Article
Biochemical Research Methods
Jui-Chung Hong, Hao-Chun Fan, Po-Jiun Yang, Da-Wei Lin, Hsuan-Chen Wu, Hsiao-Chun Huang
Summary: The study shows that localized proteolysis can be employed to construct intracellular asymmetry in Escherichia coli, providing a new strategy for polarization in developmental cell biology. Circuits designed in this study may help to expand the synthetic biology repository for the engineering of synthetic morphogenesis.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biology
Shengchen Wang, Faying Zhang, Meng Mei, Ting Wang, Yueli Yun, Shihui Yang, Guimin Zhang, Li Yi
Summary: The study developed a fluorescence-assisted single-cell methodology (SPEC) to explore protein-protein interactions in E. coli cells, which offers high sensitivity, easy manipulation, and broad temperature adaptability.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemical Research Methods
Xianan Zhang, Nico Betterle, Diego Hidalgo Martinez, Anastasios Melis
Summary: This study investigates the stability of recombinant proteins in cyanobacteria and finds that heterologous proteins are more stable when in fusion constructs compared to when they are present alone. The results set a foundation for overexpression of pathway enzymes in synthetic biology approaches.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Fernando Merwaiss, Enrique Lozano-Sanchez, Joao Zulaica, Luciana Rusu, Marta Vazquez-Vilar, Diego Orzaez, Guillermo Rodrigo, Ron Geller, Jose-Antonio Daros
Summary: Researchers have developed genetically encoded multivalent viral nanoparticles that can recognize and neutralize SARS-CoV-2 Spike protein. These nanoparticles exhibited higher neutralization activity compared to the corresponding nanobodies in a dimeric format. The ability to produce VNP cocktails in plants makes them an attractive alternative to standard antibodies for multiple applications in diagnosis and therapeutics.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Maricarmen Marti, Fernando Merwaiss, Anamarija Butkovic, Jose-Antonio Daros
Summary: This study reports the production of genetically encoded plant-derived viral nanoparticles (VNPs) decorated with nanobodies. This system may provide an attractive alternative for sustainable production of nanobody-containing nanomaterials for diagnostic and therapeutic purposes in plants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Virology
John F. Murphy, H. Tucker Hallmark, Thiruvarangan Ramaraj, Anitha Sundararajan, Faye Schilkey, Aaron M. Rashotte
Summary: Through transcriptomic RNA sequencing, distinct profiles of Differentially Expressed Genes (DEGs) were identified for each TEV strain in infected pepper plants. The number of DEGs increased with the severity of disease symptoms, and the DEG patterns from 7 to 14 days post-inoculation corresponded to the severity of disease. Surprisingly, there were very few overlapping DEGs among the TEV strains, with no overlapping DEGs between all three strains at 7 or 14 days post-inoculation.
Article
Immunology
Ming-Hao Yang, Chung-Chi Hu, Chi-Hzeng Wong, Jian-Jong Liang, Hui-Ying Ko, Meng-Hsun He, Yi-Ling Lin, Na-Sheng Lin, Yau-Heiu Hsu
Summary: A new binary epitope-presenting CVP platform has been developed based on bamboo mosaic virus using SrtA-mediated ligation technology, showing promise for vaccine candidate development and mass production.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Doaa A. Goda, Ahmad R. Bassiouny, Nihad M. Abdel Monem, Nadia A. Soliman, Yasser R. Abdel-Fattah
Summary: The optimized parameters for incubating protein lysate from waste feathers using keratinase produced by Laceyella sacchari strain YNDH led to a significant increase in total protein content. The partially purified enzyme from this strain showed activity at high temperatures and pH levels, with characteristics suggesting a mixed serine-metallo enzyme type. The research also revealed the presence of essential and rare amino acids in the produced Feather Protein Lysate (FPL).
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yiwen Xu, Xiaoran Xuan, Renjun Gao, Guiqiu Xie
Summary: In this study, the extracellular expression of thermostable protease TTHA0724 was enhanced by developing a signal peptide library in the expression system of B. subtilis and optimizing signal peptides. The expression level of TTHA0724 in B. subtilis was 16.7 times higher compared to E. coli. TTHA0724 has great potential for industrial applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Aishi Dasgupta, Shon Gangai, Rishikesh Narayan, Shobhna Kapoor
Summary: The COVID-19 pandemic has generated research interest in identifying robust biomarkers and therapeutic targets. Lipids play a crucial role in SARS-CoV-2 pathogenesis, and targeting lipid-based processes may provide effective strategies for combating COVID-19.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Sea On Lee, Qi Xie, Stephen D. Fried
Summary: By rearranging the topology of a group I self-splicing intron from T4 bacteriophage, researchers successfully circularized mRNA to optimize protein expression. Through a modified PCR method generating mutants with higher expression levels, they demonstrated the potential of loopable translation technology for creating large peptide chains.
ACS CENTRAL SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Shuwei Peng, Chunlin Fang, Heng He, Xu Song, Xinghong Zhao, Yuanfeng Zou, Lixia Li, Renyong Jia, Zhongqiong Yin
Summary: This study found that myricetin can inhibit the replication of IBV by inhibiting the deubiquitinating activity of PLpro, therefore enhancing the host's immune response against IBV.
Article
Biochemistry & Molecular Biology
Joanna Pianka, Natalia Gruba, Adam Lesner
Summary: West Nile Virus (WNV), a member of the flavivirus group, can cause mild or severe forms of infection, with no known medications for prevention. The research aimed to develop specific tools to determine the activity of the WNV serine proteinase.
BIOORGANIC CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Shiyu Chen, Scott Lovell, Sumin Lee, Matthias Fellner, Peter D. Mace, Matthew Bogyo
Summary: A phage display method was developed to screen for covalent inhibitors that bind to protein targets by introducing a reactive linker to form cyclic peptides and an electrophilic 'warhead' to react with target nucleophiles. This approach identified cyclic peptides that irreversibly inhibited cysteine protease and serine hydrolase with high specificity and potency. It enables rapid, unbiased screening to identify new classes of highly selective covalent inhibitors for diverse molecular targets.
NATURE BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Muhammad Usman Mirza, Ida Alanko, Michiel Vanmeert, Kendall M. Muzzarelli, Outi M. H. Salo-Ahen, Iskandar Abdullah, Iulia A. Kovari, Sandra Claes, Steven De Jonghe, Dominique Schols, Raymond F. Schinazi, Ladislau C. Kovari, John F. Trant, Sarfraz Ahmad, Matheus Froeyen
Summary: This study used a computer-aided structure-based approach to screen a library of compounds against ZIKV NS2B-NS3 protease. Several compounds showed promising activity against the protease, and one compound also exhibited anti-ZIKV activity in whole cells. This research provides a promising starting point for the development of novel compounds against ZIKV.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)