Journal
ANALYTICAL BIOCHEMISTRY
Volume 397, Issue 1, Pages 115-117Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.09.023
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A bead-based assay was developed for highly sensitive single molecule DNA detection. Rolling circle amplification (RCA), an isothermal amplification technique that creates tandem repeated sequences, Was used in combination with a fluorescent complementary DNA to create dense Clusters of fluorescence. These Clusters, each corresponding to a single target molecule, can be detected unambiguously due to their high signal/noise ratios. The limit of detection of this assay is approximately 1 amol. This simple single molecule assay allows high detection sensitivity Without the use of complex equipment. (C) 2009 Elsevier Inc. All rights reserved.
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