Article
Chemistry, Physical
Fandi Oktasendra, Arben Jusufi, Andrew R. Konicek, Mohsen S. Yeganeh, Jack R. Panter, Halim Kusumaatmaja
Summary: We use a phase field simulation method to investigate liquid filling on grooved surfaces, considering both short-range and long-range liquid-solid interactions. Different wetting states are captured, and complex disjoining pressure profiles are observed. The filling transition is reversible for complete wetting and shows hysteresis for partial and pseudo-partial wetting. The critical pressure for the filling transition follows the Kelvin equation for complete and partial wetting scenarios, and distinct morphological pathways are identified for pseudo-partial wetting.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Biochemical Research Methods
Raymond B. Yu, Joselito P. Quirino
Summary: The application of ionic liquids as additives in EKC plays an important role in chiral separations, as evidenced by numerous research articles. Studies show that the use of ionic liquids can improve separation efficiency, with further research needed to validate their mechanisms of action.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Computer Science, Artificial Intelligence
Chaoyu Gong, Yang You
Summary: Partial multi-view clustering (PMVC) is a popular topic in data mining, but existing algorithms often require the number of clusters as hyperparameters and can only create hard partitions, ignoring ambiguity and uncertainty in object assignment. To address this, we propose a novel self-filling evidential clustering algorithm (SFPMEC) that provides a human-readable chart and derives a credal partition under the framework of evidence theory, improving fault tolerance and gaining insights into the data structure.
EXPERT SYSTEMS WITH APPLICATIONS
(2024)
Article
Biochemical Research Methods
Hui Jiang, Hao Zhang, Shi-Jun Yin, Min Lu, Xu Wang, Feng-Qing Yang
Summary: This study focused on profiling lipid-water partition coefficients of drugs using liposome electrokinetic chromatography (LEKC). Results showed that DSPC/Chol liposomes had a better linear relationship with the Caco-2 cell model compared to SPC/Chol liposomes, indicating their suitability for simulating cell membranes. The developed LEKC proved to be an effective model for measuring the log K-L/W of drugs.
Article
Biochemical Research Methods
Katerina Cokrtova, Tomas Krizek
Summary: A method for purity control of newly synthesized lactic acid-based liquid crystals has been developed using electrokinetic chromatography. This method shows viability in separating impurities from liquid crystals, with lower operational costs compared to other chromatography methods.
Article
Chemistry, Analytical
Raymond B. Yu, Joselito P. Quirino
Summary: Pseudophase microextraction (PPME) is presented as a simple in-line sample concentration technique in micellar electrokinetic chromatography (MEKC). Compared to popular electric-field driven stacking techniques in MEKC, such as sweeping, PPME is pressure-driven and can greatly improve peak heights for target analytes. The entire capillary is loaded with the sample and undergoes pressure-driven concentration, leading to effective detection of low levels of analytes in drinking water and natural water samples.
ANALYTICA CHIMICA ACTA
(2022)
Review
Biochemistry & Molecular Biology
Raymond B. Yu, Joselito P. Quirino
Summary: This review examines the use of bile salts as chiral selectors in chiral micellar electrokinetic chromatography (MEKC) over the past 20 years, discussing their aggregation in chiral recognition and separation, as well as their use in combination with other chiral selectors. Advanced techniques like partial-filling MEKC, stacking, and single-drop microextraction are considered, along with potential applications to real samples such as enantiomeric impurity analysis.
Article
Chemistry, Analytical
Ang Li, Song Xue, Siru Ren, Yu Xu, Qi Zhang
Summary: Research on the application of deep eutectic solvents (DESs) in capillary electrophoresis (CE) is still in the early stage, and there is controversy over their true role. This study established a CE system using a hydrophobic DES as a pseudo-stationary phase (PSP). The hydrophobic DES formed stable aggregates and exhibited impressive selectivities through a hydrophobic mechanism.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Kanji Miyabe
Summary: Moment equations were developed for partial filling capillary electrophoresis systems to study solute dissolution phenomena by spherical molecular assemblies or intermolecular interactions. The effectiveness of the moment equations was demonstrated by analyzing partial filling CE behavior and simulation results. These equations provide theoretical bases for studying solute permeation kinetics and reaction kinetics at the interface of spherical molecular assemblies.
Article
Biochemistry & Molecular Biology
Anna Saldan, Malgorzata Krol, Daria Smigiel-Kaminska, Michal Wozniakiewicz, Pawel Koscielniak
Summary: The study developed a method for extracting dispersed dyes from polyester fibers and successfully applied it to the differentiation analysis of real samples. The research suggests that the method has promising potential in discriminant analysis and forensic practice.
Article
Chemistry, Analytical
Henri Ravald, Amin Hedayati Moghaddam, Shishir Jaikishan, Marine Lavainne, Susanne K. Wiedmer
Summary: Liposome capillary electrokinetic chromatography was used to study the interactions between three beta-blockers of different hydrophobicity and liposome solutions. The liposome solutions, with different compositions of lipids, were designed by a response surface methodology-central composite design approach. Statistical analysis was conducted to determine the optimal operational conditions. Additionally, nanoplasmonic sensing experiments were carried out to further investigate the interactions between the beta-blockers and the liposomes.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Wolfgang Thormann
Summary: The study evaluated the impact of complexation constant and the mobility of formed diastereomeric complex on stereoisomer separation in the presence of a neutral chiral selector. The findings indicate that differences in these parameters affect the efficiency of chiral separation.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Environmental Sciences
Anna W. Sobanska
Summary: In this study, Y RP-18 TLC chromatography was used to evaluate the environmental impact of several cosmetic raw materials, showing that chromatographic parameters are good predictors of compound mobility in soil or affinity for aquatic organisms. Parameters for THF, dioxane, and acetone may be useful for investigating lipophilic compounds, with additional descriptors such as PSA significantly influencing correlation quality.
Article
Chemistry, Analytical
Henri Ravald, Shishir Jaikishan, Jorgen Samuelsson, Arina Sukhova, Veronika Solinova, Torgny Fornstedt, Vaclav Kasicka, Susanne K. Wiedmer
Summary: Toxicity of beta-blockers is a common cause of poison-induced cardiogenic shock worldwide. In this study, the interactions between a set of beta-blockers and intralipid emulsion (ILE) were investigated. The results showed that the binding constants and adsorption constants of the beta-blocker-ILE complexes were strongly related to the hydrophobicity of the beta-blockers. Less hydrophobic beta-blockers showed weaker interactions with ILE, suggesting the potential use of ILE for capturing these compounds in cases of overdose. Further investigation is warranted for using ILE in the treatment of toxicities caused by a wider range of beta-blockers.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Hanan Alatawi, Anna Hogan, Ibtihaj Albalawi, Samia Alsefri, Eric Moore
Summary: Recently, the increased use of non-steroidal anti-inflammatory drugs (NSAIDs) in humans and animals has raised concerns about their impact on aquatic environments. In this study, a highly efficient and sensitive micellar electrokinetic chromatography (MEKC) method was developed to detect five NSAIDs in environmental water samples. The optimized method showed excellent separation using a borate buffer, SDS, and methanol, and achieved high theoretical plates and great sensitivity for NSAIDs. Furthermore, the solid phase extraction method was successfully validated for wastewater samples with excellent recovery rates.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Xinpei Li, Hangzhen Lan, Kari Hartonen, Matti Jussila, Xinghua Wang, Marja-Liisa Riekkola
JOURNAL OF SEPARATION SCIENCE
(2020)
Article
Chemistry, Analytical
Evgen Multia, Thanaporn Liangsupree, Matti Jussila, Jose Ruiz-Jimenez, Marianna Kemell, Marja-Liisa Riekkola
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Teemu Tomberg, Niko Vuorio, Tuomas Hieta, Matti Jussila, Kari Hartonen, Markku Vainio, Tommi Mikkonen, Juha Toivonen, Marja-Liisa Riekkola, Lauri Halonen, Markus Metsala
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Aijie Liu, Lars Gedda, Martin Axelsson, Mariia Pavliuk, Katarina Edwards, Leif Hammarstrom, Haining Tian
Summary: The panchromatic ternary polymer dots show efficient charge separation and high stability, leading to enhanced light absorption and improved photocatalytic yield.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Pharmacology & Pharmacy
Dileep Urimi, Ronja Widenbring, Raul Oswaldo Perez Garcia, Lars Gedda, Katarina Edwards, Thorsteinn Loftsson, Nicolaas Schipper
Summary: The lipid nanocapsules loaded with DF003 showed favorable characteristics in terms of particle size, entrapment efficiency, and stability, indicating their potential as drug delivery vehicles. The upscaling and continuous production process further demonstrated the quality and industrial viability of the nanocapsules.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Philipp Grad, Victor Agmo Hernandez, Katarina Edwards
Summary: This study investigated methods to minimize artifacts observed in experiments measuring spontaneous leakage from cholesterol-containing liposomes, showing that the choice of cuvette material significantly influences the results. Side processes at the solution-cuvette interface, such as attaching and spreading of liposomes, likely contribute to discrepancies in leakage profiles. Modification of quartz cuvettes with adsorbed PEG8000 was found to be suitable for these experiments, while other cuvette materials performed poorly.
JOURNAL OF LIPOSOME RESEARCH
(2022)
Article
Environmental Sciences
Hangzhen Lan, Jose Ruiz-Jimenez, Yevgeny Leleev, Giorgia Demaria, Matti Jussila, Kari Hartonen, Marja-Liisa Riekkola
Summary: The study utilized a second generation air sampling drone system to collect atmospheric air samples at SMEAR II station and Qvidja farm in Finland, providing comprehensive chemical characterization. A total of 347 VOCs were identified, showing clear differences in average concentrations between gas phase and aerosols as well as among different sampling sites and heights.
Article
Environmental Sciences
Eka Dian Pusfitasari, Jose Ruiz-Jimenez, Ilmari Heiskanen, Matti Jussila, Kari Hartonen, Marja-Liisa Riekkola
Summary: A wide variety of nitrogen-containing compounds in the environment contribute to air pollution and new particle formation, which eventually impact human health and the climate. The study utilized titanium hydrogen phosphate-modified sorbent material for selective collection of nitrogen-containing compounds, and investigated sampling accessories for improving selectivity and removing particles. By employing aerial drones with miniaturized air sampling systems, the research successfully collected and analyzed more than twenty nitrogen-containing compounds in gas phase and aerosol particles.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Medicine, Research & Experimental
Dileep Urimi, Maja Hellsing, Najet Mahmoudi, Christopher Soderberg, Ronja Widenbring, Lars Gedda, Katarina Edwards, Thorsteinn Loftsson, Nicolaas Schipper
Summary: Lipid nanocapsules (LNCs) with core-shell structure were studied using various techniques, and it was found that drug loading does not alter the overall structure of LNCs. However, the presence of the drug leads to an increase in the thickness of the shell while the core size remains unchanged.
MOLECULAR PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Ondrej Sedlacek, Valentin Bardoula, Elina Vuorimaa-Laukkanen, Lars Gedda, Katarina Edwards, Aurel Radulescu, Grigoriy A. Mun, Yong Guo, Junnian Zhou, Hongbo Zhang, Veronique Nardello-Rataj, Sergey Filippov, Richard Hoogenboom
Summary: This study provides an in-depth analysis of micelles based on amphiphilic gradient copolymers and compares them to diblock copolymer micelles as potential drug delivery systems. The results show that gradient copolymers can provide stable drug nanoformulations with comparable drug loadings to block copolymer systems and benefit from easier synthesis.
Article
Chemistry, Multidisciplinary
Mariia V. Pavliuk, Marco Lorenzi, Dustin R. Morado, Lars Gedda, Sina Wrede, Sara H. Mejias, Aijie Liu, Moritz Senger, Starla Glover, Katarina Edwards, Gustav Berggren, Haining Tian
Summary: This study introduces the interaction between polymer dots (Pdots) and HydA1 [FeFe]-hydrogenase in a biohybrid assembly, demonstrating efficient charge separation and hydrogen evolution. The attachment of hydrogenase on Pdots leads to a stable biohybrid assembly, enabling continuous hydrogen production for days.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biophysics
Philipp Grad, Victor Agmo Hernandez, Katarina Edwards
Summary: Recent studies have shown that interactions between liposomes and solid materials commonly found in cuvettes used for spectroscopic measurements can lead to detrimental changes in liposome structure and compromise the measurement accuracy. This study investigates the use of quartz cuvettes passivated with supported lipid bilayers (SLBs) to prevent these unwanted interactions. The results demonstrate that this strategy significantly improves the reproducibility and accuracy of leakage measurements.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Physical
Aijie Liu, Sicong Wang, Hongwei Song, Yawen Liu, Lars Gedda, Katarina Edwards, Leif Hammarstrom, Haining Tian
Summary: In this study, a donor/acceptor (D/A) model system was used to investigate the excited states and charge/energy transfer dynamics in type-II heterojunction polymer dots (Pdots). The results showed that increasing the ratio of ITIC in binary Pdots can slow down charge recombination and enhance photocatalytic hydrogen evolution. Therefore, it is necessary to suppress charge carrier recombination and increase crystallinity and phase segregation in the design of type-II heterojunction Pdots for improved photocatalytic performance.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Pharmacology & Pharmacy
Jonas K. K. Koehler, Lars Gedda, Leonie Wurster, Johannes Schnur, Katarina Edwards, Heiko Heerklotz, Ulrich Massing
Summary: Dual centrifugation (DC) is a new method for preparing different-sized liposomes with controlled structure. The structure of liposomes was investigated using fluorescence spectroscopy and cryo-EM. It was found that a novel type of liposome, small multilamellar vesicles (SMVs), could be produced with highly filled bilayers. Another important finding was the preparation of vesicle-phase transition gradients (VPGs) that can spontaneously close and entrap drugs before application. Lower lipid concentrations during DC can predominantly produce unilamellar vesicles.
Article
Chemistry, Physical
Vahid Forooqi Motlaq, Mattias Ortega-Holmberg, Katarina Edwards, Lars Gedda, Jeppe Lyngso, Jan Skov Pedersen, L. Magnus Bergstrom
Summary: The self-assembly of the anionic bile salt surfactant NaDC and the zwitterionic phospholipid DMPC in physiological saline solution was investigated. The results showed that mixed micelles formed at high content of bile salt, increasing in size as more DMPC was incorporated. At higher mole fractions of DMPC, new structures such as stomatosomes were observed. Bile salt surfactants have enhanced ability to solubilize phospholipid bilayers and form mixed micelles compared to conventional surfactants, which can be rationalized using bending elasticity theory.
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)