Article
Biology
Akil Akhtar, Madhu Lata, Sonali Sunsunwal, Amit Yadav, Kajal Lnu, Srikrishna Subramanian, T. N. C. Ramya
Summary: Uncultured microbes are a valuable source of novel genes and gene products. Functional metagenomics can be used to identify and annotate these novel gene products. In this study, a metagenomic phage display library was constructed and screened to identify previously unannotated carbohydrate binding domains. Several new carbohydrate binding domains were discovered, including a levan binding domain and four complex N-glycan binding domains.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Imtiaj Hasan, Sharif Nasibur Rahman, Md Mahenur Islam, Sourov Kumar Ghosh, Mizanur Rahman Mamun, Md Belal Uddin, Ranajit Kumar Shaha, Syed Rashel Kabir
Summary: AGL, a 15-kDa lectin isolated from Amaranthus gangeticus seeds, exhibited various biological activities including antibacterial, antitumor, and modulation of apoptosis-related gene expression.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Mohamad Chebbo, Said Assou, Veronique Pantesco, Catherine Duez, Marie C. Alessi, Pascal Chanez, Delphine Gras
Summary: Platelet transcriptome analysis is important for identifying disease-specific molecular signatures, but contamination during platelet purification process can greatly alter the transcriptome results, emphasizing the need for strict precautions to avoid contamination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Manreet Kaur, Vanessa Yardley, Ke Wang, Jinit Masania, Adolfo Botana, Randolph R. J. Arroo, Mingzhong Li
Summary: The study aims to explore the formulation of ART cocrystals as oral dosage forms to enhance bioavailability, with results showing significant increase in solubility. PVP-VA was identified as the most effective crystallization inhibitor among a set of polymers, providing crucial information for formulation development. Experiments on the dissolution performance and bioavailability of ART cocrystals with excipients offered valuable insights into their effects.
MOLECULAR PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Mabroka H. Saad, Nagwa M. Sidkey, Rizwan Hasan Khan, Esmail M. El-Fakharany
Summary: In this study, two types of lectins were purified from Nostoc muscorum and characterized for their physical properties and antiviral activity. Nostoc muscorum showed potential as a unique source for antiviral drug discovery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Xiaona Li, Honggang Liu, Changtai Zhao, Jung Tae Kim, Jiamin Fu, Xiaoge Hao, Weihan Li, Ruying Li, Ning Chen, Duanyun Cao, Zhenwei Wu, Yuefeng Su, Jianwen Liang, Xueliang Sun
Summary: Inorganic solid-state electrolytes (SSEs) have potential use in high-energy solid-state batteries, but the mechanism of fast ion conduction in SSEs is not well understood. Through a combined analysis approach, this study identifies critical parameters that influence ion conductivity in SSEs and verifies them in the xLiCl-InCl3 system. The findings show that the concentration of mobile carriers and the hopping rate both have independent influences on the ionic conductivity. Temperature change affects the hopping rate and ionic conductivity, while migration entropy plays a significant role in fast Li+ migration. The study highlights the multiple dependent variables responsible for ionic conduction behavior in SSEs.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Roopashri N. Arekal, J. Savitha, Ashwini Kumar, B. S. Gunashree, M. S. Divyashree
Summary: A freshwater green microalgal strain was isolated and a lectin with strong hemagglutination activity was identified. The lectin was purified using an affinity chromatographic technique and characterized. The study revealed that the lectin has similar characteristics to a hemagglutinin, indicating its potential for various biological purposes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Multidisciplinary
Andrey V. Shibaev, Alexander S. Ospennikov, Elizaveta K. Kuznetsova, Alexander I. Kuklin, Teimur M. Aliev, Valentin V. Novikov, Olga E. Philippova
Summary: This study investigates the effects of solubilizing hydrophobic and hydrophilic substances on the rheological properties and structure of wormlike surfactant micelles. The findings reveal that hydrophobic additives are completely solubilized inside the micelles, while only a small fraction of hydrophilic additives penetrate the micelles. Despite the differences in localization, both types of additives induce the breaking of micelles, resulting in a drop in viscosity.
Article
Chemistry, Analytical
Wolf Hiller, Bastian Grabe
Summary: This article discusses a universal calibration method for the determination of polymer molar mass dependence using diffusion-ordered spectroscopy (DOSY) and compares it to size exclusion chromatography (SEC) and other DOSY methods. By implementing the Mark-Houwink approach, a single mathematical function is derived that describes both structure- and solvent-independent calibrations for DOSY. Through extensive testing on different polymers and solvents, it is shown that this method can accurately determine molar masses and new Mark-Houwink parameters.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Baris Ozel, Danuta Kruk, Milosz Wojciechowski, Maciej Osuch, Mecit Halil Oztop
Summary: In this study, whey protein isolate-based composite hydrogels with encapsulated black carrot extract were prepared through heat-induced gelation. The use of gum tragacanth, pectin, and xanthan gum polysaccharides in the hydrogels was found to modulate their properties. H-1 spin-lattice relaxation experiments were conducted to analyze the influence of different polysaccharides and black carrot on the dynamic properties of water molecules in the hydrogel network, revealing highly anisotropic translation diffusion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Arthur Lenoch, Marcel Schumacher, Andre H. Groeschel, Cornelia Cramer, Monika Schoenhoff
Summary: Self-diffusion of poly(acrylic acid) (PAA) in dilute solution is studied using pulsed field gradient (PFG)-NMR, and its correlation with molecular weight and pH are analyzed. The diffusion measurements offer an alternative method to estimate chain length properties of PAA with minimal sample preparation. The results indicate that pH significantly affects the conformation of PAA chains, even for short chains.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2023)
Article
Biochemical Research Methods
Lori Manoukian, Jose A. Correa, Robin S. Stein, Dominic Frigon, Sidney Omelon
Summary: Inorganic polyphosphates (polyPs) are present in both eukaryotic and prokaryotic cells. This study optimized extraction methods for the identification and measurement of polyPs. The results showed that extraction processes reduced polyP migration and dispersion, leading to a potential overestimation of synthetic polyP chain length.
Article
Agriculture, Multidisciplinary
Wannes L. De Man, Ewoud Vaneeckhaute, Niels De Brier, Arno G. B. Wouters, Johan A. Martens, Eric Breynaert, Jan A. Delcour
Summary: The structural heterogeneity of water-extractable arabinoxylan (WE-AX) impacts wheat flour functionality, as revealed by H-1 diffusion-ordered (DOSY) nuclear magnetic resonance (NMR) spectroscopy. Different subpopulations within WE-AX fractions have varying diffusion rates, with high self-diffusivity subpopulations containing high levels of monosubstituted xylose and low self-diffusivity subpopulations rich in disubstituted xylose.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Jun Heo, Chang Woo Kwon, Juno Lee, Haena Park, Hyunjong Yu, Pahn-Shick Chang
Summary: A novel lipase was discovered from Korean chestnut, showing high purification fold, specific activity against different triglycerides, and maximal activity at alkaline pH. The enzyme exhibited high affinity for its substrate and could be activated by Mg2+ and Ca2+. Its molecular structure was similar to a lectin in the biomedical field.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Article
Engineering, Chemical
Amina Othmani, Juliana John, Harishkumar Rajendran, Abdeldjallil Mansouri, Mika Sillanpaa, Padmanaban Velayudhaperumal Chellam
Summary: This paper provides critical insight on various lignocellulosic fibers used for colored and wastewater decontamination, discussing their potential as cost-effective and eco-friendly alternatives. It also outlines mathematical modeling describing the kinetics, isotherms, type of adsorption, and influential factors in the adsorption process, along with discussing new trends for valorizing vegetable materials in wastewaters decontamination.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Francisco Javier Canada, Angeles Canales, Pablo Valverde, Beatriz Fernandez de Toro, Monica Martinez-Orts, Paola Oquist Phillips, Amaia Pereda
Summary: Carbohydrates, either as free molecules or conjugated with other biomolecules, play important roles in various biological processes. Nuclear Magnetic Resonance spectroscopy (NMR) is a versatile tool for studying the structures and interactions of carbohydrates, providing information about their sequences, structures, and local geometries. Labeling carbohydrates with 13C enhances the resolution and detail of the analyzed structures. Moreover, combining NMR with molecular modeling and theoretical calculations offers insights into the conformational flexibility of carbohydrates. Additionally, the use of partially oriented media or paramagnetic perturbations allows for the study of longer and branched glycan chains. This review presents examples and an overview of recent and relevant NMR applications in the field of glycobiology.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Reyes Nunez-Franco, Gonzalo Jimenez-Oses, Jesus Jimenez-Barbero, Francisca Cabrera-Escribano, Antonio Franconetti
Summary: A multidisciplinary study is conducted to investigate the pi-pi interactions established by pyrylium frameworks as pi-hole donors. The combination of CSD analysis, computational modelling, and experimental NMR spectroscopy data provides essential information on the key parameters characterizing these interactions, paving the way for further applications of this versatile heterocycle.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Francesco Milanesi, Luca Unione, Ana Arda, Cristina Nativi, Jesus Jimenez-Barbero, Stefano Roelens, Oscar Francesconi
Summary: Glycomics research has revealed the essential role of carbohydrates in biological systems and the importance of chemical tools in studying glycan function. While biomimetic receptors can selectively bind to simple saccharides, this study demonstrates the unprecedented recognition of a complex glycopeptide by a biomimetic receptor. The receptor shows high affinity for the core disaccharide of the N-glycan and has the potential for versatile applications in glycoscience.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Marta G. Lete, Antonio Franconetti, Sara Bertuzzi, Sandra Delgado, Mikel Azkargorta, Felix Elortza, Oscar Millet, Gonzalo Jimenez-Oses, Ana Arda, Jesus Jimenez-Barbero
Summary: Fluorine (F-19) incorporation into lectins has allowed monitoring of carbohydrate binding through NMR spectroscopy. Galectin-3 (Gal3) and Galectin-8 (Gal8) with one and two carbohydrate recognition domains (CRDs), respectively, were chosen. The presence of fluorine did not significantly affect glycan binding affinity, and allowed differentiation of binding events at different sites within the same lectin.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Ana Pozo-Rodriguez, Juan A. A. Mendez-Liter, Rocio Garcia-Villalba, David Beltran, Eva Calvino, Andres G. Santana, Laura I. I. De Eugenio, Francisco Javier Canada, Alicia Prieto, Jorge Barriuso, Francisco A. A. Tomas-Barberan, Maria Jesus Martinez
Summary: Resveratrol was glycosylated by using rXynSOS-E236G glycosynthase, resulting in the production of 3-O-beta-D-xylobiosyl resveratrol as the major product. Response surface methodology was used to optimize the reaction, yielding 35% of 3-O-beta-D-xylobiosyl resveratrol. Xylobiosylation decreased the antioxidant capacity of resveratrol, but significantly improved its solubility for improved delivery and transit to the colon.
Article
Nanoscience & Nanotechnology
Anna Blasi-Romero, Molly Angstrom, Antonio Franconetti, Taj Muhammad, Jesus Jimenez-Barbero, Ulf Goransson, Carlos Palo-Nieto, Natalia Ferraz
Summary: This study combines the wound-healing properties of KR-12 peptide with wood-derived cellulose nanofibrils (CNFs) to create bioactive materials for treating chronic wounds. Different coupling chemistries were used to covalently attach KR-12 derivatives onto CNFs, and the bioactivity of KR12-CNF conjugates was evaluated in terms of antibacterial activities and anti-inflammatory effects. The results showed that thiol-ene chemistry produced the most bioactive conjugates, attributed to favorable peptide conformation and accessibility. This study advances the development of CNF-based materials for chronic wound care and provides insights into the effect of conjugation chemistry on the bioactivity of host defense peptides.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Zhen Wang, Ana Poveda, Qingju Zhang, Luca Unione, Herman S. Overkleeft, Gijsbert A. van der Marel, Jimenez-Barbero Jesus, Jeroen D. C. Codee
Summary: Researchers have successfully synthesized a series of polysaccharide fragments with a unique structure that can activate T cells and bind to specific receptors. Detailed structural studies have revealed the secondary structure of these fragments, providing a basis for further understanding their mode of action.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Organic
Matteo A. . Tallarida, Fabrizio Olivito, Claudio D. Navo, Vincenzo Algieri, Antonio Jiritano, Paola Costanzo, Ana Poveda, Maria J. Moure, Jesus Jimenez-Barbero, Loredana Maiuolo, Gonzalo Jimenez-Oses, Antonio De Nino
Summary: The synthesis of polysubstituted spirocyclopropyl oxindoles using rare-earth metal (REM) salts, particularly Sc(OTf)3, was achieved with high diastereoselectivity (<= 94:6:0:0) in a multicomponent reaction. Density functional theory calculations on the model reaction confirmed the observed selectivity and demonstrated that the coordinating capabilities and oxophilicity of the metal played a crucial role in inducing the formation of one main diastereoisomer.
Article
Biochemistry & Molecular Biology
Hans-Christian Siebert, Thomas Eckert, Anirban Bhunia, Nele Klatte, Marzieh Mohri, Simone Siebert, Anna Kozarova, John W. Hudson, Ruiyan Zhang, Ning Zhang, Lan Li, Konstantinos Gousias, Dimitrios Kanakis, Mingdi Yan, Jesus Jimenez-Barbero, Tibor Kozar, Nikolay E. Nifantiev, Christian Vollmer, Timo Brandenburger, Detlef Kindgen-Milles, Thomas Haak, Athanasios K. Petridis
Summary: The global outbreak of SARS-CoV-2/COVID-19 has provided an opportunity and challenge to accumulate a large amount of biomedical data and test new concepts and strategies to combat the pandemic. This study correlated clinical biomedical data with structural biology and biophysical data to evaluate new diagnostic and therapeutic approaches against SARS-CoV-2. The methods used were effective in identifying potential peptide sequences and carbohydrate moieties to protect the blood-brain barrier and develop new therapeutic approaches against long-COVID symptoms.
Article
Chemistry, Multidisciplinary
Angeles Canales, Javier Sastre, Jose M. Orduna, Cindy M. Spruit, Javier Perez-Castells, Gema Dominguez, Kim M. Bouwman, Roosmarijn van der Woude, Francisco Javier Canada, Corwin M. Nycholat, James C. Paulson, Geert-Jan Boons, Jesus Jimenez-Barbero, Robert P. de Vries
Summary: Influenza virus infection remains a threat to human health due to the drifting of viral hemagglutinins, which evade infection and vaccine-induced antibody responses. Recent H3N2 and pandemic H1 viruses specifically recognize glycan structures containing at least three N-acetyllactosamine units. This study characterizes the glycan specificity of H1 variants, including the one responsible for the 2009 pandemic outbreak, and investigates the preference for tri-LacNAc motifs in human-type receptor-adapted viruses using glycan arrays, tissue binding analyses, and nuclear magnetic resonance experiments.
Article
Chemistry, Multidisciplinary
Giulio Fittolani, Theodore Tyrikos-Ergas, Ana Poveda, Yang Yu, Nishu Yadav, Peter H. Seeberger, Jesus Jimenez-Barbero, Martina Delbianco
Summary: This article introduces a synthetic glycan molecule that adopts a stable secondary structure not found in nature, called a glycan hairpin. The synthetic glycan is created by combining natural glycan motifs and utilizing unconventional hydrogen bonds and hydrophobic interactions. Nuclear magnetic resonance conformational analysis and long-range inter-residue nuclear Overhauser effects confirm the folded conformation of the synthetic glycan hairpin. The ability to control the 3D shape of glycans has the potential to provide more foldamer scaffolds with programmable properties and functions.
Review
Chemistry, Multidisciplinary
Jon I. Quintana, Unai Atxabal, Luca Unione, Ana Arda, Jesus Jimenez-Barbero
Summary: Nuclear Magnetic Resonance (NMR) is widely used to study glycan-protein molecular recognition events, providing qualitative and quantitative information at different levels of resolution and complexity. However, the weak affinity of lectin-sugar interactions is overcome by multivalency in biological processes. The application of NMR methods to monitor multivalent lectin-glycan interactions is challenging due to the disappearance of NMR signals when large macromolecular complexes are formed.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Carlo Pifferi, Leire Aguinagalde, Ane Ruiz-de-Angulo, Nagore Sacristan, Priscila Tonon Baschirotto, Ana Poveda, Jesus Jimenez-Barbero, Juan Anguita, Alberto Fernandez-Tejada
Summary: The overexpression of aberrantly glycosylated tumor-associated mucin-1 (TA-MUC1) in human cancers makes it a major target for the development of anticancer vaccines derived from synthetic MUC1-(glyco)peptide antigens. However, glycopeptide-based subunit vaccines are weakly immunogenic, requiring adjuvants and/or additional immunopotentiating approaches to generate optimal immune responses.
Article
Immunology
Leticia Martin-Cruz, Marcos Vinuela, Ioanna Kalograiaki, Alba Angelina, Paola Oquist-Phillips, Irene Real-Arevalo, Francisco Javier Canada, Jose Ignacio Tudela, Luis Molto, Jesus Moreno-Sierra, Jose Luis Subiza, Oscar Palomares
Summary: This study reveals novel molecular mechanisms by which heparan sulfate-related structures associated with tumor cells promote the generation of functional Tregs in cancer. Furthermore, the presence of a circulating human Ca10 counterpart (Ca10H) in cancer patients and its correlation with tumor size and metastasis suggests important clinical implications in cancer treatment.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ana Pozo-Rodriguez, Juan A. Mendez-Liter, Laura I. de Eugenio, Manuel Nieto-Dominguez, Eva Calvino, Francisco Javier Canada, Andres G. Santana, Jaime Diez, Juan L. Asensio, Jorge Barriuso, Alicia Prieto, Maria Jesus Martinez
Summary: The study explores the use of endoxylanases and their glycosynthase variants in the valorization of hemicellulosic residues and the synthesis of glycosides with improved properties. The results demonstrate the high catalytic efficiency and substrate versatility of these enzymes, particularly in the degradation of beechwood xylan and wheat arabinoxylan. The glycosynthase variant shows enhanced capacity in the synthesis of xylooligosaccharides and glycosylation of phenolic compounds with antioxidant properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)