Article
Nutrition & Dietetics
Rui Azevedo, Davide Gennaro, Mary Duro, Edgar Pinto, Agostinho Almeida
Summary: Previous studies have shown that haemodialysis patients have an increased risk of trace element imbalances. In this study, both serum and whole blood concentrations of a wide panel of trace elements in haemodialysis patients were determined and compared with those of a control group. The results indicate that chronic haemodialysis may affect intra- and extracellular blood compartments differently, leading to significant trace element imbalances.
Article
Chemistry, Analytical
Andrew K. Schug, Diane Beauchemin
Summary: A new method was developed for the direct analysis of rock samples to ensure accurate results, showing that using a specific concentration of surfactant can improve the reproducibility of sample introduction.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2021)
Article
Chemistry, Applied
Semih Kara, Dotse Selali Chormey, Ahmet Saygilar, Sezgin Bakirdere
Summary: This study utilized a combination of high-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) to separate and quantify six arsenic species. The method demonstrated high sensitivity and accuracy, with limits of detection ranging between 0.14-0.29 ng/mL. Spike recovery experiments on rice samples validated the method's applicability to complex matrices, with recovery results ranging between 93 and 109%. Triplicate measurements on all spiked samples recorded percent relative standard deviation values below 10%, indicating the reliability and precision of the method.
Article
Biochemistry & Molecular Biology
Peter Heitland, Helmut D. Koester
Summary: This study extends biomonitoring to 73 elements in clinically relevant specimens such as blood, serum, erythrocytes, and urine, providing valuable information for occupational or environmental hygienists, toxicologists, and clinical chemists due to the high number of determined elements and presented concentration ranges.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2021)
Article
Environmental Sciences
Rajendran Shalini, Geevaretnam Jeyasekaran, Robinson Jeya Shakila, Ulaganathan Arisekar
Summary: The study found that the main accumulation organs of trace elements in commercially important fish along the southeast coast of India are different, and the concentration of toxic trace elements in the edible portion is lower than the international legislation limits.
MARINE POLLUTION BULLETIN
(2021)
Article
Food Science & Technology
Lena Ruzik, Malgorzata Jakubowska
Summary: The popularity of plant-based food products has grown in response to the increasing demand for plant foods. This study analyzed the total arsenic content and its species in plant-based beverages purchased in Poland. The research validated a method for analyzing arsenic using high-performance liquid chromatography combined with inductively coupled plasma mass spectrometry.
Article
Spectroscopy
Davide Spanu, Stefano Fantinuoli, Gilberto Binda, Laura Rampazzi, Damiano Monticelli, Sandro Recchia
Summary: This study reports the development and validation of a sensitive, fast, high throughput and cost-effective method for the selective determination of Sb(III) and Sb(V) in water samples. The method separates the two species using a homemade cation exchange column and detects them using ICP-MS. The method offers very low detection limits, fast analysis time, and favorable instrumental configuration.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Omer Faruk Kocak, Fatma Betul Ozgeris, Emine Parlak, Yucel Kadioglu, Neslihan Yuce, Mehmet Emrah Yaman, Ebubekir Bakan
Summary: This study investigated serum Zn and Se concentrations in COVID-19 patients of different severity levels, and found that as the disease severity increased, the serum Zn and Se levels decreased.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ayse Sahin, Elif Ozturk Er, Ersoy Oz, Zeynep Yildiz Yildirmak, Sezgin Bakirdere
Summary: The study found significant differences in the concentrations of Al, K, Sn, and Sb elements in the serum of patients with beta-thalassemia major compared to the control group. Higher levels of Al and Sb and lower levels of K and Sn elements were observed in patients, indicating an altered profile of serum trace element concentrations. Further research is needed to evaluate the potential of trace elements as biomarkers and for managing their levels to reduce related complications.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Chemistry, Analytical
P. F. Rodriguez, R. M. Martin-Aranda, J. L. Lopez Colon, J. H. de Mendoza
Summary: A novel HPLC-ICP-MS arsenic speciation methodology was developed using ammonium acetate, applicable to six arsenic species. The method covered the most predominant species in toxicology field and addressed typical problems from traditional methods. Chromatographic and detector optimization ensured low LOQs for each species, making it useful for sub ng mL(-1) arsenic exposure assessments.
Article
Chemistry, Applied
Ghaya Alchoubassi, Katarzyna Kinska, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar
Summary: Coconut water has been shown to contain essential elements such as iron, copper, manganese, and zinc, with concentrations varying depending on the origin and maturity of the coconut. Speciation analysis revealed the presence of various metal species in coconut water, each contributing differently to the total element concentrations.
Article
Chemistry, Analytical
Tamara Fernandez-Bautista, Beatriz Gomez-Gomez, Roberto Palacin-Garcia, Emma Gracia-Lor, Teresa Perez-Corona, Yolanda Madrid
Summary: The distribution of Se and Hg in different protein fractions extracted from muscle tissues of tuna, swordfish, and salmon (wild vs. farmed) was investigated for the first time. The study revealed that Se and Hg were associated with proteins of specific molecular weights, with differences observed between farmed and wild salmon. Additionally, Se speciation studies showed SeMeSeCys as the primary selenium species found in the proteins analyzed.
Article
Environmental Sciences
Marcus von der Au, Tristan Zimmermann, Ulrike Kleeberg, Wolf von Tuempling, Daniel Proefrock
Summary: The study analyzed trace elements in surface sediment samples collected in 2014 to assess the pollution level and changes in the Wadden Sea. Cluster analysis and principal component analysis revealed three distinct sub-catchments, and discriminant analysis confirmed the classification. The study also demonstrated the representative nature of the sampling strategy for sediment characterization.
MARINE POLLUTION BULLETIN
(2022)
Article
Food Science & Technology
Federica Gulino, Elisa Cala, Christian Cozzani, Lorenzo Vaccari, Matteo Oddone, Maurizio Aceto
Summary: Honey is highly valued for its beneficial properties, but it is also susceptible to counterfeiting. Analytical methods are being developed to verify its origin and authenticity using trace- and ultra-trace elements. Lanthanides have been found to be particularly useful for tracing the link between honey and the soil it originates from. The study used ICP-MS and ICP-OES analysis to investigate the traceability of 17 different honey chains, and found that while the soil fingerprint remains unchanged, there is slight fractionation of heavier lanthanides from flowers to honey.
Article
Environmental Sciences
Marta Herrero, Joaquim Rovira, Neus Gonzalez, Montse Marques, Fernando Barbosa, Jordi Sierra, Jose L. Domingo, Marti Nadal, Marilia Cristina Oliveira Souza
Summary: The interest in determining the chemical composition of textile products has increased due to toxicological issues and environmental concerns. This study analyzed the levels of twenty trace elements in clothing items commercialized in Spain and evaluated the potential health implication of dermal exposure in early life. Aluminum, zinc, and titanium showed the highest concentrations, while chromium was found in high levels in black polyester. Dermal exposure to titanium was associated with a hazard quotient higher than the threshold value, indicating potential risks for pregnant women and newborns.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Robertina Giacconi, Patrizia D'Aquila, Marta Balietti, Cinzia Giuli, Marco Malavolta, Francesco Piacenza, Laura Costarelli, Demetrio Postacchini, Giuseppe Passarino, Dina Bellizzi, Mauro Provinciali
Summary: Microbial dysbiosis leads to alterations in gut barrier and bacterial translocation, resulting in increased blood bacterial DNA that promotes peripheral and neuro inflammation and contributes to cognitive impairment. It also affects the production of brain-derived neurotrophic factor, which plays a role in the etiopathogenesis of Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Francesco Piacenza, Antonio Cherubini, Roberta Galeazzi, Maurizio Cardelli, Robertina Giacconi, Elisa Pierpaoli, Francesca Marchegiani, Fiorella Marcheselli, Rina Recchioni, Tiziana Casoli, Elisabetta Farnocchia, Beatrice Bartozzi, Belinda Giorgetti, Pierpaolo Stripoli, Anna Rita Bonfigli, Massimiliano Fedecostante, Fabio Salvi, Adolfo Pansoni, Mauro Provinciali, Fabrizia Lattanzio
Summary: This study assessed the performance of the rapid molecular VitaPCR(TM) test in older adult patients at the Emergency Department of two Italian hospitals. The VitaPCR(TM) showed good agreement with the RT-PCR and had a sensitivity of 83.4% and specificity of 99.9% for detecting SARS-CoV-2. It can be used for rapid management of patients in the Emergency Department, but a negative result from RT-PCR is advisable before admitting a patient to a regular ward.
Article
Biochemistry & Molecular Biology
Robertina Giacconi, Maurizio Cardelli, Francesco Piacenza, Elisa Pierpaoli, Elisabetta Farnocchia, MirKo Di Rosa, Anna Rita Bonfigli, Tiziana Casoli, Francesca Marchegiani, Fiorella Marcheselli, Rina Recchioni, Pierpaolo Stripoli, Roberta Galeazzi, Antonio Cherubini, Massimiliano Fedecostante, Riccardo Sarzani, Chiara Di Pentima, Piero Giordano, Roberto Antonicelli, Mauro Provinciali, Fabrizia Lattanzio
Summary: This study aimed to investigate the association between cytomegalovirus (CMV) reactivation and inflammation as well as in-hospital mortality in older patients with severe COVID-19. Among 156 geriatric COVID-19 patients, 12.2% showed CMV reactivation. CMV reactivation was found to be an independent risk factor for in-hospital mortality in COVID-19 patients younger than 87 years old.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maria Elisa Giuliani, Veronica Barbi, Giorgia Bigossi, Serena Marcozzi, Robertina Giacconi, Maurizio Cardelli, Francesco Piacenza, Fiorenza Orlando, Elena Ciaglia, Monica Cattaneo, Alessia Mongelli, Carlo Gaetano, Mauro Provinciali, Annibale Alessandro Puca, Marco Malavolta
Summary: This study found that the homozygous genotype of the Longevity-Associated Variant (LAV) in BPIFB4 is enriched in long-living individuals and gene transfer of LAV-BPIFB4 in aged mice showed improvements in aging biomarkers and health. These findings support the use of LAV-BPIFB4 gene therapy to induce beneficial effects on epigenetic mechanisms associated with aging and frailty in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Robertina Giacconi, Francesco Piacenza, Valentina Aversano, Michele Zampieri, Alexander Buerkle, Maria Moreno Villanueva, Martijn E. T. Dolle, Eugene Jansen, Tilman Grune, Efstathios S. Gonos, Claudio Franceschi, Miriam Capri, Birgit Weinberger, Ewa Sikora, Olivier Toussaint, Florence Debacq-Chainiaux, Wolfgang Stuetz, Pieternella Eline Slagboom, Juergen Bernhardt, Maria Luisa Fernandez-Sanchez, Mauro Provinciali, Marco Malavolta
Summary: Plasma selenium levels are influenced by age, sex, nutrition, and inflammation. Inadequate selenium intake can accelerate aging and increase the risk of age-related diseases.
Article
Nutrition & Dietetics
Francesco Piacenza, Robertina Giacconi, Laura Costarelli, Marco Malavolta
Summary: This preliminary study investigated the fractional Zn absorption (FZA) after the supplementation of three different Zn complexes on eight healthy volunteers. The results showed that the FZA of Zn-Aspartate was significantly higher than other forms of Zn, and the FZA of Zn-Gluconate was significantly higher than ZnSO4. These results suggest that using Zn-Aspartate in individuals at risk of Zn deficiency could improve the Zn absorption rate.
Article
Cell Biology
Serena Marcozzi, Maria Elisa Giuliani, Giovanni Lai, Robertina Giacconi, Francesco Piacenza, Giorgia Bigossi, Marco Malavolta
Summary: This perspective review explores the mechanisms and implications of senescent cell spreading on frailty, as well as its therapeutic development. The focus is on emerging age-related biological factors, such as microbiome and virome alterations, and their significant contribution to the accumulation rate of senescent cells. These factors particularly affect the musculoskeletal system and cerebral functions, two key domains of frailty. Understanding these mechanisms can lead to the development of tailored interventions for diverse patients.
Article
Microbiology
Robertina Giacconi, Rossella Donghia, Graziana Arborea, Maria Teresa Savino, Mauro Provinciali, Fabrizia Lattanzio, Giusy Rita Caponio, Sergio Coletta, Antonia Bianco, Maria Notarnicola, Caterina Bonfiglio, Giuseppe Passarino, Patrizia DAquila, Dina Bellizzi, Pasqua Letizia Pesole
Summary: This study found that patients with colorectal cancer have higher levels of bacterial DNA in their plasma compared to healthy individuals, and this is associated with the size of the tumor mass.
Review
Geriatrics & Gerontology
Serena Marcozzi, Giorgia Bigossi, Maria Elisa Giuliani, Robertina Giacconi, Francesco Piacenza, Maurizio Cardelli, Dario Brunetti, Agnese Segala, Alessandra Valerio, Enzo Nisoli, Fabrizia Lattanzio, Mauro Provinciali, Marco Malavolta
Summary: This study provides a comprehensive evaluation of the role of senescent cells in driving inflammation and frailty. The results indicate that the burden of senescent cells related to musculoskeletal and cerebral health has the strongest causal link to frailty. These findings are important for understanding the role of cellular senescence in frailty and developing personalized therapies.
Article
Biochemistry & Molecular Biology
Raffaella Lazzarini, Maria Fiorella Tartaglione, Veronica Ciarapica, Francesco Piva, Matteo Giulietti, Gianluca Fulgenzi, Margherita Martelli, Caterina Ledda, Ermanno Vitale, Marco Malavolta, Lory Santarelli, Massimo Bracci
Summary: This study found that high-irradiance blue or red light exposure reduces the viability of keratinocytes and induces cell death and cell cycle arrest. It also affects mitochondrial density and morphology. Proteomic analysis identified the key roles of TXNRD1 and AKR1C3 in the cellular response to high-irradiance blue or red light exposure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
