Article
Chemistry, Analytical
Jiali Zhang, Zhuo Yang, Liying Liu, Tinglu Zhang, Lilei Hu, Chunrui Hu, Hu Chen, Ruihua Ding, Bo Liu, Chang Chen
Summary: This paper introduces an ultrafast polymerase chain reaction (PCR) equipment designed for virus detection. The equipment consists of a silicon-based PCR chip, a thermocycling module, an optical detection module, and a control module. It has the advantages of rapid and accurate detection efficiency, making it suitable for large-scale epidemic prevention and control.
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Biochemical Research Methods
Ziqing Yu, Lei Xu, Weiyuan Lyu, Feng Shen
Summary: Digital biological analysis compartmentalizes targets of interest and performs detection and investigation. This study presents a parallel multistep digital analysis SlipChip, which enables the manipulation of a large number of droplets for digital biological analysis. The SlipChip utilizes a self-partitioning droplet formation mechanism and can perform digital loop-mediated isothermal amplification and digital CRISPR detection in parallel.
Article
Biochemical Research Methods
Jinrong Shen, Jihong Zheng, Zhenqing Li, Yourong Liu, Fengxiang Jing, Xinjun Wan, Yoshinori Yamaguchi, Songlin Zhuang
Summary: The study introduces an effective technique for the absolute quantification of target nucleic acids, with self-developed software controlling functional units to achieve high-resolution one-time gene chip imaging, speeding up the lab-to-application process.
Article
Biochemical Research Methods
Yen-Ling Fang, Chih-Hung Wang, Yi-Sin Chen, Chun-Chih Chien, Feng-Chih Kuo, Huey-Ling You, Mel S. Lee, Gwo-Bin Lee
Summary: The study developed an integrated microfluidic chip for rapid isolation of bacteria from blood, aiding in early sepsis diagnosis. The device is efficient with a high capture rate of 85% and the entire detection process can be completed in just 4 hours.
Article
Multidisciplinary Sciences
Abbas Jalili, Maryam Bagheri, Amir Shamloo, Amir Hossein Kazemipour Ashkezari
Summary: A rapid droplet-based photonic PCR system was developed using a gold nanofilm-based microfluidic chip and a plasmonic photothermal cycler, achieving fast thermal cycling and successful amplification of the AOX gene. The microfluidic chip demonstrated great performance in droplet-based PCR.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Ravil R. Garafutdinov, Dmitry A. Chemeris, Assol R. Sakhabutdinova, Konstantin Moiseev, Said F. Urmancheev, Constantin Mikhaylenko, Lavrentyi Yu Privalov, Alexey Chemeris
Summary: Polymerase chain reaction (PCR) is widely used for nucleic acids amplification. This study reports the use of convective PCR (cPCR) in standard microtubes for efficient amplification of nucleic acids. By optimizing reaction conditions, high sensitivity detection and accumulation of amplification products were achieved, enabling reliable detection of SARS-CoV-2 coronavirus RNA from COVID-19 patients' nasopharyngeal swabs.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yujun Xing, Jie Liang, Fei Dong, Jirong Wu, Jianrong Shi, Jianhong Xu, Jinke Wang
Summary: We developed a novel loop-mediated isothermal amplification (LAMP) method using DNA captured on polyacrylamide microparticles (PAMMPs) as templates (PAMMPs@DNA-LAMP) for rapid qualitative detection of genetically modified organisms (GMOs). The PAMMPs@DNA-LAMP method allowed for rapid DNA extraction (5-10 min) and amplification (around 30 min) at a constant temperature of 63°C. The DNA captured by PAMMPs could be effectively detected by both conventional and quantitative PCR (qPCR) and LAMP.
Article
Chemistry, Multidisciplinary
Kangning Wang, Qingran Wang, Canfu Peng, Yu Guo, Yan Li, Jia Zhou, Wenming Wu
Summary: This study proposes a new PCR method based on rapid PCR chip optimization using a liquid metal bath, which achieves precise and rapid temperature control. The results show that the system can be successfully used in PCR devices, and the optimized PCR chip further improves temperature response speed.
Article
Multidisciplinary Sciences
Cyrille L. Delley, Adam R. Abate
Summary: In this study, a modular framework is described to simplify the generation of multifunctional beads and allow for easy extension to new targets. The current barcode designs are limited to specific applications, but with this new approach, repurposing to novel targets becomes more efficient.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Zhenqing Li, Yifei Wang, Zehang Gao, Shinichi Sekine, Qingxiang You, Songlin Zhuang, Dawei Zhang, Shilun Feng, Yoshinori Yamaguchi
Summary: We proposed a double-layer droplet CF-PCR microfluidic chip to overcome the drawbacks of CF-PCR, such as high resistance and evaporation. Experimental results showed that the target genes of Porphyromonas gingivalis were successfully amplified within a short period of time, providing a new approach for rapid bacterial detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Engineering, Biomedical
Viviane Klassen de Oliveira, Bruno Dias Camargo, Fabiana Alexandrino, Luis Gustavo Morello, Fabricio Klerynton Marchini, Mateus Nobrega Aoki, Lucas Blanes
Summary: This article describes the fabrication of a low-cost PCR instrument with alternative processes, utilizing PCB heating elements, a computer fan for cooling, and PID control algorithm for temperature control. The device successfully amplified samples of T. pallidum and Zika virus RNA, showing similar performance to commercial PCR instruments.
BIOMEDICAL MICRODEVICES
(2021)
Article
Chemistry, Multidisciplinary
Ali Amadeh, Erfan Ghazimirsaeed, Amir Shamloo, Mahdi Dizani
Summary: Adding TiO2 nanoparticles in a photonic PCR system benefits gene amplification by improving heating and cooling rates while reducing energy consumption. The system demonstrates high temperature stability and accuracy, capable of amplifying eight gene samples simultaneously. The addition of TiO2 nanoparticles enhances thermal conductivity, leading to more efficient gene amplification.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Analytical
Linlin Zhuang, Jianbo Yang, Chunlei Song, Li Sun, Bin Zhao, Qiuping Shen, Xiyan Ren, Hongjing Shi, Yu Zhang, Mengling Zhu
Summary: An accurate and practical graphene oxide-based method, called GO-ASEA, was developed for rapid and highly sensitive detection of African swine fever virus (ASFV). This novel assay showed excellent specificity and selectivity, with a low detection limit, and demonstrated good clinical applicability. The GO-ASEA method has the potential to serve as a robust tool in epidemic prevention and point-of-care diagnosis.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Cheng Fang, Yuming Li, Song Hu, Hao Wang, Xiaoxia Chen, Xiaoli Zhu
Summary: The developed DNA tree in this study enables amplified analysis of mRNA in living cells, achieving sensitive, accurate, and nondestructive analysis. The probes are transported into cells by exosomes, are LDNAs resistant to degradation, laying the foundation for reliable analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)