Journal
ANALYTICAL BIOCHEMISTRY
Volume 395, Issue 2, Pages 244-248Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.08.002
Keywords
beta-Catenin; Co-immunoprecipitation; Protein-protein interaction; Miniaturization
Funding
- German Federal Ministry of Education (BMBF) [FKZ 313081E]
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Co-immunoprecipitation (co-IP) is a prominent technique for evaluating protein-protein interactions. Currently, large quantities of protein are required to perform co-IP followed by mass spectrometric or Western blot analyses of the interacting proteins. Here catenin-cadherin complexes were employed to establish a multiplexed microsphere-based co-immunoprecipitation (mu co-IP) protocol that allows the analysis of different complexes of a given protein with various interacting proteins within a single experiment using a limited amount of sample material. (C) 2009 Elsevier Inc. All rights reserved.
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