Article
Gastroenterology & Hepatology
Mohammad Bashashati, Emma Leishman, Heather Bradshaw, Solmaz Sigaroodi, Eric Tatro, Tamis Bright, Richard McCallum, Irene Sarosiek
Summary: The study revealed that endocannabinoids and cannabimimetic fatty acid derivatives are reduced in female patients with diabetic gastroparesis, potentially contributing to symptom development in these subjects. No similar changes were observed in male patients.
NEUROGASTROENTEROLOGY AND MOTILITY
(2021)
Review
Psychiatry
S. Alisha Epps
Summary: Depression and epilepsy show bidirectional comorbidity, and exploring the endocannabinoid system may lead to more effective treatments for both conditions.
FRONTIERS IN PSYCHIATRY
(2022)
Article
Chemistry, Analytical
Ernesto de Jesus Zapata Flores, Nguyen Kim Ngan Bui, Sigrid Selberg, Koit Herodes, Ivo Leito
Summary: This study proposes derivatization reagents based on azobenzene, AzoB and AzoC, for LC-ESI-MS analysis of free amino acids in fermented beverages and juices. The results show that AzoB can be used for targeted and non-targeted analysis, while AzoC has advantages such as fast reaction and good reactivity with secondary amines but is affected by side products for quantitation.
Article
Biochemistry & Molecular Biology
Runjin Zhao, Biling Huang, Gang Lu, Songsen Fu, Jianxi Ying, Yufen Zhao
Summary: Amino acid (AA) analysis is crucial in various fields such as biochemistry, food science, and clinical medicine. However, due to limitations, AAs often require derivatization for better separation and determination. In this study, a liquid chromatography-mass spectrometry (LC-MS) method utilizing urea as a derivatization agent was introduced. The urea-derivatized products of 20 AAs exhibited enhanced separation and detection compared to the underivatized counterparts. This fast, simple, and cost-effective method showed potential for AA analysis in complex samples, as demonstrated using a cell culture media model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Stefania Sut, Mario Malagoli, Stefano Dall'Acqua
Summary: This study evaluated the influences of silicon foliar application on berry composition in four grapevine cultivars. Results showed that silicon induced cultivar specific changes in berry composition, such as significant increase in anthocyanins in Oseleta cultivar, increased proline levels in Garganega, and decreased tartaric acid in Chardonnay.
Article
Biochemical Research Methods
Feiyang Li, Ryan Karongo, Despoina Mavridou, Jeannie Horak, Adrian Sievers-Engler, Michael Laemmerhofer
Summary: Enantioselective amino acid analysis is an important technique in pharmaceutical, biomedical, and food sciences. However, simultaneously analyzing all chiral proteinogenic amino acids using a single method with one column and one condition is still challenging. In this study, an enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS) assay using Chiralpak QN-AX as a chiral column was developed, enabling efficient separation of D- and L-amino acids.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Plant Sciences
Dhiraj Dokwal, Jean-Christophe Cocuron, Ana Paula Alonso, Rebecca Dickstein
Summary: Medicago truncatula undergoes metabolic changes in response to phosphorus deprivation, with a decrease in organic acids and phosphorylated metabolites in leaves and an increase in sugars and amino acids in the whole plant.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Chemistry, Applied
Lei Ye, Bo Zhang, Jie Zhou, Xiaoping Zhang, Wei Tan, Xiaolin Li
Summary: This study investigates the metabolic profiles of Auricularia cornea grown on pinecone substrates, revealing a rich variety of amino acid metabolites and significant differences related to protein synthesis. The findings contribute valuable insights into the nutritional and medicinal properties of A. cornea grown on a pinecone substrate.
Article
Food Science & Technology
Nanyu Li, Lanlan Jiang, Yiyi Liu, Shimei Zou, Min Lu, Huaming An
Summary: In this study, the metabolomics of Rosa roxburghii fruit at three developmental stages were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 533 metabolites were identified, with 339 significantly altered. The correlation between total phenols, flavonoids, amino acids and in vitro antioxidant activity was determined. Furthermore, the co-enrichment analysis of metabolome and transcriptome focused on amino acid, phenylpropanoid, and flavonoid biosynthesis pathways. The networks of amino acid, phenolic acid, and flavonol biosynthesis were constructed, and the gene expression was detected in different R. roxburghii genotypes. Key metabolites were identified in the in vitro antioxidant activities at different developmental stages and in different genotypes of R. roxburghii. This study provides novel insights into the synthesis of flavonoids, polyphenols, and amino acids in R. roxburghii.
Article
Biochemical Research Methods
Alexander Reiter, Laura Herbst, Wolfgang Wiechert, Marco Oldiges
Summary: The utilization of small-scale, highly parallelized cultivation platforms embedded in laboratory robotics accelerates microbial phenotyping and bioprocess development, generating a bottleneck in bioanalytical bioprocess sample analytics. This study developed and evaluated six amino acid quantification methods, confirming the versatility for metabolite profiling studies of microbial cultivation supernatants, with dilute and shoot methods showing higher throughput and comparable performance.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Neurosciences
Carol A. Gianessi, Stephanie M. Groman, Jane R. Taylor
Summary: Emerging data suggest that endocannabinoid signaling is crucial for habitual behavior formation, and inhibiting FAAH and MAGL disrupts habit formation while AM251 exposure affects response rates. Further studies are needed to clarify the role of CB1R signaling in habitual responding and the potential clinical utility of augmenting endocannabinoids.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2022)
Article
Chemistry, Analytical
Federico Fanti, Flaminia Vincenti, Giulia Imparato, Camilla Montesano, Lucia Scipioni, Francesca Ciaramellano, Daniel Tortolani, Sergio Oddi, Mauro Maccarrone, Dario Compagnone, Manuel Sergi
Summary: The present study developed a fast and reliable method to analyze endocannabinoids and their conjugated congeners in brain tissue. The method involved homogenizing the samples and using a micro solid phase extraction procedure for clean-up. UHPLC-MS/MS was employed for the analysis, and polarity switching was applied during the run to detect conjugated forms. The method achieved low limits of quantification and demonstrated good extraction recoveries.
Article
Cell Biology
Anne-Sophie Archambault, Francesco Tinto, Elizabeth Dumais, Volatiana Rakotoarivelo, Magdalena Kostrzewa, Pier-Luc Plante, Cyril Martin, Melissa Simard, Cristoforo Silvestri, Roxane Pouliot, Michel Laviolette, Louis-Philippe Boulet, Rosa Maria Vitale, Alessia Ligresti, Vincenzo Di Marzo, Nicolas Flamand
Summary: Endocannabinoids 2-arachidonoyl-glycerol and N-arachidonoyl-ethanolamine are lipids that regulate various physiological processes, including inflammation, and are being studied as potential anti-inflammatory agents. Human eosinophils and neutrophils have been found to metabolize 1-linoleoyl-glycerol (1-LG) and N-linoleoyl-ethanolamine (LEA) into novel endogenous 15-lipoxygenase metabolites 13-HODE-G and 13-HODE-EA, although the biological significance of these metabolites is not yet fully understood.
Article
Biochemistry & Molecular Biology
Aradhana Kartikeyan, Vinduja Vasudevan, Aakash John Peter, Nagasathiya Krishnan, Devadasan Velmurugan, Palaniyandi Velusamy, Periasamy Anbu, Perumal Palani, Pachaiappan Raman
Summary: This study investigated the effects of different incubation periods on the contents of amino acids, proteins, glycosylated proteins, and metabolites in germinated and ungerminated mung bean seeds. The results showed that soaking the seeds for 6 hours resulted in the most successful germination. Furthermore, it was found that the seeds soaked for 6 hours and germinated for 24 hours had the highest number of glycosylated proteins. Additionally, the study revealed that the concentration of amino acids increased after 3, 6, and 9 hours of soaking. Thus, mung bean seeds soaked for 6 hours and germinated for 24 hours can be considered as a potential nutritional source.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agriculture, Multidisciplinary
Susanne I. Wudy, Verena K. Mittermeier-Klessinger, Andreas Dunkel, Karin Kleigrewe, Regina Ensenauer, Corinna Dawid, Thomas F. Hofmann
Summary: Amino acids and acylcarnitines are important biomarkers for the body's energy state and can be used to diagnose certain inborn errors of metabolism. This study developed a quantitative high-throughput method to analyze these compounds in serum samples, using minimal amounts of serum and isotopically labeled standards for quantification. The method was successfully validated and applied to serum samples from infants, showing excellent reproducibility and enabling simultaneous profiling of amino acids and acylcarnitines.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Matthew D. Bartolowits, Jonathon M. Gast, Ashlee J. Hasler, Anthony M. Cirrincione, Rachel J. O'Connor, Amr H. Mahmoud, Markus A. Lill, Vincent Jo Davisson
Article
Behavioral Sciences
Zhong Ding, Gregory T. Knipp, Richard M. van Rijn, Julia A. Chester, Val J. Watts
Summary: Heterologous sensitization of adenylyl cyclase is enhanced cAMP response following G alpha(i/o)-coupled receptors activation, which can be abolished by pharmacological inhibition of Cullin3 with MLN4924. MLN4924 was found to reduce alcohol-induced locomotor sensitization and allodynia in an inflammatory pain model, potentially through blocking AC sensitization. These findings suggest a new pathway for drug development for disorders associated with AC sensitization.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mee Jung Ko, Terrance Chiang, Arbaaz A. Mukadam, Grace E. Mulia, Anna M. Gutridge, Angel Lin, Julia A. Chester, Richard M. van Rijn
Summary: In mice experiments, signaling through beta-arrestin was found to suppress anxiety and fear-related behaviors, while signaling through G proteins increased fear-related behavior. The results indicate a complex role of G protein-coupled receptors (GPCRs) in regulating anxiety and fear emotions.
Article
Multidisciplinary Sciences
Jason R. Nielson, Anjali K. Nath, Kim P. Doane, Xu Shi, Jangwoen Lee, Emily G. Tippetts, Kusumika Saha, Jordan Morningstar, Kevin G. Hicks, Adriano Chan, Yanbin Zhao, Amy Kelly, Tara B. Hendry-Hofer, Alyssa Witeof, Patrick Y. Sips, Sari Mahon, Vikhyat S. Bebarta, Vincent Jo Davisson, Gerry R. Boss, Jared Rutter, Calum A. MacRae, Matthew Brenner, Robert E. Gerszten, Randall T. Peterson
Summary: Cyanide acts as a metabolic poison by inhibiting cytochrome c oxidase and other metabolic enzymes, causing metabolic perturbations. Glyoxylate has been identified as a potential cyanide countermeasure that can effectively and rapidly mitigate cyanide toxicity in animal models.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
Caroline B. Plescia, Aaron R. Lindstrom, Maritza Quintero, Patrick Keiser, Manu Anantpadma, Robert Davey, Robert Stahelin, V. Jo Davisson
Summary: The study reports the inhibition of Ebola virus cell entry by selectively inhibiting V-ATPase. The compounds show improved selectivity and minimal cytotoxicity.
ACS INFECTIOUS DISEASES
(2022)
Article
Medicine, General & Internal
Vallabh Suresh, Kaleb Byers, Ummadisetti Chinna Rajesh, Francesco Caiazza, Gina Zhu, Charles S. Craik, Kimberly Kirkwood, Vincent Jo Davisson, Daniel A. Sheik
Summary: The gastricsin activity assay developed in this study offers a rapid and accurate method for distinguishing between mucinous and non-mucinous pancreatic cyst fluids. The assay shows improvements in differentiation compared to standard ELISA assays and has a minimal impact on standard clinical care.
Article
Chemistry, Medicinal
Matthew M. Behymer, Huaping Mo, Naoaki Fujii, Vallabh Suresh, Adriano Chan, Jangweon Lee, Anjali K. Nath, Kusumika Saha, Sari B. Mahon, Matthew Brenner, Calum A. MacRae, Randall Peterson, Gerry R. Boss, Gregory T. Knipp, Vincent Jo Davisson
Summary: The use of platinum-based agents has shown promise in mitigating cyanide toxicity, both in vitro and in vivo. The addition of sulfur ligands enhances the cyanide scavenging properties of platinum and leads to robust and enhanced potency. These findings suggest a potential new lead for the development of next-generation cyanide countermeasures.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Toxicology
Vik S. Bebarta, Xu Shi, Shunning Zheng, Tara B. Hendry-Hofer, Carter C. Severance, Matthew M. Behymer, Gerry R. Boss, Sari Mahon, Matthew Brenner, Gregory T. Knipp, Vincent Jo Davisson, Randall T. Peterson, Calum A. MacRae, Jared Rutter, Robert E. Gerszten, Anjali K. Nath
Summary: The study investigated the efficacy of intramuscular glyoxylate in a preclinical swine model of lethal cyanide poisoning. The results demonstrated that intramuscular administration of glyoxylate improved survival and clinical outcomes, and had positive effects on the biochemical effects of cyanide.
TOXICOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Digambar Kumar Waiker, Akash Verma, Gajendra T. Akhilesh, T. A. Gajendra, Namrata Singh, Anima Roy, Hagera Dilnashin, Vinod Tiwari, Surendra Kumar P. Trigun, Surya P. Singh, Sairam Krishnamurthy, Prem Lama, Vincent Jo Davisson, Sushant Kumar Shrivastava
Summary: This study successfully designed and synthesized a new class of compounds for the treatment of Alzheimer's disease (AD) through a multitargeted directed ligand design approach. Compound 5d and 5f showed inhibitory activity against key enzymes involved in AD pathology and reduced the aggregation of Aβ. They also demonstrated neuroprotective effects and improved learning and memory behaviors in AD mouse models.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Food Science & Technology
Soyol Enkh-Amgalan, Josephine M. Brown-Leung, Tauqeerunnisa Syeda, Rachel M. Nolan, Jason R. Cannon, Julia A. Chester
Summary: This study investigated the effects of the PD-related neurotoxicant PQ on binge-like alcohol drinking and striatal monoamines in mice bred for high alcohol preference. It found that PQ decreased alcohol drinking and ventral striatal DOPAC levels in male mice but not in females.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Chemistry, Analytical
Vallabh Suresh, Daniel A. A. Sheik, Tyler C. C. Detomasi, Tianqi Zhao, Theresa Zepeda, Shyam Saladi, Ummadisetti Chinna Rajesh, Kaleb Byers, Charles S. S. Craik, Vincent Jo Davisson
Summary: With the shift of COVID-19 from pandemic to endemic, the focus of diagnostics will change from rapid detection to stratification for vulnerable patients. A fluorescent and Raman spectroscopic assay for SARS-CoV-2 3CL protease has been developed, which can be used sequentially with a fluorescent ACE2 activity measurement in the same sample. The prototype assay shows promising results in saliva, allowing non-invasive sampling and quick detection of ACE2 and 3CL protease activity.
Meeting Abstract
Substance Abuse
S. Enkh-Amgalan, J. M. Brown, T. B. Syeda, R. M. Foguth, J. R. Cannon, J. A. Chester
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2022)
Meeting Abstract
Substance Abuse
I. Mueller, S. Sangha, J. A. Chester
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2020)
Article
Chemistry, Multidisciplinary
Matthew D. Bartolowits, Meiguo Xin, Dino P. Petrov, Thomas J. Tague, Vincent Jo Davisson
Article
Biochemistry & Molecular Biology
Jordan Morningstar, Jangwoen Lee, Tara Hendry-Hofer, Alyssa Witeof, Tiffany Lyle, Gregg Knipp, Calum A. MacRae, Gerry R. Boss, Randall T. Peterson, Vincent J. Davisson, Robert E. Gerszten, Vikhyat S. Bebarta, Sari Mahon, Matt Brenner, Anjali K. Nath
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
