelia A. Alvarez, Lewis L. Hsu, Alexis A. Thompson, India Y. Sisler, Betty S. Pace, Suzie A. Noronha, Joseph L. Lasky, Elena Cela de Julian, Kamar Godder, Courtney Dawn Thornburg, Natalie L. Kamberos, Rachelle Nuss, Anne M. Marsh, William C. Owen, Anne Schaefer, Cameron K. Tebbi, Christophe F. Chantrain, Debra E. Cohen, Zeynep Karakas, Connie M. Piccone, Alex George, Jason M. Fixler, Tammuella C. Singleton, Thomas Moulton, Charles T. Quinn, Clarisse Lopes de Castro Lobo, Abdulkareem M. Almomen, Meenakshi Goyal-Khemka, Philip Maes, Marty Emanuele, Rebecca T. Gorney, Claire S. Padgett, Ed Parsley, Shari S. Kronsberg, Gregory J. Kato, Mark T. Gladwin
Summary: The study found that poloxamer 188 did not significantly shorten the duration of vaso-occlusive episodes in children and adults with sickle cell disease. Although there were some differences observed in participants under 16 years old, the overall effect was not significant.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2021)
Review
Biochemistry & Molecular Biology
Himanshu Garg, Kristina J. Tatiossian, Karsten Peppel, Gregory J. Kato, Eva Herzog
Summary: SCD is a common monogenic disorder caused by a point mutation in the beta-globin gene, making it a prototypical target for precision therapy. Both the mutant beta-globin protein and its downstream pathophysiology are intensively researched pharmacological targets. Recent advances in HSC transplantation and gene therapy platforms have expanded potentially curative options for SCD patients.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Hematology
Solomon Johnson, Victor R. Gordeuk, Roberto Machado, J. Simon R. Gibbs, Mariana Hildesheim, Jane A. Little, Gregory J. Kato, Mark T. Gladwin, Mehdi Nouraie
Summary: This study evaluated the predictors and prognostic value of exercise-induced vital sign changes (VSC) during the six-minute walk test (6MWT) in adult sickle cell disease (SCD) patients. The findings showed that increases in heart rate and blood pressure, as well as oxygen desaturation, are common during the 6MWT for SCD patients. Additionally, an increase in systolic blood pressure (SBP) during the 6MWT was associated with improved survival, indicating potential benefits in stroke volume capacity for these patients.
AMERICAN JOURNAL OF HEMATOLOGY
(2021)
Meeting Abstract
Hematology
Varshini Babu, Jane A. Little, Claudia R. Morris, Roberto Machado, J. Simon R. Gibbs, Gregory J. Kato, Victor R. Gordeuk, Mark T. Gladwin, Yingze Zhang, Seyed Mehdi Nouraie
Article
Medicine, General & Internal
Thomas Gentinetta, John D. Belcher, Valerie Bruegger-Verdon, Jacqueline Adam, Tanja Ruthsatz, Joseph Bain, Daniel Schu, Lisa Ventrici, Monika Edler, Hadi Lioe, Kalpeshkumar Patel, Chunsheng Chen, Julia Nguyen, Fuad Abdulla, Ping Zhang, Andreas Wassmer, Meena Jain, Marcel Mischnik, Matthias Pelzing, Kirstee Martin, Roslyn Davis, Svetlana Didichenko, Alexander Schaub, Nathan Brinkman, Eva Herzog, Adrian Zuercher, Gregory M. Vercellotti, Gregory J. Kato, Gerald Hoebarth
Summary: People living with sickle cell disease (SCD) often suffer from acute pain due to blood vessel blockage. This study discovered that hemopexin, a protein found in plasma, can alleviate the symptoms of vaso-occlusion by inhibiting inflammatory reactions and reducing the expression of adhesion proteins. Animal experiments and clinical trials have shown promising results, suggesting that hemopexin could be a potential treatment for SCD.
JOURNAL OF CLINICAL MEDICINE
(2022)
Letter
Hematology
Anusha Vittal, Hawwa Alao, Julian Hercun, Bashar Sharma, Arsalan Khan, Disha Sharma, Wilson Lee, Devika Kapuria, Matthew Hsieh, John Tisdale, Courtney Fitzhugh, David Kleiner, Elliot Levy, Richard Chang, Anna Conrey, Elenita Rivera, Amy Huang, Gil Ben Yakov, Gregory J. Kato, Mark T. Gladwin, Swee Lay Thein, Christopher Koh, Theo Heller
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Review
Cell Biology
Tirthadipa Pradhan-Sundd, Gregory J. Kato, Enrico M. Novelli
Summary: Sickle cell disease is a common genetic disorder that can lead to liver dysfunction, with therapeutic options still not well understood. Animal models have played a crucial role in studying the molecular mechanisms and potential treatments for SCD-related liver diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mara Carreno, Maria F. Pires, Steven R. Woodcock, Tomasz Brzoska, Samit Ghosh, Sonia R. Salvatore, Fei Chang, Nicholas K. H. Khoo, Matthew Dunn, Nora Connors, Shuai Yuan, Adam C. Straub, Stacy G. Wendell, Gregory J. Kato, Bruce A. Freeman, Solomon F. Ofori-Acquah, Prithu Sundd, Francisco J. Schopfer, Dario A. Vitturi
Summary: The up-regulation of kynurenine metabolism leads to the production of immunomodulatory Kyn-CKA. In sickle cell disease, increased synthesis of Kyn-CKA inhibits inflammation and protects tissues from damage.
Meeting Abstract
Hematology
Svetlana Didichenko, Alexey Navdaev, Moritz Saxenhofer, Stefanie Graeter, Alexander Schaub, Alicia Rovo, Gregory J. Kato, Bronwyn Kingwell
Article
Genetics & Heredity
Mengna Zhang, William B. Hillegass, Xue Yu, Suvankar Majumdar, J. Daryl Pollard, Erin Jackson, Jarrod Knudson, Douglas Wolfe, Gregory J. Kato, Joseph F. Maher, Hao Mei
Summary: This study identified several candidate genetic variants associated with QTc interval prolongation in sickle cell disease (SCD) patients and found serum alanine transaminase (ALT) to be a modifying factor. The association of a CPS1 gene variant in both QTc and JTc duration suggests the involvement of the urea cycle and nitric oxide metabolism in cardiac repolarization in SCD. Larger replication studies are needed to confirm these findings and elucidate the underlying mechanisms.
Meeting Abstract
Hematology
Jacqueline Adam, Thomas Gentinetta, Svetlana Diditchenko, Alexander Schaub, Gregory J. Kato, Nathan Brinkman, Adrian Zuercher
Meeting Abstract
Biochemistry & Molecular Biology
Mara Carreno, Maria Pires, Steven Woodcock, Sonia Salvatore, Gregory Kato, Samit Ghosh, Solomon Ofori-Acquah, Bruce Freeman, Francisco Schopfer, Dario Vitturi
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)