Article
Biochemistry & Molecular Biology
Emma K. Seipp, Rong Huang
Summary: A Proteolysis Targeting Chimera (PROTAC) is a bivalent molecule that promotes the degradation of specific proteins. In this study, a fluorescent probe ES148 with a binding affinity of 1.6 +/- 0.1 μM for FEM1C was designed and synthesized. A robust fluorescence polarization (FP) based competition assay was established to characterize FEM1C ligands, and the binding affinities were validated using isothermal titration calorimetry. This study offers new tools for PROTAC development by accelerating the discovery of FEM1C ligands.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Damian L. Stachura, Stephanie Nguyen, Steven W. Polyak, Blagojce Jovcevski, John B. Bruning, Andrew D. Abell
Summary: In this study, the synthesis and assay of N1-diphenylmethyl triazole-based analogues were reported, which showed potent inhibitory activity against Staphylococcus aureus. The findings have important clinical implications for combating blood-based infections caused by Staphylococcus aureus.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Analytical
Xiaoqi Wang, Shiwu Liu, Rong Xiao, Jue Hu, Ling Li, Yi Ning, Fangguo Lu
Summary: A graphene-oxide-based fluorescent bioassay was developed for determining agrC gene transcription in methicillin-resistant Staphylococcus aureus. The method utilizes target recycling amplification and hybridization chain reaction, resulting in enhanced fluorescence and sensitivity. This method can be applied for monitoring biofilm formation and studying drug action mechanisms.
Article
Chemistry, Medicinal
Damian L. Stachura, Stephanie Nguyen, Steven W. Polyak, Blagojce Jovcevski, John B. Bruning, Andrew D. Abell
Summary: The rise of multidrug-resistant bacteria has created an urgent need for new antibiotics, and one potential target is the enzyme biotin protein ligase (BPL). In this study, a new series of inhibitors of Staphylococcus aureus BPL (SaBPL) were designed, synthesized, and tested. Two particular compounds, triazoles 13 and 14, showed strong inhibition against SaBPL with Ki values of 10 +/- 2 and 30 +/- 6 nM, respectively, likely through hydrogen bonding interactions with amino acid residues S128 and N212 of SaBPL.
ACS MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Analytical
Qi-Hang Zhou, Xia Lv, Zhen-Hao Tian, Moshe Finel, Lei Feng, Peng-Chao Huo, Ya-Di Zhu, Yin Lu, Jie Hou, Guang-Bo Ge
Summary: Human UDP-glucuronosyltransferase enzymes play a crucial role in glucuronidation and detoxification. A novel fluorescence-based microplate assay was developed for screening inhibitors efficiently. Through extensive experiments, a suitable substrate was identified, the assay was successfully developed, and high-throughput screening of enzyme inhibitors was achieved.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Saimon M. M. Silva, Miaosi Li, Alexandre Xavier Mendes, Simon E. E. Moulton
Summary: The use of reagentless protein-based biosensors has the potential to revolutionize our understanding of biology and personalized health monitoring by directly monitoring molecular analytes in bodily fluids. Currently available molecular sensors are limited in their applicability to relevant biomarkers due to their reliance on specific enzymatic or chemical reactivity. A new class of reagentless protein-based electrochemical sensors has been introduced to overcome this limitation and enable direct measurements of biomarkers in unprocessed biological fluids. This mini-review discusses recent advances in the development of electroanalytical modular biosensors that integrate all the necessary components for autonomous functionality.
Article
Pharmacology & Pharmacy
Yihui Song, Min Zhao, Yahong Wu, Bin Yu, Hong-Min Liu
Summary: A high-throughput screening protocol was developed to discover SHP2 inhibitors, resulting in the identification of new SHP2-PTP inhibitors and allosteric inhibitors. These inhibitors showed potential for targeting cancer-associated SHP2 mutations.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Chemistry, Medicinal
George M. Ongwae, Irene Lepori, Mahendra D. Chordia, Brianna E. Dalesandro, Alexis J. Apostolos, M. Sloan Siegrist, Marcos M. Pires
Summary: Some dangerous bacterial pathogens deploy secondary membrane barriers to reduce influx of exogenous molecules. A new method, called bacterial chloro-alkane penetration assay (BaCAPA), using HaloTag protein, was developed to measure molecule uptake and accumulation in Gram-negative and mycobacterial species, as well as intracellular pathogens. BaCAPA enables measurement of permeability across four barriers.
ACS INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Xinjian Yin, Jiaxin Li, Senhua Chen, Yuping Wu, Zhigang She, Lan Liu, Yue Wang, Zhizeng Gao
Summary: A high-throughput FP-Tag OGT assay using BSA as a cost-effective tag was developed, identifying 2-methyleurotinone as a low-micromolar OGT inhibitor. This type of assay has potential applications with other glycosyltransferases.
Article
Biophysics
Mehdi Dadmehr, Monireh Mortezaei, Behnaz Korouzhdehi
Summary: In this study, a novel and sensitive method for the detection of MMP-9 enzyme was developed using a nanocomposite structure. The constructed nanocomposite exhibited a fluorescence enhancement and changes in surface plasmon resonance, enabling the semi-quantitative detection of MMP-9 enzyme. This approach provides an efficient, convenient, and practical tool for the simple identification and precise detection of MMP-9 enzyme.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Adithi C. Bhargava, Kathryn Mains, Albert Siu, Jie Gu, Jonathan Zarzar, Li Yi, Inn H. Yuk
Summary: A general esterase activity assay utilizing 4-methylumbelliferyl caprylate as the substrate was developed to monitor and characterize esterase activity during bioprocess development of monoclonal antibodies. The assay allows for rapid characterization of protein samples in under three hours, with the esterase activity directly correlating with polysorbate degradation, providing valuable information on degradation risk throughout drug development.
PHARMACEUTICAL RESEARCH
(2021)
Article
Infectious Diseases
Shuai-Bing Dong, Di Xiao, Jing-Yao Liu, Hui-Mei Bi, Zun-Rong Zheng, Li-Da Wang, Xiao-Wen Yang, Guo-Zhong Tian, Hong-Yan Zhao, Dong-Ri Piao, Zhi-Feng Xing, Hai Jiang
Summary: This study evaluated the value of Fluorescence Polarization Immunoassay (FPA) for the diagnosis of human brucellosis in China by recruiting 320 suspected cases. FPA showed high diagnostic value with a cutoff value of 88.5 mP, and when combined with ELISA, the sensitivity of diagnosis was significantly improved. FPA may have potential as a diagnostic method for human brucellosis in China.
INFECTIOUS DISEASES OF POVERTY
(2021)
Article
Biochemical Research Methods
Hang Zhang, Mengjing Xu, Haofeng Li, Xiaohan Mai, Jiawei Sun, Lan Mi, Jiong Ma, Xiangdong Zhu, Yiyan Fei
Summary: This study presents a series of optimization methods that greatly reduce the scanning time of OI-RD microscope images, resulting in a 10 times increase in detection speed. This makes the OI-RD microscope suitable for ultra-high throughput screening applications.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Multidisciplinary Sciences
Clemens Schulte, Vladimir Khayenko, Noah Frieder Nordblom, Franziska Tippel, Violetta Peck, Amit Jean Gupta, Hans Michael Maric
Summary: Protein-protein interactions are crucial for understanding physiology and pathology, with short linear motifs playing a key role. Current approaches for determining protein-peptide affinity have limitations, but the TRIC method presents a high-throughput way to analyze these interactions in solution. TRIC allows for the identification and mapping of protein interaction sites with varying affinities, providing a label-free method for determining binding affinities of unmodified peptide libraries.
Article
Biochemistry & Molecular Biology
Vlad Constantin Ursachi, Gianina Dodi, Alina Gabriela Rusu, Cosmin Teodor Mihai, Liliana Verestiuc, Vera Balan
Summary: The study focuses on the development of magnetic nanoparticles for cancer treatment, analyzing the structure, properties, and functionality, while also confirming their potential as drug delivery systems through detailed testing and evaluation.
Article
Chemistry, Medicinal
Xiuli Xu, Jiahui Han, Rui Lin, Steven W. Polyak, Fuhang Song
Article
Biochemistry & Molecular Biology
Kwang Jun Lee, William Tieu, Beatriz Blanco-Rodriguez, Ashleigh S. Paparella, Jingxian Yu, Andrew Hayes, Jiage Feng, Andrew C. Marshall, Benjamin Noll, Robert Milne, Danielle Cini, Matthew C. J. Wilce, Grant W. Booker, John B. Bruning, Steven W. Polyak, Andrew D. Abell
ACS CHEMICAL BIOLOGY
(2019)
Article
Chemistry, Medicinal
Yinhu Wang, Rawaf Alenzy, Di Song, Xingbang Liu, Yuetai Teng, Rumana Mowla, Yingang Ma, Steven W. Polyak, Henrietta Venter, Shutao Ma
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Oncology
Witchuda Sukjoi, Siraprapa Siritutsoontorn, Pakkanan Chansongkrow, Suppakit Waiwitlikhit, Steven W. Polyak, Malee Warnnissorn, Varodom Charoensawan, Chanitra Thuwajit, Sarawut Jitrapakdee
ANTICANCER RESEARCH
(2020)
Article
Chemistry, Medicinal
Yuan Qi Yeoh, John R. Horsley, Steven W. Polyak, Andrew D. Abell
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2020)
Article
Chemistry, Medicinal
Yuan Qi Yeoh, John R. Horsley, Jingxian Yu, Steven W. Polyak, Blagojce Jovcevski, Andrew D. Abell
Article
Biochemistry & Molecular Biology
Louise M. Sternicki, Stephanie Nguyen, Kamila J. Pacholarz, Perdita Barran, Nicole R. Pendini, Grant W. Booker, Yoann Huet, Rachel Baltz, Kate L. Wegener, Tara L. Pukala, Steven W. Polyak
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)
Article
Biochemistry & Molecular Biology
Xinjie Gu, Lily A. Pisoni, Yinhu Wang, Di Song, Matthew J. Sykes, Yinhui Qin, Susan J. Semple, Steven W. Polyak, Henrietta Venter, Shutao Ma
BIOORGANIC CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Emily J. Meyer, David J. Torpy, Anastasia Chernykh, Morten Thaysen-Andersen, Marni A. Nenke, John G. Lewis, Harinda Rajapaksha, Wayne Rankin, Steven W. Polyak
Article
Infectious Diseases
Wern Chern Chai, Jonathan J. Whittall, Di Song, Steven W. Polyak, Abiodun D. Ogunniyi, Yinhu Wang, Fangchao Bi, Shutao Ma, Susan J. Semple, Henrietta Venter
Article
Chemistry, Medicinal
Yinhu Wang, Rawaf Alenazy, Xinjie Gu, Steven W. Polyak, Panpan Zhang, Matthew J. Sykes, Na Zhang, Henrietta Venter, Shutao Ma
Summary: This study designed novel chromanone and 2H-benzo[h]chromene derivatives as potential efflux pump inhibitors targeting Escherichia coli AcrB. Results showed that compounds WK2, WL7, and WL10 exhibited broad-spectrum and high-efficiency efflux inhibitory activity, suggesting their potential as ideal AcrB inhibitors. Molecular modeling revealed that the strong pi-pi stacking interactions and hydrogen bond networks were key factors contributing to tight binding of AcrB.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Fisheries
Na Yang, Fuhang Song, Steven W. Polyak, Jianguo Liu
Summary: The study identified that the resistance mechanism of the V. anguillarum mutant to actinonin is associated with bacterial respiration.
Article
Chemistry, Medicinal
Xiuli Xu, Jiangpeng Li, Kai Zhang, Shangzhu Wei, Rui Lin, Steven W. Polyak, Na Yang, Fuhang Song
Summary: Nine new secondary metabolites, including six isocoumarin analogues and three compounds with a rare skeleton of isocoumarin coupled with phenylethylamine, were isolated and identified from the marine-derived fungus Paraphoma sp. CUGBMF180003. The chemical structures were determined using spectroscopic data, and two compounds showed strong antibacterial activity against Staphylococcus aureus.
Article
Communication
Lisa Bailey, Heather Bray
Summary: The recent conference of the Australian Science Communicators (ASC) association (15-17 February 2023) provided an opportunity for 140 delegates to reflect on the past decade of Inspiring Australia, the national strategy for public engagement with the sciences, and discuss the future role of science communicators in the Australian science and research landscape. The conference, which took place in Canberra, marked the first in-person gathering since the COVID-19 pandemic. Other topics of discussion included the role of AI in science communication and the significance of networks.
JCOM-JOURNAL OF SCIENCE COMMUNICATION
(2023)
Article
Communication
Linden Ashcroft, Mia Cobb, Lisa Bailey, Jenny Martin, Scott Daniel
JCOM-JOURNAL OF SCIENCE COMMUNICATION
(2020)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)