Article
Oncology
Yongmei Liu, Yanhan Dong, Xinjia He, Anjing Gong, Jinning Gao, Xiaodan Hao, Shuai Wang, Yuqiao Fan, Zibo Wang, Meng Li, Wenhua Xu
Summary: This study identified 10 differentially expressed piRNAs in lung adenocarcinoma (LUAD), among which piR-hsa-211106 was downregulated in LUAD tissues and cell lines. Functional analyses demonstrated that piR-hsa-211106 inhibited LUAD cell proliferation, tumor growth, and migration, while promoting apoptosis, by targeting pyruvate carboxylase (PC) and enhancing chemotherapy sensitivity. These findings suggest that piR-hsa-211106 could serve as a novel diagnostic and therapeutic target for LUAD.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Xianchang Li, Chengbo Li, Shiding Zhang, Chaojun Cui, Jianxin Li, Qianqian Gao
Summary: The study explores the use of Amplex Red (AR) and glucose oxidase to develop a method for detecting glucose, achieving fast detection of glucose concentration through colorimetric and electrochemical methods.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hui Meng, Nannan Yao, Kun Zeng, Nuanfei Zhu, Yue Wang, Biying Zhao, Zhen Zhang
Summary: A novel ratiometric fluorescent immunoassay based on silver nanoparticles was developed for the sensitive determination of dibutyl phthalate. The method showed a low detection limit and a wide linear range, and it improved the environmental correction and precision.
Article
Chemistry, Multidisciplinary
Yang Li, Yingying Zhou, Danyan Luo, Zhaoyi Yang, Lillian Ruoduo Hu, Zhen Huang
Summary: This study reports the first synthesis of novel 2-Se-thymidine triphosphate (TTP)-T-Se, utilizing a single-selenium atom-specific modification strategy (SAM) against T/G mismatches to enhance specificity and sensitivity in DNA polymerization and detection. SAM significantly suppresses non-specific products and enhances DNA synthesis specificity by approximately 10,000 fold, allowing for the detection of clinical COVID-19 viral RNA in low copy numbers.
CHEMICAL COMMUNICATIONS
(2021)
Article
Cardiac & Cardiovascular Systems
Prakash Doddapattar, Rishabh Dev, Madankumar Ghatge, Rakesh B. Patel, Manish Jain, Nirav Dhanesha, Steven R. Lentz, Anil K. Chauhan
Summary: This study demonstrates that myeloid cell-specific PKM2 regulates macrophage efferocytosis and atherosclerosis. Deletion or inhibition of PKM2 reduces atherosclerotic lesions, suppresses inflammation, and enhances macrophage efferocytosis.
CIRCULATION RESEARCH
(2022)
Article
Chemistry, Physical
Shameema Rana, Jaibir Kherb
Summary: This study investigated the effect of anions on the fluorescence of carbon quantum dots with amide groups. Chaotropic lipophilic anions caused fluorescence quenching, while kosmotropic hydrophilic anions had little impact on the nanomaterial fluorescence. The functional groups on macromolecules and the thermodynamic parameters of anions in a solvent play a key role in the observed anion specificity.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Multidisciplinary
Dhanapal Jothi, Sathishkumar Munusamy, Sathish Sawminathan, Sathiyanarayanan Kulathu Iyer
Summary: A naphthalimide based Schiff base probe was designed and synthesized for specific detection of Fe3+, showing low detection limit and fast response time. The probe exhibited significant optical property changes in the presence of Fe3+, with potential lysosome targetability.
Article
Biochemistry & Molecular Biology
Christine Contini, Julia Kuntz, Ulrich Massing, Irmgard Merfort, Karl Winkler, Gerhard Puetz
Summary: We investigated the effects of different lipids on the activity of the angiotensin II type 1 receptor (AT1R). The results showed that cells preincubated with Very low-density lipoprotein (VLDL) exhibited reduced calcium release triggered by angiotensin II, but further analysis revealed that this observation might be an artifact. Incubation with VLDL also decreased intracellular fura-2 levels and the maximal obtainable ratio of calcium ions in cells. These findings questioned the initial results and emphasized the importance of careful interpretation in fura-2 measurements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Junghyun Ryu, Youngmi Kim
Summary: This study reports a BODIPY-metal complex as a colorimetric and fluorimetric indicator for the rapid detection of GHB. The complex can be used for qualitative and quantitative analysis in a broad range of beverages.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Spectroscopy
Subrata Mondal, Priti Gupta, Fazlur Rahaman, Prabhat Gautam, I. C. Lekshmi
Summary: Thiazole based receptor was designed and synthesized for colorimetric and fluorimetric detection of fluoride ion. The receptor showed significant absorption and fluorescence changes in the presence of fluoride ions, with high detection sensitivity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Aikaterini G. Karra, Ioannis Tsialtas, Foteini D. Kalousi, Achilleas Georgantopoulos, Evangelia Sereti, Konstantinos Dimas, Anna-Maria G. Psarra
Summary: Mitochondria are crucial for cellular physiology as they generate energy and regulate biological functions. Dysregulation of mitochondrial function is associated with various pathologies, including cancer. The mitochondrial glucocorticoid receptor (mtGR) plays a role in regulating mitochondrial transcription, metabolism, energy production, apoptosis, and oxidative stress. The interaction between mtGR and pyruvate dehydrogenase (PDH), a key player in cancer metabolism, suggests the involvement of mtGR in cancer development. In this study, using a xenograft mouse model, overexpression of mtGR in hepatocarcinoma cells was found to promote tumor growth, reduce OXPHOS biosynthesis and PDH activity, and alter glucose metabolism, similar to the Warburg effect. Autophagy activation in mtGR-associated tumors further supports tumor progression. Increased mitochondrial localization of mtGR is suggested to contribute to tumor progression via mtGR/PDH interaction, suppressing PDH activity and modulating mtGR-induced mitochondrial transcription, favoring glycolytic energy production in cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: In this study, an automated flow method based on zone fluidics was developed for the simultaneous determination of glutathione (GSH) and glutathione disulfide (GSSG). The method showed good selectivity, high accuracy, and performed well in analyzing real yeast samples.
Article
Chemistry, Multidisciplinary
Dhanamjai Penta, Pratima Tripathi, Dheeran Rajarajan, Jagadish Natesh, Priya Mondal, Syed Musthapa Meeran
Summary: This study investigated the potential therapeutic interventions of DIM in TNBC and its metabolic reprogramming in enhancing the efficacy of CC. The results showed that DIM induced metabolic catastrophe in TNBC cells by regulating aerobic glycolysis and intermediate metabolism. The combination of DIM and CC significantly inhibited TNBC tumor growth and downregulated key aerobic glycolysis mediators. These findings suggest that DIM can be developed as an adjuvant therapy for treating metabolically dysregulated TNBC.
Article
Biochemical Research Methods
Jose Yeste, Marc Azagra, Maria A. Ortega, Alejandro Portela, Gergo Matajsz, Alba Herrero-Gomez, Yaewon Kim, Renuka Sriram, John Kurhanewicz, Daniel B. Vigneron, Irene Marco-Rius
Summary: We developed a microfluidic device compatible with hyperpolarization-enhanced MR spectroscopic imaging. It measures up to 8 chemical reactions simultaneously with a single hyperpolarized sample. This technology allows for real-time, in situ analysis of chemical reactions and provides a new high-throughput method for chemical research and biological metabolic analysis.
News Item
Optics
David Baddeley
Summary: The introduction of a two-step deconvolution workflow improves the detection of fluorescence in fluctuation-based super-resolution imaging, allowing for the capture of a square millimeter field of view in as little as ten minutes.
Editorial Material
Obstetrics & Gynecology
Piya Chaemsaithong, Roberto Romero, Waranyu Lertrut, Arunee Singsaneh, Pisut Pongchaikul
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Article
Obstetrics & Gynecology
Dahiana M. Gallo, Roberto Romero, Mariachiara Bosco, Tinnakorn Chaiworapongsa, Nardhy Gomez-Lopez, Marcia Arenas-Hernandez, Eunjung Jung, Manaphat Suksai, Francesca Gotsch, Offer Erez, Adi L. Tarca
Summary: The study found that pregnant women with severe PPH had higher median maternal plasma concentrations of IL-16, IL-6, IL-12/IL-23p40, MCP-1, and IL-1 beta than patients without PPH. These cytokines could serve as biomarkers or therapeutic targets.
JOURNAL OF PERINATAL MEDICINE
(2023)
Article
Obstetrics & Gynecology
Doron Kabiri, Dror Raif Nesher, Danielle Luxenbourg, Amihai Rottenstreich, Joshua Rosenbloom, Yosef Ezra, Simcha Yagel, Shay Porat, Roberto Romero
Summary: Vaginal progesterone treatment in women with threatened preterm labor and a short cervix after 24 weeks of pregnancy is associated with lower rates of preterm birth. The treatment also prolongs the time interval from diagnosis to delivery and reduces the frequency of neonatal intensive care unit admission.
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS
(2023)
Article
Obstetrics & Gynecology
Maria Teresa Gervasi, Roberto Romero, Elisa Cainelli, Paola Veronese, Maria Rosa Tran, Eunjung Jung, Manaphat Suksai, Mariachiara Bosco, Francesca Gotsch
Summary: This study suggests that asymptomatic intra-amniotic inflammation in the mid-trimester of pregnancy can increase the risk of neurodevelopmental disorders in children. Early recognition and treatment of maternal immune activation may be a strategy for preventing such disorders in offspring.
JOURNAL OF PERINATAL MEDICINE
(2023)
Article
Obstetrics & Gynecology
Nandor Gabor Than, Roberto Romero, Daniel Gyorffy, Mate Posta, Gaurav Bhatti, Bogdan Done, Piya Chaemsaithong, Eunjung Jung, Manaphat Suksai, Francesca Gotsch, Dahiana M. Gallo, Mariachiara Bosco, Bomi Kim, Yeon Mee Kim, Tinnakorn Chaiworapongsa, Simona W. Rossi, Andras Szilagyi, Offer Erez, Adi L. Tarca, Zoltan Papp
Summary: This study identified different molecular subclasses of preeclampsia through longitudinal proteomic profiling, laying the foundation for the development of new diagnostic and personalized tools for prevention.
JOURNAL OF PERINATAL MEDICINE
(2023)
Letter
Obstetrics & Gynecology
Bo Hyun Yoon, Roberto Romero, Kyung Joon Oh, Hyeon Ji Kim, Eunjung Jung, Francesca Gotsch
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Article
Obstetrics & Gynecology
Roberto Romero
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Article
Obstetrics & Gynecology
Tomi Kanninen, Eunjung Jung, Dahiana M. Gallo, Ramiro Diaz-Primera, Roberto Romero, Francesca Gotsch, Manaphat Suksai, Mariachiara Bosco, Tinnakorn Chaiworapongsa
Summary: Preeclampsia and fetal growth disorders are pregnancy-specific conditions that share common pathophysiological mechanisms. This study suggests that the severity of the maternal inflammatory response is highest in patients with preeclampsia, while patients with an SGA fetus also show elevated inflammatory response.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2023)
Review
Obstetrics & Gynecology
Agustin Conde-Agudelo, Roberto Romero
Summary: This review examines the efficacy of vaginal progesterone (VP) in preventing preterm birth (PTB) in different subsets of high-risk women. The evidence suggests that VP is effective in reducing the risk of PTB and improving perinatal outcomes in certain groups, such as singleton gestations with a short cervix. However, further research is needed to determine its efficacy in other high-risk groups.
JOURNAL OF PERINATAL MEDICINE
(2023)
Review
Obstetrics & Gynecology
Dahiana M. Gallo, Roberto Romero, Mariachiara Bosco, Francesca Gotsch, Sunil Jaiman, Eunjung Jung, Manaphat Suksai, Carlos Lopez Ramon y Cajal, Bo Hyun Yoon, Tinnakorn Chaiworapongsa
Summary: Green-stained amniotic fluid, also known as meconium-stained amniotic fluid, is a common obstetric hazard. It can be caused by the passage of fetal colonic content or intraamniotic bleeding. Birth with green-stained amniotic fluid is associated with fetal acidemia and potential complications like respiratory distress and seizures. Infection/inflammation inside the amniotic fluid plays a role in the development of meconium-stained amniotic fluid and can lead to clinical chorioamnionitis and neonatal sepsis.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Review
Obstetrics & Gynecology
Agustin Conde-Agudelo, Roberto Romero, Anoop Rehal, Maria L. Brizot, Vicente Serra, Eduardo Da Fonseca, Elcin Cetingoz, Argyro Syngelaki, Alfredo Perales, Sonia S. Hassan, Kypros H. Nicolaides
Summary: This study evaluated the efficacy of vaginal progesterone for preventing preterm birth and adverse perinatal outcomes in twin pregnancies. The results showed that vaginal progesterone did not prevent preterm birth or improve perinatal outcomes in unselected twin pregnancies. However, it appeared to reduce the risk of preterm birth and neonatal morbidity and mortality in twin pregnancies with a sonographic short cervix.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Review
Microbiology
Jonathan J. Panzer, Roberto Romero, Jonathan M. Greenberg, Andrew D. Winters, Jose Galaz, Nardhy Gomez-Lopez, Kevin R. Theis
Summary: The existence of a microbiota in the human placenta is still debated. Recent DNA sequencing investigations reported the presence of a microbiota in typical human term placentas, but it could be background DNA or contamination. Re-analysis of publicly available 16S rRNA gene datasets showed that Lactobacillus, a vaginal bacterium, was highly abundant in placentas but disappeared after applying DNA contaminant removal. Furthermore, bacterial profiles of placental samples clustered primarily by study origin and mode of delivery, suggesting that placentas delivered at term are unlikely to be the original source of observed bacterial DNA signals.
Letter
Obstetrics & Gynecology
Piya Chaemsaithong, Piroon Jenjaroenpun, Pisut Pongchaikul, Arunee Singsaneh, Iyarit Thaipisuttikul, Roberto Romero, Thidathip Wongsurawat
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Article
Obstetrics & Gynecology
Priya Prasad, Roberto Romero, Tinnakorn Chaiworapongsa, Nardhy Gomez-Lopez, Anderson Lo, Jose Galaz, Andreea B. B. Taran, Eunjung Jung, Francesca Gotsch, Nandor Gabor Than, Adi L. L. Tarca
Summary: Approximately 47% of women with preterm labor deliver at term, but their infants are at higher risk for being small for gestational age and having neurodevelopmental disorders. This study tested the involvement of components of the insulin-like growth factor system in preterm labor and found higher levels of certain components in women with a history of preterm labor.
FETAL DIAGNOSIS AND THERAPY
(2023)
Article
Medicine, Research & Experimental
Nardhy Gomez-Lopez, Roberto Romero, Maria Fernanda Escobar, Javier Andres Carvajal, Maria Paula Echavarria, Ludwig L. Albornoz, Daniela Nasner, Derek Miller, Dahiana M. Gallo, Jose Galaz, Marcia Arenas-Hernandez, Gaurav Bhatti, Bogdan Done, Maria Andrea Zambrano, Isabella Ramos, Paula Andrea Fernandez, Leandro Posada, Tinnakorn Chaiworapongsa, Eunjung Jung, Valeria Garcia-Flores, Manaphat Suksai, Francesca Gotsch, Mariachiara Bosco, Nandor Gabor Than, Adi L. Tarca
Summary: In this study, the plasma proteome of pregnant and non-pregnant COVID-19 patients and controls was characterized. The findings revealed shared and pregnancy-specific proteomic changes in COVID-19 patients compared to controls, and the plasma proteome accurately identifies COVID-19 patients, even when they are asymptomatic. This study provides insight into the pathogenesis of COVID-19 and explains the more severe outcomes observed in pregnant women.
COMMUNICATIONS MEDICINE
(2023)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
