Article
Polymer Science
Boris Shaskolskiy, Ilya Kandinov, Dmitry Kravtsov, Alexandra Vinokurova, Sofya Gorshkova, Marina Filippova, Alexey Kubanov, Victoria Solomka, Dmitry Deryabin, Ekaterina Dementieva, Dmitry Gryadunov
Summary: A multiplex assay using a low-density hydrogel microarray is developed to identify genomic substitutions in N. gonorrhoeae determining resistance to treatment agents. The microarray accurately identifies drug resistance determinants and single-nucleotide substitutions, with results consistent with Sanger sequencing. Analysis of clinical isolates in Russia over 5 years demonstrates the utility of hydrogel microarrays in monitoring antibiotic resistance spread.
Article
Genetics & Heredity
Katarzyna Kowalczyk, Magdalena Bartnik-Glaska, Marta Smyk, Izabela Plaskota, Joanna Bernaciak, Marta Kedzior, Barbara Wisniowiecka-Kowalnik, Krystyna Jakubow-Durska, Natalia Braun-Walicka, Artur Barczyk, Maciej Geremek, Jennifer Castaneda, Anna Kutkowska-Kazmierczak, Pawel Wlasienko, Marzena Debska, Anna Kucinska-Chahwan, Tomasz Roszkowski, Szymon Kozlowski, Boyana Mikulska, Tadeusz Issat, Ewa Obersztyn, Beata Anna Nowakowska
Summary: Congenital heart defects are common in newborns, and array comparative genomic hybridization is recommended as the first-line test for prenatal diagnosis of fetal heart defects. This method can help identify pathogenic variants and genetic loci associated with heart defects.
Article
Engineering, Environmental
Vinicius Diniz, Susanne Rath
Summary: This study investigates the adsorption capacity of two activated carbons on five model contaminants in different aqueous matrices. The results show that the AC1 has a higher adsorption capacity in the downflow mode, but preferential pathways reduce its useful lifetime. Additionally, the wastewater treatment effluent and pretreated water have a significant impact on the adsorption capacity of the contaminants.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Immunology
Danielle Rodrigues Cortez, Fabio Mitsuo Lima, Joao Luis Reis-Cunha, Daniella Castanheira Bartholomeu, Rolando Andre Rios Villacis, Silvia Regina Rogatto, Andre Guilherme Costa-Martins, Fernanda Sycko Marchiano, Rafaela Andrade do Carmo, Jose Franco da Silveira, Marjorie Mendes Marini
Summary: In this study, the genetic differences between two lineages of Chagas disease-causing parasite T. cruzi were analyzed using intraspecific array-based comparative genomic hybridization (aCGH). The results showed that clone D11 had more chromosomal gains and losses compared to the parental G strain. These genomic changes suggest a dynamic genome in clone D11 that responds to environmental stress by altering gene copy numbers and generating segmental aneuploidy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Mehmet Sakir Ece, Sinan Kutluay
Summary: This study investigates the adsorption behavior of volatile organic compounds (VOCs) on cellulose-modified nanoparticles. The results show that the adsorption capacity of the VOCs is influenced by competition between different components, leading to a decrease in adsorption capacity.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Evolutionary Biology
Sungsik Kong, Laura S. Kubatko
Summary: Interspecific hybridization plays a vital role in evolutionary processes and contributes to genetic diversity and species diversity. Through comparative evaluation using simulated data, we found that HyDe and the D-statistic are powerful for detecting hybridization, while structure and ADMIXTURE sometimes fail to identify hybrids, particularly in scenarios with asymmetric parental contributions.
SYSTEMATIC BIOLOGY
(2021)
Article
Genetics & Heredity
Katarzyna Kowalczyk, Magdalena Bartnik-Glaska, Marta Smyk, Izabela Plaskota, Joanna Bernaciak, Marta Kedzior, Barbara Wisniowiecka-Kowalnik, Marta Deperas, Justyna Domaradzka, Alicja Luszczek, Daria Dutkiewicz, Agata Kozar, Dominika Grad, Magdalena Niemiec, Kamila Ziemkiewicz, Roza Magdziak, Natalia Braun-Walicka, Artur Barczyk, Maciej Geremek, Jennifer Castaneda, Anna Kutkowska-Kazmierczak, Pawel Wlasienko, Krystyna Jakubow-Durska, Marzena Debska, Anna Kucinska-Chahwan, Szymon Kozlowski, Boyana Mikulska, Tadeusz Issat, Tomasz Roszkowski, Agnieszka Nawara-Baran, Agata Runge, Anna Jakubiuk-Tomaszuk, Anna Kruczek, Ewa Kostyk, Grzegorz Pietras, Janusz Limon, Jerzy Zwolinski, Karolina Ochman, Tomasz Szajner, Piotr Wegrzyn, Miroslaw Wielgos, Maria Sasiadek, Ewa Obersztyn, Beata Anna Nowakowska
Summary: The aim of this study was to determine the suitability of the aCGH technique for prenatal diagnosis and assess the frequency of chromosomal aberrations not included in the diagnostic report. The study included 7400 prenatal cases and found a 27.2% chromosomal aberration detection rate, with 71.2% being numerical aberrations and 28.8% being structural aberrations. Clinically significant CNVs were detected in 6.8% of cases, and variants of unknown clinical significance were found in 0.7% of cases. The detection rate of clinically significant submicroscopic CNVs varied among different indications for invasive testing.
Article
Biochemistry & Molecular Biology
Bjorn Paulson, Yeonhee Shin, Akimitsu Okamoto, Yeon-Mok Oh, Jun Ki Kim, Chan-Gi Pack
Summary: RNA plays a crucial role in cellular processes, making it essential to visualize and quantify its molecular dynamics directly in living cells to understand its metabolism. In this study, fluorescence correlation methods were utilized to demonstrate the visualization and quantification of interactions between ECHO probes targeting mRNA, showing changes in fluorescence brightness and diffusion coefficient upon binding. The uptake of ECHO probes in HeLa cells for mRNA detection provides new insights into intracellular mRNA metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Meiqin Zheng, Yutong Kang, Yangyang Shen, Yi Xu, Fangyi Xu, Yanjiong Chen
Summary: In this study, a highly virulent ocular pathogen was isolated using metagenomic sequencing and bioinformatics analysis, providing important insights into the composition and genome characteristics of pathogens in endophthalmitis patients.
CURRENT MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yuki Iwasaki, Toshimichi Ikemura, Kennosuke Wada, Yoshiko Wada, Takashi Abe
Summary: This study conducted a comparative genomic study of humans and six bat species using the oligonucleotide BLSOM. The analysis revealed the Mb-level enrichment of CG dinucleotide (Mb-level CpG islands) around the termini of bat long-scaffold sequences, highlighting the potential role of unsupervised machine learning in identifying novel genome characteristics.
Review
Microbiology
Karoliny Brito Sampaio, Davi dos Santos Nascimento, Estefania Fernandes Garcia, Evandro Leite de Souza
Summary: This review discusses the recent advances in the use of fluorescent in situ hybridization coupled to flow cytometry technique in evaluating the gut microbiota. The studies have shown that this technique is effective in detecting and quantifying different bacteria groups in the gut microbiota. It has the potential to be used in evaluating the effects of foods and dietary interventions. Optimizations of the protocol could lead to further discoveries and new quantitative approaches in studying the gut microbiota.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Katarzyna Kowalczyk, Marta Smyk, Magdalena Bartnik-Glaska, Izabela Plaskota, Barbara Wisniowiecka-Kowalnik, Joanna Bernaciak, Marta Chojnacka, Magdalena Paczkowska, Magdalena Niemiec, Daria Dutkiewicz, Agata Kozar, Roza Magdziak, Wojciech Krawczyk, Grzegorz Pietras, Elzbieta Michalak, Teresa Klepacka, Ewa Obersztyn, Jerzy Bal, Beata Anna Nowakowska
Summary: Spontaneous abortion, a common issue during pregnancy, is mainly caused by chromosomal abnormalities. This study used oligonucleotide array technology to investigate 62 cases of recurrent miscarriages and found that the majority of abnormal results were related to chromosomal aberrations. Some structural abnormalities may be the cause of miscarriage.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Cell Biology
Catherine R. Armbruster, Christopher W. Marshall, Arkadiy Garber, Jeffrey A. Melvin, Anna C. Zemke, John Moore, Paula F. Zamora, Kelvin Li, Ian L. Fritz, Christopher D. Manko, Madison L. Weaver, Jordan R. Gaston, Alison Morris, Barbara Methe, William H. DePas, Stella E. Lee, Vaughn S. Cooper, Jennifer M. Bomberger
Summary: This study examined the long-term infection of Pseudomonas aeruginosa in the sinuses of CF patients, finding that highly host-adapted lineages carried mutator genotypes displaying signs of early genome degradation. The most host-adapted, genome-degraded P. aeruginosa resided in small, sparse aggregates. The research highlights the significance of infection-site biogeography in pathogen evolution.
Article
Biochemistry & Molecular Biology
Igor N. Lebedev, Tatyana Karamysheva, Eugeny A. Elisaphenko, Alexey Makunin, Daria Zhigalina, Maria E. Lopatkina, Gleb Drozdov, Aleksander D. Cheremnykh, Natalia B. Torkhova, Gulnara N. Seitova, Stanislav A. Vasilyev, Anna A. Kashevarova, Ludmila P. Nazarenko, Nikolay B. Rubtsov
Summary: The case study presented a prenatal diagnosis of a foetus with mosaic sSMC(10) and identified a ring structure derived from chromosome 10 with specific regions involved. Despite the presence of a 9 Mb euchromatin region, the sSMC did not result in clinical manifestations and a healthy girl was born. The study suggests that the ring structure of sSMCs could influence their manifestations and should be considered in genetic counselling during prenatal diagnosis.
Article
Genetics & Heredity
Laura M. Tanner, Tiina Alitalo, Vedran Stefanovic
Summary: This study retrospectively analyzed the diagnostic yield of prenatal array comparative genomic hybridization (aCGH) in high-risk pregnancies, finding an overall diagnostic yield of 15.1% in the patient cohort, with the potential for higher yields in fetuses with multiple structural anomalies. However, a relatively high number of copy number variants (CNVs) with unclear correlation to fetal ultrasound findings was observed, highlighting the importance of adequate pre- and posttest counseling.
PRENATAL DIAGNOSIS
(2021)
Article
Biochemistry & Molecular Biology
Rafal Bartoszewski, Magdalena Gebert, Anna Janaszak-Jasiecka, Aleksandra Cabaj, Jaroslaw Kroliczewski, Sylwia Bartoszewska, Aleksandra Sobolewska, David K. Crossman, Renata Ochocka, Wojciech Kamysz, Leszek Kalinowski, Michal Dabrowski, James F. Collawn
Article
Materials Science, Biomaterials
Piotr M. Skowron, Natalia Krawczun, Joanna Zebrowska, Dania Krefft, Olga Zolnierkiewicz, Marta Bielawa, Joanna Jezewska-Frackowiak, Lukasz Janus, Malgorzata Witkowska, Malgorzata Palczewska, Adriana Schumacher, Anna Wardowska, Milena Deptula, Artur Czupryn, Piotr Mucha, Arkadiusz Piotrowski, Pawel Sachadyn, Sylwia Rodziewicz-Motowidlo, Michal Pikula, Agnieszka Zylicz-Stachula
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Jan P. Dumanski, Jonatan Halvardson, Hanna Davies, Edyta Rychlicka-Buniowska, Jonas Mattisson, Behrooz Torabi Moghadam, Noemi Nagy, Kazimierz Weglarczyk, Karolina Bukowska-Strakova, Marcus Danielsson, Pawel Olszewski, Arkadiusz Piotrowski, Erin Oerton, Aleksandra Ambicka, Marcin Przewoznik, Lukasz Belch, Tomasz Grodzicki, Piotr L. Chlosta, Stefan Imreh, Vilmantas Giedraitis, Lena Kilander, Jessica Nordlund, Adam Ameur, Ulf Gyllensten, Asa Johansson, Alicja Jozkowicz, Maciej Siedlar, Alicja Klich-Raczka, Janusz Jaszczynski, Stefan Enroth, Jaroslaw Baran, Martin Ingelsson, John R. B. Perry, Janusz Rys, Lars A. Forsberg
Summary: Epidemiological investigations have shown that mosaic loss of chromosome Y (LOY) in leukocytes is associated with increased mortality and morbidity in men. Studies on sorted and single cells revealed significant variations in the presence of LOY across cell types and individuals, suggesting potential pleiotropic effects on gene expression and immune function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Janusz Limon, Krzysztof Mrozek
Summary: Dr. Albert de la Chapelle, a pioneer in human genetics and cytogenetics, is celebrated in this brief article for his numerous scientific achievements.
JOURNAL OF APPLIED GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Jaroslaw Mazuryk, Izabela Puchalska, Kamil Kozinski, Magdalena J. Slusarz, Jaroslaw Ruczynski, Piotr Rekowski, Piotr Rogujski, Rafal Platek, Marta Barbara Wisniewska, Arkadiusz Piotrowski, Lukasz Janus, Piotr M. Skowron, Michal Pikula, Pawel Sachadyn, Sylwia Rodziewicz-Motowidlo, Artur Czupryn, Piotr Mucha
Summary: Ischemic stroke disrupts cerebral blood flow due to brain tissue ischemia and hypoxia. A study using a rat primary neural cell culture model found that the cell-penetrating peptide PTD4 showed significant pro-survival effects in acute ischemic stroke scenarios, likely due to its ability to adopt a helical structure in a membrane-mimicking environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Arkadiusz Piotrowski, Magdalena Koczkowska, Andrzej B. Poplawski, Rafal Bartoszewski, Jaroslaw Kroliczewski, Alina Mieczkowska, Alicia Gomes, Michael R. Crowley, David K. Crossman, Yunjia Chen, Ping Lao, Eduard Serra, Meritxell C. Llach, Elisabeth Castellanos, Ludwine M. Messiaen
Summary: Constitutional pathogenic variants in genes LZTR1 or SMARCB1 were identified in a high percentage of familial and sporadic schwannomatosis cases, with additional novel variants and potential predisposing candidate genes revealed through extensive sequencing.
Article
Biochemistry & Molecular Biology
Justyna Stefanowicz-Hajduk, Anna Hering, Magdalena Gucwa, Katarzyna Sztormowska-Achranowicz, Mariusz Kowalczyk, Agata Soluch, J. Renata Ochocka
Summary: Kalanchoe species have anti-inflammatory, antioxidant, and analgesic properties. In this study, the phytochemical composition of Kalanchoe daigremontiana water extract was analyzed and its cytotoxic and antioxidant activities were evaluated. The extract contained bufadienolide compounds and exhibited antiproliferative and non-apoptotic cell death-inducing effects. Additionally, it displayed antioxidant activity.
Article
Multidisciplinary Sciences
Natalia Filipowicz, Kinga Drezek, Monika Horbacz, Agata Wojdak, Jakub Szymanowski, Edyta Rychlicka-Buniowska, Ulana Juhas, Katarzyna Duzowska, Tomasz Nowikiewicz, Wiktoria Stankowska, Katarzyna Chojnowska, Maria Andreou, Urszula Lawrynowicz, Magdalena Wojcik, Hanna Davies, Ewa Srutek, Michal Bienkowski, Katarzyna Milian-Ciesielska, Marek Zdrenka, Aleksandra Ambicka, Marcin Przewoznik, Agnieszka Harazin-Lechowska, Agnieszka Adamczyk, Jacek Kowalski, Dariusz Bala, Dorian Wisniewski, Karol Tkaczynski, Krzysztof Kamecki, Marta Drzewiecka, Pawel Wronski, Jerzy Siekiera, Izabela Ratnicka, Jerzy Jankau, Karol Wierzba, Jaroslaw Skokowski, Karol Polom, Mikolaj Przydacz, Lukasz Belch, Piotr Chlosta, Marcin Matuszewski, Krzysztof Okon, Olga Rostkowska, Andrzej Hellmann, Karol Sasim, Piotr Remiszewski, Marek Sierzega, Stanislaw Hac, Jaroslaw Kobiela, Lukasz Kaska, Michal Jankowski, Diana Hodorowicz-Zaniewska, Janusz Jaszczynski, Wojciech Zegarski, Wojciech Makarewicz, Rafal Peksa, Joanna Szpor, Janusz Rys, Lukasz Szylberg, Arkadiusz Piotrowski, Jan P. Dumanski
Summary: The progress in translational cancer research relies on access to well-characterized samples from a representative number of patients and controls. Our biobanking focuses on exploring pathogenic gene variants that may predispose to cancer in non-tumoral tissue. The collection includes various types of cancer, with breast, colorectal, and prostate carcinoma being the most common diagnoses. Additionally, we have developed program packages to manage patient registration and clinical data, making our biobank a model for dispersed biobanking from multiple hospitals. This resource is important for understanding the genetic mechanisms underlying common cancers and can be utilized by other research groups.
Article
Genetics & Heredity
Katarzyna Kowalczyk, Magdalena Bartnik-Glaska, Marta Smyk, Izabela Plaskota, Joanna Bernaciak, Marta Kedzior, Barbara Wisniowiecka-Kowalnik, Marta Deperas, Justyna Domaradzka, Alicja Luszczek, Daria Dutkiewicz, Agata Kozar, Dominika Grad, Magdalena Niemiec, Kamila Ziemkiewicz, Roza Magdziak, Natalia Braun-Walicka, Artur Barczyk, Maciej Geremek, Jennifer Castaneda, Anna Kutkowska-Kazmierczak, Pawel Wlasienko, Krystyna Jakubow-Durska, Marzena Debska, Anna Kucinska-Chahwan, Szymon Kozlowski, Boyana Mikulska, Tadeusz Issat, Tomasz Roszkowski, Agnieszka Nawara-Baran, Agata Runge, Anna Jakubiuk-Tomaszuk, Anna Kruczek, Ewa Kostyk, Grzegorz Pietras, Janusz Limon, Jerzy Zwolinski, Karolina Ochman, Tomasz Szajner, Piotr Wegrzyn, Miroslaw Wielgos, Maria Sasiadek, Ewa Obersztyn, Beata Anna Nowakowska
Summary: The aim of this study was to determine the suitability of the aCGH technique for prenatal diagnosis and assess the frequency of chromosomal aberrations not included in the diagnostic report. The study included 7400 prenatal cases and found a 27.2% chromosomal aberration detection rate, with 71.2% being numerical aberrations and 28.8% being structural aberrations. Clinically significant CNVs were detected in 6.8% of cases, and variants of unknown clinical significance were found in 0.7% of cases. The detection rate of clinically significant submicroscopic CNVs varied among different indications for invasive testing.
Article
Chemistry, Medicinal
Pawel Sosnowski, Piotr Sass, Paulina Slonimska, Rafal Platek, Jolanta Kaminska, Jakub Baczynski Keller, Piotr Mucha, Grazyna Peszynska-Sularz, Artur Czupryn, Michal Pikula, Arkadiusz Piotrowski, Lukasz Janus, Sylwia Rodziewicz-Motowidlo, Piotr Skowron, Pawel Sachadyn
Summary: The ear pinna is a complex tissue consisting of various components such as dermis, cartilage, muscles, vessels, and nerves. Healing of ear pinna wounds can vary among different species, with some mammals having the ability to regenerate while others do not. In this study, researchers tested the effects of different bioactive agents on wound closure in mice and identified several substances that can enhance regeneration. They also observed the presence of nerve fiber networks and blood vessels in the regenerated areas of the ear pinna.
Article
Oncology
Anna Kostecka, Tomasz Nowikiewicz, Pawel Olszewski, Magdalena Koczkowska, Monika Horbacz, Monika Heinzl, Maria Andreou, Renato Salazar, Theresa Mair, Piotr Madanecki, Magdalena Gucwa, Hanna Davies, Jaroslaw Skokowski, Patrick G. Buckley, Rafal Peksa, Ewa Srutek, Lukasz Szylberg, Johan Hartman, Michal Jankowski, Wojciech Zegarski, Irene Tiemann-Boege, Jan P. Dumanski, Arkadiusz Piotrowski
Summary: The mammary gland undergoes hormonally stimulated cycles of proliferation, lactation, and involution. This study investigated the DNA sequence variants in the normal mammary gland of breast cancer patients and found subclonal somatic pathogenic variants, indicating clonal expansion. The results raise questions about the oncogenic potential in non-tumorous mammary gland tissue of breast-conserving surgery patients.
Article
Biochemistry & Molecular Biology
Anna Hering, Justyna Stefanowicz-Hajduk, Rafal Halasa, Marta Olech, Renata Nowak, Piotr Kosinski, J. Renata Ochocka
Summary: This study analyzed water and ethanol extracts from young leaves and stems of the European dewberry for the first time, revealing their antioxidant and biological activity. The extracts showed high antioxidant activity, inhibited hyaluronidase activity, and exhibited antimicrobial effects against certain bacteria.
Article
Biochemistry & Molecular Biology
Justyna Stefanowicz-Hajduk, Anna Hering, Magdalena Gucwa, Monika Czerwinska, J. Renata Ochocka
Summary: Steroidal saponins, such as yamogenin, exhibit cytotoxic and apoptotic effects on ovarian cancer cells. The compound induces cell death through both the extrinsic and mitochondrial-intrinsic pathways. These findings suggest that yamogenin may serve as a potential therapeutic agent for ovarian cancer.
Article
Multidisciplinary Sciences
Magdalena Koczkowska, Marcin Jakalski, Dorota Birkholz-Walerzak, Anna Kostecka, Mariola Iliszko, Magdalena Wojcik, Krzysztof Lewandowski, Katarzyna Milska-Musa, Patrick G. Buckley, Kinga Drezek, Ulana Juhas, Ewa Kuziemska, Agnieszka Maciejewska, Ryszard Pawlowski, Bartosz Wasag, Natalia Filipowicz, Katarzyna Chojnowska, Urszula Lawrynowicz, Jan P. Dumanski, Beata S. Lipska-Zietkiewicz, Jakub Mieczkowski, Arkadiusz Piotrowski
Summary: Numeric sex chromosome abnormalities are associated with increased cancer risk. This study reports a rare mosaic karyotype case and provides comprehensive genetic and biological analysis suggesting immune cell differences and gene deregulation, recommending long-term hematological surveillance for the patient.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Anna Hering, Justyna Stefanowicz-Hajduk, Szymon Dziomba, Rafal Halasa, Radoslaw Krzemieniecki, Subrahmanyam Sappati, Maciej Baginski, Jadwiga Renata Ochocka
Summary: This study evaluated the influence of mangiferin on tyrosinase and found that it inhibits tyrosinase activity in a dose-dependent manner. The interaction between mangiferin and tyrosinase was confirmed through capillary electrophoresis and molecular docking studies.
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)