Article
Biochemistry & Molecular Biology
Beth A. Griesel, Satoshi Matsuzaki, Albert Batushansky, Timothy M. Griffin, Kenneth M. Humphries, Ann Louise Olson
Summary: This study using 3T3-L1 adipocytes as a model system found that an increase in glucose uptake during the first 3 days of differentiation promoted the expansion of the adipocyte metabolome and proteome. The positive effects were specifically due to increased expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), highlighting its critical role in regulating cellular metabolic remodeling for adipocyte differentiation and maturation.
Article
Biochemistry & Molecular Biology
Francisco J. Cimas, Miguel Angel De la Cruz-Morcillo, Carmen Cifuentes, Natalia Moratalla-Lopez, Gonzalo L. Alonso, Eduardo Nava, Silvia Llorens
Summary: CCT, a saffron-derived apocarotenoid, has anti-adipogenic, anti-inflammatory, and antioxidant activities. This study found that CCT decreased glycerol release and lipid accumulation, inhibited key lipolytic enzymes, and exhibited antioxidant and anti-inflammatory effects. These findings suggest that CCT may be a promising compound for improving lipid mobilisation in obesity.
Article
Chemistry, Multidisciplinary
Yang Yang, Yunyun Wang, Zhijie Zhang, Shifa Wang, Zhen Li
Summary: WSF-CT-11 is a novel AMPK activator that activates AMPK through TRPV1 and has anti-diabetic effects in adipocytes.
Article
Medicine, Research & Experimental
Zeltzin Ceja-Galicia, Diana Calderon-DuPont, Alberto Daniel, Luz Maria Chiu, Andrea Diaz-Villasenor
Summary: This study evaluated the expression of leptin and adiponectin in mature 3T3-L1 adipocytes exposed to palmitate, arsenite, or both. The results showed that the secretion of leptin and adiponectin was decreased, which could result in reduced satiety and decreased insulin sensitivity.
Article
Multidisciplinary Sciences
Laurie B. Cook, Henry D. Ophardt, Rongkun Shen, Bryan H. Pratt, Lucas A. Galbier
Summary: Understanding adipocyte responsiveness to GPCR-derived signals requires consideration of membrane microenvironments, with specific sub-populations of proteins showing significant variation across regions of the cell. Differentiation from pre-adipocytes to adipocytes involves the development of a transient primary cilium, with the MCH receptor 1 translocating to this structure during early adipogenesis. RNA-Seq analysis reveals that MCH signaling is influenced by its receptor localization, impacting the adipogenic transcriptional program and revealing novel signaling pathways related to circadian rhythm, inflammatory response, and ciliary biogenesis in 3T3-L1 cells. These findings suggest potential unintended consequences of using MCH receptor 1 antagonists for clinical interventions on adipose tissue development.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Sina Kavalakatt, Abdelkrim Khadir, Dhanya Madhu, Heikki A. Koistinen, Fahd Al-Mulla, Jaakko Tuomilehto, Jehad Abubaker, Ali Tiss
Summary: UCN3 has shown beneficial effects in adipocytes by improving cellular stress response and glucose uptake, as well as attenuating inflammation and apoptosis.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Hae Rim Jung, Yumi Oh, Dongjun Jang, Seungjae Shin, Soo-Jin Lee, Jiwon Kim, Sang Eun Lee, Jaeik Oh, Giyong Jang, Obin Kwon, Yeonmi Lee, Hui-Young Lee, Sung-Yup Cho
Summary: This study found that the bacteria-derived metabolite PPG can inhibit preadipocyte-to-adipocyte differentiation by suppressing the adiponectin-PPAR pathway. These findings provide a potential candidate for developing anti-obesity drugs.
Article
Toxicology
Linglu Xu, Qianqian Xiao, Chenping Kang, Xuetao Wei, Weidong Hao
Summary: Lanthanum (La), a rare earth element, has various applications in agriculture, industry, and medicine. Studies have suggested that La may affect glycolipid metabolism in the body. In this study, researchers used 3T3-L1 preadipocytes as a cell model to investigate the effects of La(NO3)(3) on adipogenesis and the underlying mechanisms. The results showed that La(NO3)(3) inhibited the adipogenic differentiation of 3T3-L1 preadipocytes, primarily at the early differentiation stage. Furthermore, La(NO3)(3) influenced cell proliferation and regulated signaling pathways involved in adipogenesis.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Guofu Yi, Xia Sang, Yuxia Zhu, Di Zhou, Shuibing Yang, Yue Huo, Yang Liu, Bushra Safdar, Xianyong Bu
Summary: Diabetes mellitus, a metabolic disorder characterized by persistent hyperglycemia, is increasing worldwide. Dietary proteins, particularly soybean peptides (SBPs), contain bioactive peptides with anti-diabetic properties. The peptide SWGEDWGEIW (TSP) from SBPs was found to protect adipocytes from insulin resistance induced by inflammation.
Article
Biology
Birgul Gulnar, Ipek Canatar, Sibel Ozdas
Summary: Obesity causes over 2.8 million deaths annually and is a major risk factor for various diseases, making it crucial to explore alternative treatment options. Recent studies have focused on the potential of natural product-based therapeutics in combating obesity. This study evaluated the antiadipogenic and antiobesogenic effects of pterostilbene, a natural phenolic compound, on 3T3-L1 cells. The results showed that pterostilbene effectively reduced lipid accumulation, increased Glut-4 and Adiponectin levels, and may contribute to weight control.
TURKISH JOURNAL OF BIOLOGY
(2023)
Article
Orthopedics
Jie Zhang, FangLin Ye, AiHua Ye, BaiCheng He
Summary: Lox may reduce the BMP9 osteoblastic potential by inhibiting Wnt/beta-catenin signaling via repressing the expression HIF-1 alpha partially.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Shanshan Zhang, Lingzhe Kong, Ziqiu Jia, Shuli Shao, Lin Pan, Weiyu Wang, Yingning Sun
Summary: Corn peptide (CP), obtained from corn through protease-catalysed hydrolysis, has various health benefits such as antihypertensive, hypoglycemic, antihyperlipidemic, antioxidant, and antitumor effects. It also prevents cardiovascular and cerebrovascular diseases. This study found that CP inhibits lipid droplet accumulation in 3T3-L1 preadipocytes and counteracts high-fat diet-induced obesity in mice.
ARCHIVES OF ANIMAL NUTRITION
(2022)
Article
Medicine, Research & Experimental
Qingxin Fan, Pengjiao Xi, Derun Tian, Lianqun Jia, Yuan Cao, Kaixuan Zhan, Tianwei Sun, Yintong Zhang, Qiming Wang
Summary: The study found that G-Rb1 can dose-dependently promote browning of adipocytes by inhibiting Wnt/beta-catenin signaling. Results showed that G-Rb1 reduced lipid droplet accumulation, increased the expression of genes and proteins associated with brown fat characteristics.
MEDICAL SCIENCE MONITOR
(2021)
Article
Oncology
Li-Mei Zhang, Mo Li, Chen-Chen Tian, Tong-Tong Wang, Shu-Fang Mi
Summary: This study revealed that downregulation of CEBPA in ovarian cancer predicted poor prognosis. Knockout of CEBPA promoted various features of cancer in ovarian cancer cells, while overexpression of CEBPA suppressed these features.
Article
Biochemistry & Molecular Biology
Anna M. Koester, Angeline Geiser, Kamilla M. E. Laidlaw, Silke Morris, Marie F. A. Cutiongco, Laura Stirrat, Nikolaj Gadegaard, Eckhard Boles, Hannah L. Black, Nia J. Bryant, Gwyn W. Gould
Summary: EFR3 and phosphatidylinositol 4-kinase type III?? play crucial roles in regulating insulin-stimulated glucose transport by modulating the distribution of GLUT4 on the plasma membrane, indicating a previously unidentified mechanism in controlling glucose uptake.
BIOSCIENCE REPORTS
(2022)