Journal
ANALYTICAL BIOCHEMISTRY
Volume 393, Issue 2, Pages 215-221Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.06.029
Keywords
Cysteine; Hypoxia; Mass spectrometry; Oxygen sensing; Prolyl hydroxylase domain 2; Protein modification
Funding
- Newton-Abraham Fund
- Ad Futura
- Commonwealth Scholarship Scheme
- Engineering and Physical Sciences Research Council
- European Union
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council
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Prolyl hydroxylase domain 2 (PHD2) plays an important role in hypoxic sensing in humans. Here we report studies on the reactivity of cysteinyl residues of the catalytic domain of PHD2 using an approach in which nondenaturing electrospray ionization-mass spectrometry (ESI-MS) analyses were combined with the use of a thiol library and residue substitution. Among the seven cysteinyl residues of the PHD2 catalytic domain, Cys201 was found to be predominantly modified by thiols or N-ethylmaleimide. Selective modification of Cys201 was further demonstrated with methanethiosulfonate, a spin-labeled probe. The modified PHD2 will be useful in electron paramagnetic resonance studies on PHD2. The results demonstrate the use of a combined library/residue substitution/ ESI-MS approach for analyzing residue reactivity. (C) 2009 Elsevier Inc. All rights reserved.
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